Anti-WSX-1 抗体 (ab5996)
Key features and details
- Rabbit polyclonal to WSX-1
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-WSX-1 antibody
WSX-1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to WSX-1 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-P, WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human WSX-1 aa 611-625 (C terminal).
Sequence:SGYEKHFLPTPEELG
(Peptide available asab39872) -
ポジティブ･コントロール
- Human spleen tissue lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab5996の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use a concentration of 20 µg/ml.
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| IHC-P |
Use a concentration of 5 µg/ml.
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| WB |
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 70 kDa.
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| 特記事項 |
|---|
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ICC/IF
Use a concentration of 20 µg/ml. |
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IHC-P
Use a concentration of 5 µg/ml. |
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WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 70 kDa. |
ターゲット情報
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機能
Receptor for IL27. Requires IL6ST/gp130 to mediate signal transduction in response to IL27. This signaling system acts through STAT3 and STAT1. Involved in the regulation of Th1-type immune responses. Also appears to be involved in innate defense mechanisms. -
組織特異性
Highly expressed in lymphoid tissues such as spleen, lymph nodes and peripheral blood leukocytes. Weakly expressed in other tissues examined including heart, brain, fetal and adult lung, liver, skeletal muscle, kidney, pancreas, prostate, testis, ovary, small intestine, kidney and colon. In the lymphoid system, higher level expression in CD4+ T-cell subsets than in CD8+ T-cell subsets. Also weaker expression in CD19+ B-cells and monocytes. -
配列類似性
Belongs to the type I cytokine receptor family. Type 2 subfamily.
Contains 3 fibronectin type-III domains. -
ドメイン
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 9466 Human
- Omim: 605350 Human
- SwissProt: Q6UWB1 Human
- Unigene: 132781 Human
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別名
- Class I cytokine receptor antibody
- CRL1 antibody
- Cytokine receptor-like 1 antibody
see all
画像
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Western blot - Anti-WSX-1 antibody (ab5996)Anti-WSX-1 antibody (ab5996) at 1 µg/ml + Human spleen tissue lysate
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WSX-1 antibody (ab5996)ab5996 at 5µg/ml staining WSX-1 in human spleen tissue by IHC
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Immunocytochemistry/ Immunofluorescence - Anti-WSX-1 antibody (ab5996)Immunofluorescence of WSX-1 in Human Spleen cells using ab5996 at 20 ug/ml.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab5996 は 2 報の論文で使用されています。
- Sugiyama N et al. Amelioration of human lupus-like phenotypes in MRL/lpr mice by overexpression of interleukin 27 receptor alpha (WSX-1). Ann Rheum Dis 67:1461-7 (2008). WB ; Mouse . PubMed: 18094002
- Takeda A et al. WSX-1 over-expression in CD4(+) T cells leads to hyperproliferation and cytokine hyperproduction in response to TCR stimulation. Int Immunol 17:889-97 (2005). PubMed: 15908443