Anti-WRNIP1/WHIP 抗体 (ab4731)
Key features and details
- Rabbit polyclonal to WRNIP1/WHIP
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-WRNIP1/WHIP antibody
WRNIP1/WHIP 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to WRNIP1/WHIP -
由来種
Rabbit -
特異性
Reactivity is expected against human, mouse, rat and monkey WHIP1 protein.
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アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 0.424% Tripotassium orthophosphate, 0.88% Sodium chloride -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide immobilized to a solid phase. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab4731の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
| 特記事項 |
|---|
ICC/IF: Use at an assay dependent dilution (PMID 17888034).
WB: 1/200. Detects a band of approximately 97 kDa (predicted molecular weight: 72 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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機能
Functions as a modulator for initiation or reinitiation events during DNA polymerase delta-mediated DNA synthesis. Has an intrinsic ATPase activity that functions as a sensor of DNA damage or of arrested replication forks and regulates the extent of DNA synthesis. -
組織特異性
Ubiquitously expressed. -
配列類似性
Belongs to the AAA ATPase family.
Contains 1 UBZ-type zinc finger. -
翻訳後修飾
Sumoylated by SUMO1 and SUMO2/3. -
細胞内局在
Nucleus. Colocalizes with WRN in granular structures in the nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 56897 Human
- Entrez Gene: 78903 Mouse
- Entrez Gene: 282835 Rat
- Omim: 608196 Human
- SwissProt: Q96S55 Human
- SwissProt: Q91XU0 Mouse
- SwissProt: Q8CG07 Rat
- Unigene: 236828 Human
see all -
別名
- ATPase WRNIP 1 antibody
- ATPase WRNIP1 antibody
- bA420G6.2 antibody
see all
画像
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Western blot - Anti-WRNIP1/WHIP antibody (ab4731)
Western blot using ab4731 on HEK 293 lysate at 1/200.
~30µg of lysate was loaded per lane for 4-20% gradient SDS-PAGE. Comparison to a molecular weight marker (not shown) indicates a primary band of ~96.0 kDa is detected (indicated by an arrow). The identity of the minor band migrating at a slightly higher molecular weight is unknown, but may represent an alternate isoform of WRNIP1/WHIP or post translational modification of the WRNIP1/WHIP protein. The main band could be blocked using the immunising peptide.
Western blot using ab4731 on HEK 293 lysate at 1/200.
~30µg of lysate was loaded per lane for 4-20% gradient SDS-PAGE. Comparison to a molecular weight marker (not shown) indicates a primary band of ~96.0 kDa is detected (indicated by an arrow). The identity of the minor band migrating at a slightly higher molecular weight is unknown, but may represent an alternate isoform of WRNIP1/WHIP or post translational modification of the WRNIP1/WHIP .
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab4731 は 3 報の論文で使用されています。
- Ciuffa R et al. Novel biochemical, structural, and systems insights into inflammatory signaling revealed by contextual interaction proteomics. Proc Natl Acad Sci U S A 119:e2117175119 (2022). PubMed: 36179048
- Kanu N et al. RAD18, WRNIP1 and ATMIN promote ATM signalling in response to replication stress. Oncogene 35:4009-19 (2016). PubMed: 26549024
- Mano Y et al. Fibroblast growth factor receptor 1 oncogene partner as a novel prognostic biomarker and therapeutic target for lung cancer. Cancer Sci 98:1902-13 (2007). ICC/IF ; Human . PubMed: 17888034