Anti-Wnt6 抗体 [EPR9244] (ab154144)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9244] to Wnt6
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Wnt6 antibody [EPR9244]
Wnt6 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR9244] to Wnt6 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt,ICC/IF,IHC-P or IP -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human Wnt6.
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ポジティブ・コントロール
- HeLa, Jurkat, SH-SY5Y, C6, RAW 264.7, PC12, NIH 3T3 and Neuro 2a cell lysates.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20ºC. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR9244 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154144の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
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特記事項 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 40 kDa. |
ターゲット情報
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機能
Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. -
配列類似性
Belongs to the Wnt family. -
翻訳後修飾
Palmitoylation at Ser-228 is required for efficient binding to frizzled receptors. It is also required for subsequent palmitoylation at Cys-76. Palmitoylation is necessary for proper trafficking to cell surface. -
細胞内局在
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 7475 Human
- Entrez Gene: 22420 Mouse
- Entrez Gene: 316526 Rat
- Omim: 604663 Human
- SwissProt: Q9Y6F9 Human
- SwissProt: P22727 Mouse
- Unigene: 29764 Human
- Unigene: 268282 Mouse
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別名
- Protein Wnt 6 antibody
- Protein Wnt-6 antibody
- Protein Wnt-6 precursor antibody
see all
画像
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All lanes : Anti-Wnt6 antibody [EPR9244] (ab154144) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : SH-SY5Y cell lysate
Lane 4 : C6 cell lysate
Lane 5 : RAW 264.7 cell lysate
Lane 6 : PC12 cell lysate
Lane 7 : NIH 3T3 cell lysate
Lane 8 : Neuro 2a cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 40 kDa
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab154144 は 3 報の論文で使用されています。
- Gao X et al. Roles of WNT6 in Sheep Endometrial Epithelial Cell Cycle Progression and Uterine Glands Organogenesis. Vet Sci 8:N/A (2021). PubMed: 34941843
- Gonçalves CS et al. WNT6 is a novel oncogenic prognostic biomarker in human glioblastoma. Theranostics 8:4805-4823 (2018). PubMed: 30279739
- Jiang Z et al. Wnt6 influences the viability of mouse embryonic palatal mesenchymal cells via the ß-catenin pathway. Exp Ther Med 14:5339-5344 (2017). PubMed: 29285061