Anti-Wnt3a 抗体 [3A6] (ab81614)
![Western blot - Anti-Wnt3a antibody [3A6] (ab81614)](https://www.abcam.co.jp/ps/products/81/ab81614/Images/ab81614-338983-anti-wnt3a-antibody-3a6-western-blot.jpg "Western blot - Anti-Wnt3a antibody [3A6] (ab81614)")
Key features and details
- Mouse monoclonal [3A6] to Wnt3a
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG2a
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Wnt3a antibody [3A6]
Wnt3a 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [3A6] to Wnt3a -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length human Wnt3a (aa19-352) purified from E. coli (NP_149122).
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ポジティブ･コントロール
- WB: A549 cell lysates, Wnt3a transfected 293T cell lysate .
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特記事項
This product was changed from ascites to tissue culture supernatant on 24/01/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Ammonium Sulphate Precipitation -
ポリ/モノ
モノクローナル -
クローン名
3A6 -
アイソタイプ
IgG2a -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab81614の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
Use at an assay dependent concentration. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
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| 特記事項 |
|---|
|
WB
Use at an assay dependent concentration. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa). |
ターゲット情報
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機能
Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube. -
組織特異性
Moderately expressed in placenta and at low levels in adult lung, spleen, and prostate. -
配列類似性
Belongs to the Wnt family. -
細胞内局在
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 89780 Human
- Omim: 606359 Human
- SwissProt: P56704 Human
- Unigene: 336930 Human
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別名
- Protein Wnt 3a Precursor antibody
- Protein Wnt-3a antibody
- Wingless type MMTV integration site family member 3A antibody
see all
画像
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Western blot - Anti-Wnt3a antibody [3A6] (ab81614)All lanes : Anti-Wnt3a antibody [3A6] (ab81614) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : Wnt3a transfected 293T cell lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : goat anti-mouse secondary antibody conjugated to HRP
Predicted band size: 39 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (17)
ab81614 は 17 報の論文で使用されています。
- Wu CL et al. Single cell transcriptomic analysis of human pluripotent stem cell chondrogenesis. Nat Commun 12:362 (2021). PubMed: 33441552
- Zhang H et al. TGFB3-AS1 promotes Hcy-induced inflammationof macrophages via inhibiting the maturityof miR-144 and upregulating Rap1a. Mol Ther Nucleic Acids 26:1318-1335 (2021). PubMed: 34853730
- Wen B et al. Overexpression of microRNA-221 promotes the differentiation of stem cells from human exfoliated deciduous teeth to neurons through activation of Wnt/β-catenin pathway via inhibition of CHD8. Cell Cycle 19:3231-3248 (2020). PubMed: 33198579
- Tian X et al. Long noncoding RNA LINC00662 promotes M2 macrophage polarization and hepatocellular carcinoma progression via activating Wnt/ß-catenin signaling. Mol Oncol 14:462-483 (2020). PubMed: 31785055
- Ackers I et al. Oxidized Low-Density Lipoprotein Induces WNT5A Signaling Activation in THP-1 Derived Macrophages and a Human Aortic Vascular Smooth Muscle Cell Line. Front Cardiovasc Med 7:567837 (2020). PubMed: 33330641