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Anti-Wnt3 抗体 (ab50341)

Submit a review Q&A (1)References (3)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt3 antibody (ab50341)
  • Western blot - Anti-Wnt3 antibody (ab50341)

Key features and details

  • Goat polyclonal to Wnt3
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-Wnt3 antibody
    Wnt3 一次抗体 製品一覧

  • 製品の詳細

    Goat polyclonal to Wnt3

  • 由来種

    Goat

  • アプリケーション

    適用あり: WB, IHC-Pmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Rat, Chicken, Dog

  • 免疫原

    Synthetic peptide:

    CGRGHNTRTEKRKEK

    , corresponding to internal sequence amino acids 315-329 of Human Wnt3
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • バッファー

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% Tris buffered saline, 0.5% BSA
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • 特記事項（精製）

    This antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab50341の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 40 kDa).

1 hour primary incubation is recommended for this product.
IHC-P
Use a concentration of 2 - 4 µg/ml.
特記事項
WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 40 kDa).

1 hour primary incubation is recommended for this product.
IHC-P
Use a concentration of 2 - 4 µg/ml.

ターゲット情報

  • 機能

    Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube.

  • 関連疾患

    Defects in WNT3 are the cause of autosomal recessive tetra-amelia (ARTTRA) [MIM:273395]. Tetra-amelia is a rare human genetic disorder characterized by complete absence of all four limbs and other anomalies such as craniofacial, nervous system, pulmonary, skeletal and urogenital defects.

  • 配列類似性

    Belongs to the Wnt family.

  • 細胞内局在

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Int 4 antibody
      • INT4 antibody
      • MGC131950 antibody
      • MGC138321 antibody
      • MGC138323 antibody
      • Proto-oncogene Int-4 homolog antibody
      • Proto-oncogene Wnt-3 antibody
      • wingless type MMTV integration site family member 3 antibody
      • Wnt 3 proto oncogene protein antibody
      • WNT 3 proto oncogene protein precursor antibody
      • wnt3 antibody
      • WNT3_HUMAN antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt3 antibody (ab50341)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt3 antibody (ab50341)

      ab50341 (2µg/ml) staining of paraffin embedded Human Breast shows cytoplasm staining in some luminal epithelial cells following steamed antigen retrieval with citrate buffer pH 6 and HRP-staining.

    • Western blot - Anti-Wnt3 antibody (ab50341)
      Western blot - Anti-Wnt3 antibody (ab50341)
      Anti-Wnt3 antibody (ab50341) at 1 µg/ml + Human Brain lysate (35µg protein in RIPA buffer).

      Predicted band size: 40 kDa
      Observed band size: 38 kDa why is the actual band size different from the predicted?
      Additional bands at: 17 kDa. We are unsure as to the identity of these extra bands.



      Primary incubation was 1 hour. Detected by chemiluminescence.

    参考文献 (3)

    ab50341 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab50341 は 3 報の論文で使用されています。

    • Luo W  et al. c-Myc inhibits myoblast differentiation and promotes myoblast proliferation and muscle fibre hypertrophy by regulating the expression of its target genes, miRNAs and lincRNAs. Cell Death Differ 26:426-442 (2019). PubMed: 29786076
    • Tripathy A  et al. The molecular connection of histopathological heterogeneity in hepatocellular carcinoma: A role of Wnt and Hedgehog signaling pathways. PLoS One 13:e0208194 (2018). PubMed: 30513115
    • Lee YS  et al. Microbiota-Derived Lactate Accelerates Intestinal Stem-Cell-Mediated Epithelial Development. Cell Host Microbe 24:833-846.e6 (2018). PubMed: 30543778

    レビューと Q&A

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    レビューを送る 質問を送る

    Question

    might use ab32249, ab50341, ab116222 in mouse and ChIP and IHC-whole mount send protocol for IHC whole mount

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    Answer

    Thank you for contacting us. Here are the links to our Whole mount staining protocols - Protocols and troubleshooting tips for whole mount immunohistochemical staining: https://www.abcam.com/index.html?pageconfig=resource&rid=11330 and for the mouse staining in particular (also attached): https://www.abcam.com/ps/pdf/protocols/whole_mount_mouse_chick.pdf Please let me know if you like to receive the testing discounts for the 3 antibodies. Here is some more information in that regard: For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works: The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody at the full price (100%) of the antibody you have tested. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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