AB109437

Anti-Wnt16 抗体 [EPR6064]

Anti-Wnt16 antibody [EPR6064]

RabMAb
Recombinant
詳細を見る

(2 Reviews)

(10 Publications)

Rabbit Recombinant Monoclonal WNT16 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 10 publications.

別名を表示する

Protein Wnt-16, WNT16

3 Images

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Western blot - Anti-Wnt16 antibody [EPR6064] (AB109437)

All lanes:

Western blot - Anti-Wnt16 antibody [EPR6064] (ab109437) at 1/1000 dilution

Lane 1:

LnCaP cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

Raji cell lysate at 10 µg

Lane 4:

293T cell lysate at 10 µg

Predicted band size: 41 kDa

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Lab

Western blot - Anti-Wnt16 antibody [EPR6064] (AB109437)

All lanes:

Western blot - Anti-Wnt16 antibody [EPR6064] (ab109437) at 1/1000 dilution

Lane 1:

Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate at 20 µg

Lane 2:

Mouse brain tissue lysate at 20 µg

Lane 3:

Rat brain tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 41 kDa

Observed band size: 41 kDa

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OI-RD Scanning - Anti-Wnt16 antibody [EPR6064] (AB109437)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-Wnt16 antibody [EPR6064] (AB109437)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR6064

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Rat, Human

アプリケーション

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

保管に関する情報

Stable for 12 months at -20°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Wnt16 also known as wingless-type MMTV integration site family member 16 is a protein with a mass of approximately 43 kDa. It belongs to the Wnt protein family which comprises secreted signaling proteins. Wnt16 facilitates the transfer of signals between cells influencing various cellular processes. This protein shows expression in several tissues such as bone cartilage and hematopoietic tissues playing roles in maintaining their functions.

Biological function summary

Wnt16 influences bone development and maintenance by enhancing osteoblast proliferation and differentiation. It functions as part of a complex signaling cascade interacting with receptors on cell surfaces to initiate intracellular signaling pathways. One of its primary roles is in balancing bone formation and resorption ensuring skeletal integrity. Furthermore Wnt16 contributes to cartilage homeostasis preventing degenerative changes that could lead to diseases like osteoarthritis.

Pathways

Wnt16 is part of significant signaling pathways like the canonical Wnt/β-catenin pathway as well as the non-canonical Wnt pathways. In the canonical pathway Wnt16 binds to Frizzled receptors and LRP5/6 co-receptors facilitating the stabilization and translocation of β-catenin into the nucleus affecting gene transcription. In this context Wnt16 relates closely to other Wnt proteins like Wnt3a influencing diverse cellular outcomes such as proliferation and differentiation.

Wnt16 has an impact on conditions like osteoporosis and osteoarthritis. In osteoporosis the reduction in Wnt16 expression correlates with decreased bone density and increased risk of fractures. Through its interaction with bone-forming proteins like Osteocalcin Wnt16 regulates bone mass and strength. Additionally in osteoarthritis Wnt16 downregulation leads to increased cartilage degradation linking it to proteins like MMP-13 which is involved in cartilage breakdown.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters (By similarity).

See full target information Protein Wnt-16

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文献 (10)

Recent publications for all applications. Explore the full list and refine your search

American journal of translational research 15:5047-5070 PubMed37692966

2023

Matrine improves the hepatic microenvironment and reverses epithelial-mesenchymal transition in hepatocellular carcinoma by inhibiting the Wnt-1/β-catenin signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jianfei Shi,Hua Zhang,Guangjie Han,Jinfeng Wang,Xin Han,Man Zhao,Xiaoling Duan,Lili Mi,Ning Li,Xiaolei Yin,Cuizhen Li,Fei Yin

Developmental neuroscience 44:131-141 PubMed34929706

2021

Circ-Ctnnb1 Regulates Neuronal Injury in Spinal Cord Injury through the Wnt/β-Catenin Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jialong Qi,Tao Wang,Zhidong Zhang,Zongsheng Yin,Yiming Liu,Li Ma,Shaobao Pei,Zhou Dong,Guosong Han

Human mutation 42:37-49 PubMed33058301

2020

USF3 modulates osteoporosis risk by targeting WNT16, RANKL, RUNX2, and two GWAS lead SNPs rs2908007 and rs4531631.

Applications

Unspecified application

Species

Unspecified reactive species

Weiyuan Ye,Ya Wang,Sasa Hou,Bing Mei,Xinhong Liu,Han Huang,Qian Zhou,Yajing Niu,Yuanyuan Chen,Manling Zhang,Qingyang Huang

Frontiers in immunology 10:2281 PubMed31608065

2019

Hsa_circ_0000479 as a Novel Diagnostic Biomarker of Systemic Lupus Erythematosus.

Applications

Unspecified application

Species

Unspecified reactive species

Gangqiang Guo,Huijing Wang,Lele Ye,Xinyu Shi,Kejing Yan,Kangmin Lin,Qunjia Huang,Baoqing Li,Qiaoai Lin,Lejiang Zhu,Xiangyang Xue,Huidi Zhang

Journal of extracellular vesicles 7:1490144 PubMed30034643

2018

Molecular subtypes and differentiation programmes of glioma stem cells as determinants of extracellular vesicle profiles and endothelial cell-stimulating activities.

Applications

Unspecified application

Species

Unspecified reactive species

C Spinelli,L Montermini,B Meehan,A R Brisson,S Tan,D Choi,I Nakano,J Rak

Scientific reports 8:8711 PubMed29880826

2018

Glucocorticoids suppress Wnt16 expression in osteoblasts in vitro and in vivo.

Applications

Species

Mouse

Susanne Hildebrandt,Ulrike Baschant,Sylvia Thiele,Jan Tuckermann,Lorenz C Hofbauer,Martina Rauner

American journal of respiratory cell and molecular biology 59:65-76 PubMed29345973

2018

R-Spondin-2 Is Upregulated in Idiopathic Pulmonary Fibrosis and Affects Fibroblast Behavior.

Applications

Unspecified application

Species

Unspecified reactive species

Adrián Munguía-Reyes,Yalbi I Balderas-Martínez,Carina Becerril,Marco Checa,Remedios Ramírez,Blanca Ortiz,Jorge Meléndez-Zajgla,Annie Pardo,Moisés Selman

Journal of Cancer 9:614-628 PubMed29483967

2018

The role of cancer-associated fibroblast MRC-5 in pancreatic cancer.

Applications

Species

Human

Song-Ming Ding,Ai-Li Lu,Wu Zhang,Lin Zhou,Hai-Yang Xie,Shu-Sen Zheng,Qi-Yong Li

BioMed research international 2015:731938 PubMed26339636

2015

Similarities in Gene Expression Profiles during In Vitro Aging of Primary Human Embryonic Lung and Foreskin Fibroblasts.

Applications

Species

Unspecified reactive species

Shiva Marthandan,Steffen Priebe,Mario Baumgart,Marco Groth,Alessandro Cellerino,Reinhard Guthke,Peter Hemmerich,Stephan Diekmann

PloS one 7:e48884 PubMed23155423

2012

Impaired skeletal muscle regeneration in the absence of fibrosis during hibernation in 13-lined ground squirrels.

Applications

Species

Owl

Eva Andres-Mateos,Rebeca Mejias,Arshia Soleimani,Brian M Lin,Tyesha N Burks,Ruth Marx,Benjamin Lin,Richard C Zellars,Yonggang Zhang,David L Huso,Tom G Marr,Leslie A Leinwand,Dana K Merriman,Ronald D Cohn

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Wnt16 抗体 [EPR6064]

Anti-Wnt16 antibody [EPR6064]

Western blot - Anti-Wnt16 antibody [EPR6064] (AB109437)

Western blot - Anti-Wnt16 antibody [EPR6064] (AB109437)

OI-RD Scanning - Anti-Wnt16 antibody [EPR6064] (AB109437)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (10)

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