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AB31962

Anti-Wnt11 抗体

Anti-Wnt11 antibody

4

(8 Reviews)

|

(19 Publications)

Rabbit Polyclonal WNT11 antibody. Suitable for WB, ICC/IF and reacts with Recombinant fragment - Human, Human samples. Cited in 19 publications.

別名を表示する

Protein Wnt-11, WNT11

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Wnt11 antibody (AB31962)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Wnt11 antibody (AB31962)

ab31962 staining Wnt11 in HaCat cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab31962 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue). Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

Western blot - Anti-Wnt11 antibody (AB31962)
  • WB

Supplier Data

Western blot - Anti-Wnt11 antibody (AB31962)

Blocking Buffer : 2% BSA

All lanes:

Western blot - Anti-Wnt11 antibody (ab31962) at 1 µg/mL

All lanes:

Human Wnt11 Recombinant Protein at 0.01 µg

Secondary

All lanes:

Goat polyclonal to rabbit IgG - H&L - Pre Adsorbed (HRP) at 1/50000 dilution

Predicted band size: 39 kDa

Observed band size: 39 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

ICC/IF, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特異性

From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.

This antibody detects recombinant human Wnt11 by Western Blotting. We have been unable to detect endogenous protein in cell lines with this antibody, possibly due to the low level of endogenous protein in the samples tested.

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Reactivity data

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下記製品にもご興味をお持ちいただけるかもしれません:

AB317552

Human Wnt11 ELISA Kit

0

0 Reviews

製品ページを見る
こちらの製品も推奨する理由

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
バッファー組成
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 98.98% PBS, 1% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Wnt11 also known as Wingless-type MMTV integration site family member 11 is a signaling protein weighing approximately 40 kDa. It is a member of the Wnt family which consists of secreted glycoproteins characterized by their cysteine-rich structure. Wnt11 regulates cellular processes and is expressed in various tissues including heart and developing limbs. This protein plays a role in cellular communication and influences cell fate determinations during early development.
Biological function summary

The signaling protein Wnt11 modulates diverse cellular processes including cell polarity and migration. Wnt11 is part of a group of proteins that orchestrate cellular signaling without forming a stable complex. It influences the planar cell polarity (PCP) pathway which affects how cells align themselves in the tissue plane. Wnt11's interaction with other Wnt proteins further diversifies its role in developmental signaling impacting processes such as gastrulation and organ development.

Pathways

Wnt11 influences key biological pathways vital for embryogenesis and tissue homeostasis. Wnt11 participates in the non-canonical Wnt signaling pathway like the PCP pathway facilitating cytoskeletal rearrangements and cell adhesion. Wnt11 often interacts with related proteins such as Dishevelled and Frizzled receptors. Additionally Wnt11 influences cardiac morphogenesis and is distinct from the canonical Wnt/beta-catenin pathway emphasizing its role in developmental processes independent of gene transcription activation.

Wnt11 impacts cardiac development and skeletal abnormalities. Defective Wnt11 signaling associates with congenital heart defects as its disruption alters heart tube formation. Associations also exist with craniofacial anomalies. Relevant proteins intersecting with these disorders include Vangl2 in PCP signaling indicating Wnt11's role in ensuring normal organ architecture. By influencing cellular migration and alignment Wnt11 contributes to understanding developmental disorders and potential therapeutic targets.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.
See full target information WNT11
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文献 (19)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 13:13331 PubMed37587207

2023

Hedyotis diffusa-Sculellaria barbata (HD-SB) suppresses the progression of colorectal cancer cells via the hsa_circ_0039933/hsa-miR-204-5p/wnt11 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Danye Zhu,Shanmin Yuan,Cong Chen

The European respiratory journal : PubMed32859680

2020

Wnt5a and Wnt11 as acute respiratory distress syndrome biomarkers for SARS-CoV-2 patients.

Applications

Unspecified application

Species

Unspecified reactive species

Eun Young Choi,Hee Ho Park,Hyelim Kim,Hong Nam Kim,Inyoung Kim,Soyoung Jeon,Wantae Kim,Jong-Sup Bae,Wonhwa Lee

The Journal of clinical investigation 130:4152-4166 PubMed32597829

2020

Salt causes aging-associated hypertension via vascular Wnt5a under Klotho deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Wakako Kawarazaki,Risuke Mizuno,Mitsuhiro Nishimoto,Nobuhiro Ayuzawa,Daigoro Hirohama,Kohei Ueda,Fumiko Kawakami-Mori,Shigeyoshi Oba,Takeshi Marumo,Toshiro Fujita

Molecular medicine reports 22:1839-1846 PubMed32582982

2020

Tanshinone IIA promotes cardiac differentiation and improves cell motility by modulating the Wnt/β‑catenin signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Kun Li,Xiuyan Wang,Chenxing Fan,Chunxia Wu,Shizheng Li,Hua Liu

Cell reports 30:771-782.e6 PubMed31968252

2020

Chromosome 3q26 Gain Is an Early Event Driving Coordinated Overexpression of the PRKCI, SOX2, and ECT2 Oncogenes in Lung Squamous Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Liu,Ning Yin,Xue Wang,Andras Khoor,Vaishnavi Sambandam,Anwesha B Ghosh,Zoe A Fields,Nicole R Murray,Verline Justilien,Alan P Fields

Experimental and therapeutic medicine 18:4631-4636 PubMed31798700

2019

Nkx2.5 insufficiency leads to atrial electrical remodeling through Wnt signaling in HL-1 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jingjing Chen,Shunen Xu,Wei Li,Lirong Wu,Long Wang,Yongkang Li,Wei Zhou

Cellular physiology and biochemistry : internation 49:2254-2263 PubMed30257243

2018

Hyperglycemia Altered the Fate of Cardiac Stem Cells to Adipogenesis through Inhibiting the β-Catenin/TCF-4 Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoming Zhang,Ke Meng,Yujie Pu,Chao Wang,Yingying Chen,Linlin Wang

Journal of extracellular vesicles 7:1490144 PubMed30034643

2018

Molecular subtypes and differentiation programmes of glioma stem cells as determinants of extracellular vesicle profiles and endothelial cell-stimulating activities.

Applications

Unspecified application

Species

Unspecified reactive species

C Spinelli,L Montermini,B Meehan,A R Brisson,S Tan,D Choi,I Nakano,J Rak

Biomedicine & pharmacotherapy = Biomedecine & phar 106:483-490 PubMed29990836

2018

MicroRNA-298 represses hepatocellular carcinoma progression by inhibiting CTNND1-mediated Wnt/β-catenin signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Ningjia Cao,Liang Mu,Wei Yang,Li Liu,Liang Liang,Hong Zhang

Scientific reports 6:35562 PubMed27765945

2016

Different populations of Wnt-containing vesicles are individually released from polarized epithelial cells.

Applications

WB

Species

Human

Qiuhong Chen,Ritsuko Takada,Chiyo Noda,Satoru Kobayashi,Shinji Takada
View all publications
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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。
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関連製品

Select an associated product type
Alternative Product
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1

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