Anti-WNK1 抗体 [EPR24632-107] - BSA and Azide free (Capture) (ab302753)
![Sandwich ELISA - Anti-WNK1 antibody [EPR24632-107] - BSA and Azide free (Capture) (ab302753)](https://www.abcam.co.jp/ps/products/302/ab302753/Images/ab302753-2-human-mouse-wnk1-antibody-pair-sandwich-elisa-standard-curve-2.jpg "Sandwich ELISA - Anti-WNK1 antibody [EPR24632-107] - BSA and Azide free (Capture) (ab302753)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24632-107] to WNK1 - BSA and Azide free (Capture)
- Suitable for: Sandwich ELISA
- Reacts with: Mouse, Human
製品の概要
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製品名
Anti-WNK1 antibody [EPR24632-107] - BSA and Azide free (Capture)
WNK1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR24632-107] to WNK1 - BSA and Azide free (Capture) -
由来種
Rabbit -
アプリケーション
適用あり: Sandwich ELISAmore details -
種交差性
交差種: Mouse, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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特記事項
ab302753 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human and Mouse WNK1. The recommended pair for sandwich ELISA is:
Capture: ab302753, Human, mouse WNK1 Capture Antibody (unconjugated)
Detector: ab302754, Human, mouse WNK1 Detector Antibody (unconjugated)
The reference range value is 0.08 - 5 pg/mL.Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Constituent: 100% PBS -
キャリア・フリー
はい -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR24632-107 -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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sELISA pair antibody
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab302753の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Streptavidin Conjugation Kit - Lightning-Link® (ab102921) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795) and Rabbit monoclonal [EPR24632-117] to WNK1 - BSA and Azide free (Detector) (ab302754).
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| 特記事項 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Streptavidin Conjugation Kit - Lightning-Link® (ab102921) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795) and Rabbit monoclonal [EPR24632-117] to WNK1 - BSA and Azide free (Detector) (ab302754). |
ターゲット情報
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機能
Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D and SGK1. Controls sodium and chloride ion transport by inhibiting the activity of WNK4, by either phosphorylating the kinase or via an interaction between WNK4 and the autoinhibitory domain of WNK1. WNK4 regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation. WNK1 may also play a role in actin cytoskeletal reorganization. Phosphorylates NEDD4L. Acts as a scaffold to inhibit SLC4A4, SLC26A6 as well as CFTR activities and surface expression, recruits STK39 which mediates the inhibition. -
組織特異性
Widely expressed, with highest levels observed in the testis, heart, kidney and skeletal muscle. Isoform 3 is kidney-specific and specifically expressed in the distal convoluted tubule (DCT) and connecting tubule (CNT) of the nephron. -
関連疾患
Pseudohypoaldosteronism 2C
Neuropathy, hereditary sensory and autonomic, 2A -
配列類似性
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WNK subfamily.
Contains 1 protein kinase domain. -
翻訳後修飾
O-glycosylated.
Ubiquitinated in vitro by the BCR(KLHL3) complex and in vivo by a BCR(KLHL2) complex, leading to proteasomal degradation. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 65125 Human
- Entrez Gene: 232341 Mouse
- Omim: 605232 Human
- SwissProt: Q9H4A3 Human
- SwissProt: P83741 Mouse
- Unigene: 744906 Human
- Unigene: 333349 Mouse
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別名
- Erythrocyte 65 kDa protein antibody
- HSAN2 antibody
- HSN2 antibody
see all
画像
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Sandwich ELISA - Anti-WNK1 antibody [EPR24632-107] - BSA and Azide free (Capture) (ab302753)Sandwich ELISA of ab302752 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.
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![Anti-WNK1 antibody [EPR24632-107] (BSA and Azide free (Capture)) (AB302753)](https://www.abcam.co.jp/ps/products/302/ab302753/Images/AB302753-1-Benefits-of-Recombinant-antibodies.jpg)
Anti-WNK1 antibody [EPR24632-107] (BSA and Azide free (Capture)) (AB302753)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab302753 は論文での使用が確認できていません。
