AB250940

Anti-WISP3 抗体 [EPR14769] - BSA and Azide free

Anti-WISP3 antibody [EPR14769] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal WISP3 antibody. Carrier free. Suitable for WB and reacts with Mouse, Human, Rat samples. Cited in 1 publication.

別名を表示する

WISP3, UNQ462/PRO790/PRO956, CCN6, Cellular communication network factor 6, CCN family member 6, WNT1-inducible-signaling pathway protein 3, WISP-3

2 Images

Supplier Data

Western blot - Anti-WISP3 antibody [EPR14769] - BSA and Azide free (AB250940)

This data was developed using ab187666, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-WISP3 antibody [EPR14769] - C-terminal (<a href='/products/primary-antibodies/wisp3-antibody-epr14769-c-terminal-ab187666'>ab187666</a>) at 1/1000 dilution

Lane 1:

Caco-2 cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 39 kDa

Observed band size: 45 kDa

false

Supplier Data

Western blot - Anti-WISP3 antibody [EPR14769] - BSA and Azide free (AB250940)

This data was developed using ab187666, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-WISP3 antibody [EPR14769] - C-terminal (<a href='/products/primary-antibodies/wisp3-antibody-epr14769-c-terminal-ab187666'>ab187666</a>) at 1/1000 dilution

Lane 1:

Mouse brain lysate at 10 µg

Lane 2:

Mouse heart lysate at 10 µg

Lane 3:

Rat brain lysate at 10 µg

Lane 4:

C6 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 39 kDa

Observed band size: 45 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR14769

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Mouse, Rat, Human

アプリケーション

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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製品の詳細

ab250940 is the carrier-free version of ab187666.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Constituents: PBS

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

Do Not Freeze

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

WISP3 also known as CCN6 is a protein with a mass of approximately 39 kDa. It belongs to the CCN family of proteins which are involved in diverse cellular processes. WISP3 is primarily expressed in cartilage and connective tissues such as bone and skin. This protein interacts with growth factors and other signaling molecules influencing cellular growth and maintenance. WISP3's mechanical role involves binding to extracellular matrix components which affects cell adhesion and communication.

Biological function summary

WISP3 functions as an important modulator of cartilage homeostasis and matrix production. It takes part in processes that regulate cartilage integrity and interacts with other extracellular proteins to maintain the physical properties of connective tissues. One of its roles includes modulating interactions with the insulin-like growth factor (IGF) binding proteins. These interactions support tissue structural integrity and cellular communication essential for development and repair mechanisms.

Pathways

WISP3 participates in the regulation of the Wnt signaling pathway. This pathway plays an important role in chondrogenesis and osteogenesis processes vital for bone and cartilage development. WISP3 influences the activity of key proteins within this pathway such as Wnt and Frizzled to modulate gene expression. It also interacts with proteins like beta-catenin which are pivotal to these signaling cascades therefore contributing to tissue specification and differentiation processes.

WISP3 mutations are associated with Progressive Pseudorheumatoid Dysplasia (PPD) and have connections to conditions affecting skeletal development. PPD is a disorder characterized by cartilage degeneration leading to joint pain and stiffness. WISP3's interaction with IGF-related proteins is critical in these conditions as dysregulation can lead to improper tissue development and homeostasis. Studies also link abnormal WISP3 activity to osteoarthritis where its altered expression might contribute to the disease pathology by affecting cartilage cell signaling and matrix production.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Plays a role in mitochondrial electron transport and mitochondrial respiration (PubMed : 27252383). Through its regulation of the mitochondrial function may play a role in normal postnatal skeletal growth and cartilage homeostasis (PubMed : 10471507, PubMed : 27252383).

See full target information CCN6

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Endocrine-related cancer 25:35-50 PubMed29042395

2017

Nuclear receptor profiling in prostatospheroids and castration-resistant prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zhu Wang,Dinglan Wu,Chi-Fai Ng,Jeremy Yuen-Chun Teoh,Shan Yu,Yuliang Wang,Franky L Chan

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-WISP3 抗体 [EPR14769] - BSA and Azide free

Anti-WISP3 antibody [EPR14769] - BSA and Azide free

Western blot - Anti-WISP3 antibody [EPR14769] - BSA and Azide free (AB250940)

Western blot - Anti-WISP3 antibody [EPR14769] - BSA and Azide free (AB250940)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (1)

組合せ製品

Secondary Antibodies

Proteins & Peptides

Primary Antibodies

Proteins & Peptides

Abcam product promise