Anti-wide spectrum Cytokeratin 抗体 (ab9377)
Key features and details
- Rabbit polyclonal to wide spectrum Cytokeratin
- Suitable for: IHC-P, ICC, ICC/IF, Flow Cyt, WB, IHC-Fr
- Reacts with: Mouse, Rat, Cow, Dog, Human, Pig, Cynomolgus monkey, Common marmoset
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-wide spectrum Cytokeratin antibody -
製品の詳細
Rabbit polyclonal to wide spectrum Cytokeratin -
由来種
Rabbit -
特異性
This antibody reacts with cytokeratins of MW 58, 56, 52, 60, 51, 48 and 68 kDa. -
アプリケーション
適用あり: IHC-P, ICC, ICC/IF, Flow Cyt, WB, IHC-Frmore details -
種交差性
交差種: Mouse, Rat, Cow, Dog, Human, Pig, Cynomolgus monkey, Common marmoset -
免疫原
Full length native protein (purified) corresponding to Cow wide spectrum Cytokeratin. Cytokeratin isolated from bovine muzzle epidermis.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.3
Preservative: 0.1% Sodium azide
Constituents: 10% PBS, 1% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
軽鎖の種類
unknown -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab9377の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | (28) |
Use at an assay dependent concentration. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. This antibody may be diluted to a titer of 1:75-1:200 in an ABC method.
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| ICC | (5) |
Use at an assay dependent concentration.
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| ICC/IF | (4) |
Use at an assay dependent concentration.
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| Flow Cyt |
Use at an assay dependent concentration.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB | (4) |
Use at an assay dependent concentration.
Used at a dilution of 1/1000 overnight incubation (see Abreview for further information). |
| IHC-Fr | (2) |
Use at an assay dependent concentration.
This antibody may be diluted to a titer of 1/75 - 1/200 in an ABC method. |
| 特記事項 |
|---|
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IHC-P
Use at an assay dependent concentration. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. This antibody may be diluted to a titer of 1:75-1:200 in an ABC method. |
|
ICC
Use at an assay dependent concentration. |
|
ICC/IF
Use at an assay dependent concentration. |
|
Flow Cyt
Use at an assay dependent concentration. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
Use at an assay dependent concentration. Used at a dilution of 1/1000 overnight incubation (see Abreview for further information). |
|
IHC-Fr
Use at an assay dependent concentration. This antibody may be diluted to a titer of 1/75 - 1/200 in an ABC method. |
ターゲット情報
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別名
- pan cytokeratin antibody
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-wide spectrum Cytokeratin antibody (ab9377)This image is courtesy of an Abreview submitted by Cameron Johnstone.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of 10% formalin-fixed Human transitional cell carcinoma of the kidney. Stained with ab9377 at 1/100 dilution. Secondary antibody used was goat anti-rabbit Alexa-568® at 1/500 dilution. Blocking was done with 5% serum for 1 hour at 25°C. The sample was incubated with the primary antibody for 1 hour at 25°C with 5% normal goat serum. Antigen retrieval method was heat mediated with EDTA pH 8.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-wide spectrum Cytokeratin antibody (ab9377)ab9377 staining wide spectrum Cytokeratin in formalin-fixed, paraffin-embedded Human skin tissue by Immunohistochemistry. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-wide spectrum Cytokeratin antibody (ab9377)Image is courtesy of an anonymous AbReviewab9377 staining wide spectrum Cytokeratin in Human skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with BlockAid for 1 hour at 20°C; antigen retrieval was by heat mediation in a citric buffer. Samples were incubated with primary antibody (1/500 in 1% BSA + PBS) for 16 hours at 4°C. An Alexa Fluor® 555-conjugated Goat anti-rabbit IgG (H+L) polyclonal (1/1000) was used as the secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-wide spectrum Cytokeratin antibody (ab9377)This image is courtesy of an Abreview by Carl Hobbs.ab9377 staining wide spectrum Cytokeratin in rat liver tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with formaldehyde and blocked with 2% BSA for 10 minutes at 21°C and antigen retrieval was by heat mediation in citrate buffer. The sample was incubated with primary antibody (1/500 in TBS/BSA/azide) at 21°C for 2 hours. A biotin-conjugated goat anti-rabbit polyclonal (1/300) was used as the secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-wide spectrum Cytokeratin antibody (ab9377)This image is courtesy of an Abreview by Carl Hobbs.ab9377 staining wide spectrum Cytokeratin in common marmoset lung tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with formaldehyde and blocked with 2% BSA for 10 minutes at 21°C and antigen retrieval was by heat mediation in citrate buffer. The sample was incubated with primary antibody (1/500 in TBS/BSA/azide) at 21°C for 2 hours. A biotin-conjugated goat anti-rabbit polyclonal (1/300) was used as the secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-wide spectrum Cytokeratin antibody (ab9377)Ab9377 positively staining Cytokeratin (green) in paraffin fixed mouse oesophagus at 1/250.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (174)
ab9377 は 174 報の論文で使用されています。
- Jung YR et al. Decreased SMP30 Expression Is Related With EMT in the Kidneys of Two Siberian Tigers With CKD. In Vivo 38:226-234 (2024). PubMed: 38148068
- Martinez-Ordoñez A et al. Whole-mount staining of mouse colorectal cancer organoids and fibroblast-organoid co-cultures. STAR Protoc 4:102243 (2023). PubMed: 37083323
- Dietsch B et al. Hepatic passaging of NRAS-mutant melanoma influences adhesive properties and metastatic pattern. BMC Cancer 23:436 (2023). PubMed: 37179302
- Greenman R et al. CCL24 regulates biliary inflammation and fibrosis in primary sclerosing cholangitis. JCI Insight 8:N/A (2023). PubMed: 37345655
- Porter BA et al. Glucocorticoid Receptor Activation in Lobular Breast Cancer Is Associated with Reduced Cell Proliferation and Promotion of Metastases. Cancers (Basel) 15:N/A (2023). PubMed: 37835373