Anti-WHSC2/NELF-A 抗体 (ab85852)
Key features and details
- Rabbit polyclonal to WHSC2/NELF-A
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-WHSC2/NELF-A antibody
WHSC2/NELF-A 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to WHSC2/NELF-A -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse
-
免疫原
Synthetic peptide within Human WHSC2/NELF-A aa 499-549. The exact sequence is proprietary.
Database link: Q9H3P2 -
ポジティブ･コントロール
- WB: HeLa whole cell lysate. IP: HeLa whole cell lysate. IHC-P: Human Placenta tissue.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab85852 was affinity purified using an epitope specific to WHSC2/NELF-A immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab85852の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 60 kDa.
|
|
| IP |
Use at 2-5 µg/mg of lysate.
|
|
| IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 特記事項 |
|---|
|
WB
1/2000 - 1/10000. Predicted molecular weight: 60 kDa. |
|
IP
Use at 2-5 µg/mg of lysate. |
|
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
-
機能
Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. Probably required to interact with the RNA polymerase II complex. -
組織特異性
Ubiquitous. Expressed in heart, brain, placenta, liver, skeletal muscle, kidney and pancreas. Expressed at lower level in adult lung. Expressed in fetal brain, lung, liver and kidney. -
配列類似性
Belongs to the NELF-A family.
Contains 1 HDAg-like domain. -
ドメイン
The HDAg-like domain is essential for transcriptional repression, and mediates the interaction with the RNA polymerase II complex. -
細胞内局在
Nucleus. - Information by UniProt
-
参照データベース
- Entrez Gene: 7469 Human
- Entrez Gene: 24116 Mouse
- Omim: 606026 Human
- SwissProt: Q9H3P2 Human
- SwissProt: Q8BG30 Mouse
- Unigene: 21771 Human
- Unigene: 332320 Mouse
-
別名
- FLJ10442 antibody
- FLJ25112 antibody
- Negative elongation factor A antibody
see all
画像
-
Western blot - Anti-WHSC2/NELF-A antibody (ab85852)All lanes : Anti-WHSC2/NELF-A antibody (ab85852) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 60 kDa
Observed band size: 60 kDa
Exposure time: 3 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WHSC2/NELF-A antibody (ab85852)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue labelling WHSC2 with ab85852 at 1/1000 (1µg/ml). Detection: DAB. -
Immunoprecipitation - Anti-WHSC2/NELF-A antibody (ab85852)WHSC2/NELF-A was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg/IP; 20% of IP loaded/lane) using ab85852 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab85852, at 1 µg/ml.
Lane 1: ab85852 IP in HeLa whole cell lysate.
Lane 2: Control IgG.Detection: Chemiluminescence with exposure times of 3 minutes
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (2)
ab85852 は 2 報の論文で使用されています。
- Zhu J et al. Regulation of Pol II Pausing Is Involved in Daily Gene Transcription in the Mouse Liver. J Biol Rhythms 33:350-362 (2018). PubMed: 29845885
- Awwad SW et al. NELF-E is recruited to DNA double-strand break sites to promote transcriptional repression and repair. EMBO Rep 18:745-764 (2017). PubMed: 28336775