AB225625

Anti-WHSC1/NSD2 抗体

Name: Anti-WHSC1/NSD2 抗体 (ab225625) | Abcam
Brand: Abcam Limited
SKU: AB225625
Price: 73000 JPY
Availability: InStock

Anti-WHSC1/NSD2 antibody

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(2 Publications)

Rabbit Polyclonal WHSC1/NSD2 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human NSD2 aa 500-600.

別名を表示する

KIAA1090, MMSET, TRX5, WHSC1, NSD2, Histone-lysine N-methyltransferase NSD2, Multiple myeloma SET domain-containing protein, Nuclear SET domain-containing protein 2, Protein trithorax-5, Wolf-Hirschhorn syndrome candidate 1 protein

1 Images

Supplier Data

Western blot - Anti-WHSC1/NSD2 antibody (AB225625)

All lanes:

Western blot - Anti-WHSC1/NSD2 antibody (ab225625) at 0.04 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg

Lane 3:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg

Predicted band size: 152 kDa

true

Exposure time: 30s

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

applications

免疫原

Synthetic Peptide within Human NSD2 aa 500-600. The exact immunogen used to generate this antibody is proprietary information.

O96028

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

精製に関する特記事項

ab225625 was affinity purified using an epitope specific to WHSC1/NSD2 immobilized on solid support.

バッファー組成

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

+4°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

WHSC1 also known as NSD2 is a histone methyltransferase enzyme involved in the methylation of lysine 36 on histone H3. This enzyme facilitates the addition of a methyl group which affects chromatin structure and gene expression. NSD2 is also called WHSC1L1 or Wolf-Hirschhorn syndrome candidate 1-like 1. It has a molecular weight around 153 kDa. Researchers observe the expression of WHSC1/NSD2 predominantly in the nucleus but it is present in various tissues including lymphoid organs and certain types of cancer cells.

Biological function summary

WHSC1/NSD2 functions as an epigenetic regulator modifying histones to modulate transcriptional activity. It exists as part of a larger protein complex that includes several chromatin-associated proteins. Its role in histone methylation influences gene expression impacting cell differentiation survival and proliferation. Through controlling gene transcription WHSC1/NSD2 plays a significant role in development and cellular responses to environmental signals.

Pathways

WHSC1/NSD2 participates in key biological processes such as the cell cycle and DNA damage response pathways. It influences histone modification pathways by interacting with other proteins like SETD2 and H3K36me3 which are important for maintaining genomic integrity. In the DNA damage response NSD2 works together with proteins such as ATM coordinating repair mechanisms and cell cycle progression.

Alterations in WHSC1/NSD2 expression or function associate with multiple myeloma and Wolf-Hirschhorn syndrome. In multiple myeloma overexpression or mutations of WHSC1/NSD2 lead to altered gene expression profiles and promote cancer cell proliferation. Within this context NSD2 often interacts with MMSET and FGFR3 proteins contributing to oncogenic transformation. Additionally mutations in WHSC1/NSD2 link to Wolf-Hirschhorn syndrome a genetic condition characterized by growth delay and intellectual disability.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Histone methyltransferase which specifically dimethylates nucleosomal histone H3 at 'Lys-36' (H3K36me2) (PubMed : 19808676, PubMed : 22099308, PubMed : 27571355, PubMed : 29728617, PubMed : 33941880). Also monomethylates nucleosomal histone H3 at 'Lys-36' (H3K36me) in vitro (PubMed : 22099308). Does not trimethylate nucleosomal histone H3 at 'Lys-36' (H3K36me3) (PubMed : 22099308). However, specifically trimethylates histone H3 at 'Lys-36' (H3K36me3) at euchromatic regions in embryonic stem (ES) cells (By similarity). By methylating histone H3 at 'Lys-36', involved in the regulation of gene transcription during various biological processes (PubMed : 16115125, PubMed : 22099308, PubMed : 29728617). In ES cells, associates with developmental transcription factors such as SALL1 and represses inappropriate gene transcription mediated by histone deacetylation (By similarity). During heart development, associates with transcription factor NKX2-5 to repress transcription of NKX2-5 target genes (By similarity). Plays an essential role in adipogenesis, by regulating expression of genes involved in pre-adipocyte differentiation (PubMed : 29728617). During T-cell receptor (TCR) and CD28-mediated T-cell activation, promotes the transcription of transcription factor BCL6 which is required for follicular helper T (Tfh) cell differentiation (By similarity). During B-cell development, required for the generation of the B1 lineage (By similarity). During B2 cell activation, may contribute to the control of isotype class switch recombination (CRS), splenic germinal center formation, and the humoral immune response (By similarity). Plays a role in class switch recombination of the immunoglobulin heavy chain (IgH) locus during B-cell activation (By similarity). By regulating the methylation of histone H3 at 'Lys-36' and histone H4 at 'Lys-20' at the IgH locus, involved in TP53BP1 recruitment to the IgH switch region and promotes the transcription of IgA (By similarity).. Isoform 1. Histone methyltransferase which specifically dimethylates nucleosomal histone H3 at 'Lys-36' (H3K36me2).. Isoform 4. Histone methyltransferase which specifically dimethylates nucleosomal histone H3 at 'Lys-36' (H3K36me2) (PubMed : 22099308). Methylation of histone H3 at 'Lys-27' is controversial (PubMed : 18172012, PubMed : 22099308). Mono-, di- or tri-methylates histone H3 at 'Lys-27' (H3K27me, H3K27me2 and H3K27me3) (PubMed : 18172012). Does not methylate histone H3 at 'Lys-27' (PubMed : 22099308). May act as a transcription regulator that binds DNA and suppresses IL5 transcription through HDAC recruitment (PubMed : 11152655, PubMed : 18172012).

See full target information NSD2

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Cell transplantation 30:963689720983786 PubMed33588586

2021

Circ SMARCA5 Inhibited Tumor Metastasis by Interacting with SND1 and Downregulating the YWHAB Gene in Cervical Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xia Zhang,Qing Zhang,Ke Zhang,Fang Wang,Xiaogai Qiao,Jinquan Cui

Journal for immunotherapy of cancer 9: PubMed33589522

2021

WHSC1/NSD2 regulates immune infiltration in prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Muzamil Y Want,Takemasa Tsuji,Prashant K Singh,James L Thorne,Junko Matsuzaki,Ellen Karasik,Bryan Gillard,Eduardo Cortes Gomez,Richard C Koya,Amit Lugade,Kunle Odunsi,Sebastiano Battaglia

View all publications

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Abcam product promise

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Anti-WHSC1/NSD2 抗体

Anti-WHSC1/NSD2 antibody

Western blot - Anti-WHSC1/NSD2 antibody (AB225625)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (2)

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Abcam product promise