Anti-Werner's syndrome helicase WRN 抗体 (ab17987)

Thank you for your enquiry. As you know, the molecular weight of BSA is expected to be 67kDa, which is lower that the 75-105kDa band area that you observed. In addition, I have confirmed with the originator of this product that it does not contain BSA. I am sorry that I do not know what the extra band is, or whether it is non-specific. I am sorry I can't be of more assistance in this occasion. Should you require any further information, please do not hesitate to contact me.

Thank you for your enquiry. I can confirm that this product is supposed to detect an approximately 200 kDa band and there are no BSA included in the product. In your previous submitted Abreview, you mentioned that you were able to obtain a specific 190kDa band and a non-specific 80kDa band. In your recent experiment, did you also managed to get a 190kDa band together with this 75~105kDa band? I am unsure what the 75~105kDa band may be but I would assume that it would a non-specific band. If you would like some technical support I enclose a link to a questionnaire which will help you put your protocol information together very easily so we can look into the details of the experiment and provide some help. https://www.abcam.com/index.html?section=western&pageconfig=technical&intAbID=17987&mode=questionaire The BL1308 and BL1309 you are referring to is actually ab17987 and ab17988, respectively. The difference between these two can be found in the immunogen sequences. The epitope recognized by ab17987 maps to a region between residues 400 and 450 if human Werner Syndrome Helicase. While, the epitope recognized by ab17988 is a region between residues 1400 and the C-terminus (residue 1432) of human Werner Syndrome Helicase. I hope the information above will be useful to you. Should you require any further information, please do not hesitate to contact me.

Thank you for your enquiry. You ask an interesting question. I have been in discussion with the lab and they have referred me to the fact that the resolution of proteins by SDS-PAGE does not define molecular weight. It defines the relative mobility of the protein in SDS-PAGE; relative to the molecular weight standards. The molecular weight standards vary from lab to lab. One can take several different sets of molecular weight markers from the same or different sources and run them on a given gel. If done, one will see that there are discrepancies in where a given size supposedly runs on the gel. There are two sets of molecular weight markers on the market that lend themselves well to this example. One has a marker for 148 kDa, the other a marker for 180 kDa. If run side-by-side one will find that the 148 kDa marker runs higher (larger) than the 180 kDa marker. Another point is that the gel and buffer system used has a significant influence on the mobility of the molecular weight markers. The specificity of the antibodies for Werner's syndrome helicase WRN have been tested extensively. They have been shown to react against the target by IP and recognised using another of our antibodies (ab200) by subsequent western blotting. Furthermore there is no staining in human fibroblast WRN knock-out cells. The antibody works well by immunofluoresence on WRN +/+ cells and is negative on WRN -/- cells. The immunoprecipitation of this protein is at least partially blocked by the blocking peptides for both of the antibodies. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.