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AB17987

Anti-Werner's syndrome helicase WRN 抗体

Anti-Werner's syndrome helicase WRN antibody

4

(2 Reviews)

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(1 Publication)

Rabbit Polyclonal Werner's syndrome helicase WRN antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication.

別名を表示する

RECQ3, RECQL2, WRN, Bifunctional 3'-5' exonuclease/ATP-dependent helicase WRN, RecQ protein-like 2, Werner syndrome protein

2 Images
Immunoprecipitation - Anti-Werner's syndrome helicase WRN antibody (AB17987)
  • IP

Supplier Data

Immunoprecipitation - Anti-Werner's syndrome helicase WRN antibody (AB17987)

Werner's syndrome helicase WRN was immunoprecipitated from HeLa whole cell lysate with 3 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab17987 at 1 μg/ml dilution.

Lane 1 : ab17987 IP in HeLa whole cell lysate.

Lane 2 : Control IgG IP in HeLa whole cell lysate.

All lanes:

Immunoprecipitation - Anti-Werner's syndrome helicase WRN antibody (ab17987)

Predicted band size: 162 kDa

false

Western blot - Anti-Werner's syndrome helicase WRN antibody (AB17987)
  • WB

Supplier Data

Western blot - Anti-Werner's syndrome helicase WRN antibody (AB17987)

Detection : Chemiluminescence with exposure time of 30 second

All lanes:

Western blot - Anti-Werner's syndrome helicase WRN antibody (ab17987) at 1/5000 dilution

Lane 1:

Hela Whole cell lysate at 50 µg

Lane 2:

Hela Whole cell lysate at 15 µg

Lane 3:

Hela Whole cell lysate at 5 µg

Predicted band size: 162 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

IP, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2.00000-5.00000 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000.00000 - 1/25000.00000", "WB-species-notes": "<p></p>" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
バッファー組成
pH: 7 - 8 Preservative: 0.1% Sodium azide Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Werner's syndrome helicase also known as WRN plays a mechanical role in DNA replication and repair. WRN is a 160 kDa protein containing both helicase and exonuclease activities making it unique among RecQ helicases. It unwinds DNA duplexes and resolves complex DNA structures that can arise during replication and repair processes. WRN is expressed in the nucleus of various cell types including fibroblasts where it maintains genome stability. This helicase is critical in processing DNA intermediates to prevent genomic instability.
Biological function summary

WRN functions as a part of larger protein complexes that participate in DNA damage response and repair. It interacts with several proteins involved in the homologous recombination and base excision repair pathways. WRN's unique enzymatic activities enable it to stabilize and process DNA ends during replication fork collapse and double-strand break repair. By operating in concert with proteins such as RAD51 and replication protein A (RPA) WRN ensures accurate replication and repair preventing mutation accumulation.

Pathways

WRN plays a central role in the maintenance of genomic integrity through its involvement in DNA repair pathways and the aging process. It is integral to the process of homologous recombination where it works closely with the MRE11-RAD50-NBS1 (MRN) complex. Additionally WRN has a function in the DNA damage checkpoint pathway collaborating with proteins like ATM and ATR which help to sense damaged DNA and initiate repair processes. These pathways are important for maintaining DNA integrity and preventing cellular senescence.

WRN is closely linked to Werner syndrome and cancer. Mutations in the WRN gene lead to Werner syndrome a rare disorder characterized by premature aging and cancer predisposition. WRN's interaction with tumor suppressor proteins such as p53 connects it to pathways that can contribute to tumorigenesis when dysregulated. Understanding WRN's function and its role in these diseases provides insight into mechanisms of aging and cancer development offering potential therapeutic targets.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Multifunctional enzyme that has magnesium and ATP-dependent 3'-5' DNA-helicase activity on partially duplex substrates (PubMed : 9224595, PubMed : 9288107, PubMed : 9611231). Also has 3'->5' exonuclease activity towards double-stranded (ds)DNA with a 5'-overhang (PubMed : 11863428). Has no nuclease activity towards single-stranded (ss)DNA or blunt-ended dsDNA (PubMed : 11863428). Helicase activity is most efficient with (d)ATP, but (d)CTP will substitute with reduced efficiency; strand displacement is enhanced by single-strand binding-protein (heterotrimeric replication protein A complex, RPA1, RPA2, RPA3) (PubMed : 9611231). Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A (By similarity). Plays a role in double-strand break repair after gamma-irradiation (PubMed : 9224595, PubMed : 9288107, PubMed : 9611231). Unwinds some G-quadruplex DNA (d(CGG)n tracts); unwinding seems to occur in both 5'-3' and 3'-5' direction and requires a short single-stranded tail (PubMed : 10212265). d(CGG)n tracts have a propensity to assemble into tetraplex structures; other G-rich substrates from a telomeric or IgG switch sequence are not unwound (PubMed : 10212265). Depletion leads to chromosomal breaks and genome instability (PubMed : 33199508).
See full target information WRN
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of cell science 119:5137-46 PubMed17118963

2006

Werner syndrome protein participates in a complex with RAD51, RAD54, RAD54B and ATR in response to ICL-induced replication arrest.

Applications

IP, WB

Species

Human, Human

Marit Otterlei,Per Bruheim,Byungchan Ahn,Wendy Bussen,Parimal Karmakar,Kathy Baynton,Vilhelm A Bohr
View all publications
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保証に関する詳細については利用規約をご確認ください。
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