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リコンビナントRabMAb

Anti-WDR77 抗体 [EPR10708(B)] (ab154190)

Reviews (2) Submit a question References (5)
Western blot - Anti-WDR77 antibody [EPR10708(B)] (ab154190)
  • Immunocytochemistry/ Immunofluorescence - Anti-WDR77 antibody [EPR10708(B)] (ab154190)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR77 antibody [EPR10708(B)] (ab154190)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR77 antibody [EPR10708(B)] (ab154190)
  • Flow Cytometry (Intracellular) - Anti-WDR77 antibody [EPR10708(B)] (ab154190)
  • Anti-WDR77 antibody [EPR10708(B)] (ab154190)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10708(B)] to WDR77
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IP
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-WDR77 antibody [EPR10708(B)]
    WDR77 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR10708(B)] to WDR77

  • 由来種

    Rabbit

  • 特異性

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • アプリケーション

    適用あり: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IPmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • IHC-P: Human breast cancer and human testis tissue; ICC/IF: 293T cells; Flow Cyt (intra): HeLa cells. WB: HeLa, HEK-293 whole cell lysate. Mouse kidney and spleen lysate and Rat lysate.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab154190の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/30.

For unpurified use at 1/100 - 1/10000.  ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB (1)
1/1000 - 1/10000. Predicted molecular weight: 37 kDa.
IHC-P
1/6000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/50 - 1/100.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
ICC/IF (1)
1/50 - 1/250.
IP
Use at an assay dependent concentration.
特記事項
Flow Cyt (Intra)
1/30.

For unpurified use at 1/100 - 1/10000.  ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Predicted molecular weight: 37 kDa.
IHC-P
1/6000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/50 - 1/100.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
ICC/IF
1/50 - 1/250.
IP
Use at an assay dependent concentration.

ターゲット情報

  • 機能

    Non-catalytic component of the 20S PRMT5-containing methyltransferase complex, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins. This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles. Might play a role in transcription regulation. The 20S PRMT5-containing methyltransferase complex also methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage.

  • 組織特異性

    Highly expressed in heart, skeletal muscle, spleen, testis, uterus, prostate and thymus. In testis, expressed in germ cells and Leydig cells, but not in peritubular myocytes, nor in Sertoli cells. Expressed in prostate cancers, in seminomas and in Leydig cell tumors.

  • 配列類似性

    Contains 5 WD repeats.

  • 発生段階

    Expressed in Leydig cells during fetal testicular development, especially during the second semester. Germ cells expression is detected as early as 10 weeks of gestation.

  • 細胞内局在

    Nucleus. Cytoplasm. Nuclear in Leydig cells and cytoplasmic in germ cells during fetal testicular development. In adult testis, predominantly nuclear. Subcellular location varies from nuclear to cytoplasmic in various tumors.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • 2610312E17Rik antibody
    • Androgen receptor cofactor p44 antibody
    • C79984 antibody
    • HKMT1069 antibody
    • MEP 50 antibody
    • MEP-50 antibody
    • MEP50 antibody
    • MEP50_HUMAN antibody
    • Methylosome protein 50 antibody
    • MGC2722 antibody
    • Nbla10071 antibody
    • p44 antibody
    • p44/Mep50 antibody
    • RGD1310479 antibody
    • RP11 552M11.3 antibody
    • WD repeat containing protein 77 antibody
    • WD repeat domain 77 antibody
    • WD repeat-containing protein 77 antibody
    • WDR77 antibody
    see all

画像

  • Western blot - Anti-WDR77 antibody [EPR10708(B)] (ab154190)
    Western blot - Anti-WDR77 antibody [EPR10708(B)] (ab154190)
    All lanes : Anti-WDR77 antibody [EPR10708(B)] (ab154190) at 1/1000 dilution (Purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate
    Lane 3 : Mouse spleen lysate
    Lane 4 : Mouse kidney lysate
    Lane 5 : Rat kidney lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 37 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-WDR77 antibody [EPR10708(B)] (ab154190)
    Immunocytochemistry/ Immunofluorescence - Anti-WDR77 antibody [EPR10708(B)] (ab154190)

    Immunocytochemistry analysis of 293T (Human embryonic kidney epithelial cell) cells labeling WDR77 with purified ab154190 at 1/50 dilution (5.58 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR77 antibody [EPR10708(B)] (ab154190)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR77 antibody [EPR10708(B)] (ab154190)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue sections labeling WDR77 with purified ab154190 at 1/6000 dilution (0.05 µg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR77 antibody [EPR10708(B)] (ab154190)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR77 antibody [EPR10708(B)] (ab154190)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast cancer tissue sections labeling WDR77 with purified ab154190 at 1/6000 dilution (0.05 µg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  • Flow Cytometry (Intracellular) - Anti-WDR77 antibody [EPR10708(B)] (ab154190)
    Flow Cytometry (Intracellular) - Anti-WDR77 antibody [EPR10708(B)] (ab154190)

    Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling WDR77 with purified ab154190 at 1/30 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).

     

  • Anti-WDR77 antibody [EPR10708(B)] (ab154190)
    Anti-WDR77 antibody [EPR10708(B)] (ab154190)

参考文献 (5)

ab154190 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab154190 は 5 報の論文で使用されています。

  • Li J  et al. WDR77 inhibits prion-like aggregation of MAVS to limit antiviral innate immune response. Nat Commun 14:4824 (2023). PubMed: 37563140
  • Chen M  et al. PRMT5 regulates ovarian follicle development by facilitating Wt1 translation. Elife 10:N/A (2021). PubMed: 34448450
  • Lu W  et al. The N-terminal sequence of murine PRMT5 variant 2 is required for Hsp70 interaction and CHIP ligase-mediated degradation. Biochem Biophys Res Commun 514:1185-1191 (2019). PubMed: 31103260
  • Qi H  et al. Sirtuin 7-mediated deacetylation of WD repeat domain 77 (WDR77) suppresses cancer cell growth by reducing WDR77/PRMT5 transmethylase complex activity. J Biol Chem 293:17769-17779 (2018). PubMed: 30282801
  • Lubyova B  et al. PRMT5: A novel regulator of Hepatitis B virus replication and an arginine methylase of HBV core. PLoS One 12:e0186982 (2017). WB ; Human . PubMed: 29065155

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1-2 of 2 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-WDR77 antibody [EPR10708(B)]

 Good
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (U2OS cells)
Specification
U2OS cells
Permeabilization
Yes - PBS+ 0.2% Triton-X 100, 10 minutes at RT
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Melanie Blasius

Verified customer

投稿 Jun 28 2013

Western blot

 Excellent
Abreviews
abreview image
Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (NuPAGE® Novex® 3-8% Tris-Acetate Gel 1.0 mm)
Sample
Human Cell lysate - whole cell (U2OS cells)
Specification
U2OS cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Melanie Blasius

Verified customer

投稿 May 20 2013

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