Anti-WDR5 抗体 (ab245479)
Key features and details
- Rabbit polyclonal to WDR5
- Suitable for: IP, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-WDR5 antibody
WDR5 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to WDR5 -
由来種
Rabbit -
アプリケーション
適用あり: IP, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow
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免疫原
Synthetic peptide within Human WDR5 aa 1-50. The exact sequence is proprietary. NP_060058.1
Database link: P61964 -
ポジティブ･コントロール
- IP: WDR5 IP in HeLa whole cell lysate. IHC-P: Human ovarian carcinoma tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7-8 -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab245479 was affinity purified using an epitope specific to WDR5 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab245479の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP |
Use at 2-10 µg/mg of lysate.
|
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| IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
|
IP
Use at 2-10 µg/mg of lysate. |
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IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Contributes to histone modification. May position the N-terminus of histone H3 for efficient trimethylation at 'Lys-4'. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues. May regulate osteoblasts differentiation. -
配列類似性
Belongs to the WD repeat WDR5/wds family.
Contains 7 WD repeats. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 100125836 Cow
- Entrez Gene: 11091 Human
- Entrez Gene: 140858 Mouse
- Entrez Gene: 362093 Rat
- Omim: 609012 Human
- SwissProt: Q2KIG2 Cow
- SwissProt: P61964 Human
- SwissProt: P61965 Mouse
see all -
別名
- 2410008O07Rik antibody
- AA408785 antibody
- AA960360 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR5 antibody (ab245479)
Formalin-fixed paraffin-embedded human ovarian carcinoma tissue stained for WDR5 using ab245479 at 1/1000 dilution in immunohistochemical analysis. DAB staining.
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Immunoprecipitation - Anti-WDR5 antibody (ab245479)WDR5 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) using ab245479 at 6 µg per reaction. Western blot was perfromed on the immunoprecipitate using another anti-WDR5 antibody at 1 µg/ml.
Lane 1: ab245479 IP in HeLa whole cell lysate.
Lane 2: Contol IgG IP in HeLa whole cell lysate.Detection: Chemiluminescence with an exposure time of 3 minutes.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab245479 は 1 報の論文で使用されています。
- Zhang Y et al. LncRNA-CCDC144NL-AS1 Promotes the Development of Hepatocellular Carcinoma by Inducing WDR5 Expression via Sponging miR-940. J Hepatocell Carcinoma 8:333-348 (2021). PubMed: 33977095