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  1. Link

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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Methylation
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Anti-WDR5 抗体 [2C2] (ab56919)

  • Datasheet
Reviews (2)Q&A (3)References (35)

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Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Western blot - Anti-WDR5 antibody [2C2] (ab56919)
  • Flow Cytometry - Anti-WDR5 antibody [2C2] (ab56919)
  • Immunocytochemistry/ Immunofluorescence - Anti-WDR5 antibody [2C2] (ab56919)
  • Western blot - Anti-WDR5 antibody [2C2] (ab56919)

Key features and details

  • Mouse monoclonal [2C2] to WDR5
  • Suitable for: WB, ICC/IF, ChIP, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG2b

こちらの製品もご検討ください

一次抗体
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Anti-BRG1 antibody [EPNCIR111A] (ab110641)
ChIP
Methylated DNA Immunoprecipitation (MeDIP) ChIP Kit (ab117135)
二次抗体
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)

関連製品

製品の概要

  • 製品名

    Anti-WDR5 antibody [2C2]
    WDR5 一次抗体 製品一覧
  • 製品の詳細

    Mouse monoclonal [2C2] to WDR5
  • 由来種

    Mouse
  • アプリケーション

    適用あり: WB, ICC/IF, ChIP, Flow Cytmore details
  • 種交差性

    交差種: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 16-121 of Human WDR5

  • 特記事項

    This product was changed from ascites to tissue culture supernatant on 24/1/19. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7.4
  • Concentration information loading...
  • 精製度

    Tissue culture supernatant
  • 特記事項(精製)

    Purified from TCS.
  • ポリ/モノ

    モノクローナル
  • クローン名

    2C2
  • アイソタイプ

    IgG2b
  • 軽鎖の種類

    kappa
  • 研究分野

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Methylation
    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • Methyl Lysine
    • Epigenetics and Nuclear Signaling
    • Chromatin Remodeling
    • Trithorax

関連製品

  • ChIP Related Products

    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Isotype control

    • Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab170192)
  • Recombinant Protein

    • Recombinant Human WDR5 protein (ab98079)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab56919の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (1)
Use at an assay dependent concentration. Predicted molecular weight: 36 kDa.
ICC/IF
Use at an assay dependent concentration.
ChIP
Use at an assay dependent concentration. PubMed: 20864036
Flow Cyt
Use at an assay dependent concentration.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 36 kDa.
ICC/IF
Use at an assay dependent concentration.
ChIP
Use at an assay dependent concentration. PubMed: 20864036
Flow Cyt
Use at an assay dependent concentration.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能

    Contributes to histone modification. May position the N-terminus of histone H3 for efficient trimethylation at 'Lys-4'. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues. May regulate osteoblasts differentiation.
  • 配列類似性

    Belongs to the WD repeat WDR5/wds family.
    Contains 7 WD repeats.
  • 細胞内局在

    Nucleus.
  • Target information above from: UniProt accession P61964 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 11091 Human
    • Omim: 609012 Human
    • SwissProt: P61964 Human
    • Unigene: 397638 Human
    • 別名

      • 2410008O07Rik antibody
      • AA408785 antibody
      • AA960360 antibody
      • BIG 3 antibody
      • Big antibody
      • BIG3 antibody
      • BMP2 induced 3 kb gene protein antibody
      • BMP2 induced gene, 3-KB antibody
      • BMP2-induced 3-kb gene protein antibody
      • MGC114572 antibody
      • OTTHUMP00000162494 antibody
      • SWD 3 antibody
      • SWD3 antibody
      • SWD3 Set1c WD40 repeat protein homolog antibody
      • WD repeat containing protein 5 antibody
      • WD repeat domain 5 antibody
      • WD repeat protein 5 antibody
      • WD repeat-containing protein 5 antibody
      • WD repeat-containing protein BIG-3 antibody
      • WDR 5 antibody
      • Wdr5 antibody
      • WDR5_HUMAN antibody
      see all

    画像

    • Western blot - Anti-WDR5 antibody [2C2] (ab56919)
      Western blot - Anti-WDR5 antibody [2C2] (ab56919)

      WDR5 antibody (ab56919) at 1μg/lane + Hs 181 S cell lysate at 25μg/lane.

      This image was generated using the ascites version of the product.

    • Flow Cytometry - Anti-WDR5 antibody [2C2] (ab56919)
      Flow Cytometry - Anti-WDR5 antibody [2C2] (ab56919)

      Overlay histogram showing HeLA cells stained with ab56919 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56919, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.

      This image was generated using the ascites version of the product.

    • Immunocytochemistry/ Immunofluorescence - Anti-WDR5 antibody [2C2] (ab56919)
      Immunocytochemistry/ Immunofluorescence - Anti-WDR5 antibody [2C2] (ab56919)

      HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for WDR5 (green) using ab56919 at 10 µg/ml in ICC/IF. 

      This image was generated using the ascites version of the product.

    • Western blot - Anti-WDR5 antibody [2C2] (ab56919)
      Western blot - Anti-WDR5 antibody [2C2] (ab56919)
      Anti-WDR5 antibody [2C2] (ab56919) at 1 µg/ml + Hs 181.Tes cell lysate

      Predicted band size: 36 kDa



      This image was generated using the ascites version of the product.

    プロトコール

    • Flow cytometry protocols
    • Western blot protocols

    Click here to view the general protocols

    データシートおよび資料

    • Datasheet download

      Download

    参考文献 (35)

    ab56919 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab56919 は 35 報の論文で使用されています。

    • Zhou T  et al. Lupus enhancer risk variant causes dysregulation of IRF8 through cooperative lncRNA and DNA methylation machinery. Nat Commun 13:1855 (2022). PubMed: 35388006
    • Zhang C  et al. Histone methyltransferase MLL1 drives renal tubular cell apoptosis by p53-dependent repression of E-cadherin during cisplatin-induced acute kidney injury. Cell Death Dis 13:770 (2022). PubMed: 36068197
    • Ka M  et al. WDR5-HOTTIP Histone Modifying Complex Regulates Neural Migration and Dendrite Polarity of Pyramidal Neurons via Reelin Signaling. Mol Neurobiol 59:5104-5120 (2022). PubMed: 35672601
    • Fischer V  et al. The related coactivator complexes SAGA and ATAC control embryonic stem cell self-renewal through acetyltransferase-independent mechanisms. Cell Rep 36:109598 (2021). PubMed: 34433046
    • Yang B  et al. Localization of the WD repeat-containing protein 5 to the Virion Assembly Compartment Facilitates Human Cytomegalovirus Assembly. J Virol 95:N/A (2021). PubMed: 33504601
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-5 of 5 Abreviews or Q&A

    IHC - Wholemount abreview for Anti-WDR5 antibody [2C2]

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    IHC - Wholemount
    Sample
    Zebrafish Embryo (3 dpf)
    Specification
    3 dpf
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Dec 14 2021

    Western blot abreview for Anti-WDR5 antibody [2C2]

    Excellent
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Brain)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    40 µg
    Specification
    Brain
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Aug 05 2015

    Question

    I have attached the reference where the authors have used anti WDR5 antibody for probing WDR5 in HCT116 lysate. For western blot the authors have used antiWDR5 (ab22512).It would be great if you can send me this particular antibody as replacement.

    Read More

    Abcam community

    Verified customer

    Asked on Oct 29 2012

    Answer

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab22512.

    To check the status of the order please contact our Customer Service team and reference this number.

    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

    I wish you the best of luck with your research.

    Read More

    Abcam Scientific Support

    Answered on Oct 29 2012

    Question

    Hi,

    Thanks for your email! I would very much like to test ab56919. I'll wait to order it until I receive the discount code.

    Thanks so much!

    Read More

    Abcam community

    Verified customer

    Asked on Jan 26 2012

    Answer

    DISCOUNT CODE: ************
    Expiration date: ***********

    I am very pleased to hear you would like to accept our offer and test ab56919 in mouse samples. This code will give you 1 freePRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for mouse samples and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

    Abcam Scientific Support

    Answered on Jan 26 2012

    Question

    I was wondering if there was any chance your WDR5 antibody (ab56919) might also react with mouse in addition to human. Any information would be greatly appreciated!

    Thanks so much,

    Read More

    Abcam community

    Verified customer

    Asked on Jan 26 2012

    Answer

    Thank you very much for your interest in ab56919.

    To our knowledge, ab56919 has not been tested in mouse samples however based on sequence homology, I believe there isa very goodchance that it will work. Therefore, I can offer a discount off a future purchase if you buy ab56919 now, test it in mouse samples and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free PRIMARY ANTIBODY.

    If you are interested in this offer, please follow these steps:

    1. Reply to this e-mail to let me know that you would like to proceed and test ab56919 in mouse. I will then send a discount code. This code must be issued before purchasing ab56919 so please wait for my reply before ordering.

    2. Purchase ab56919either by phone, fax, or online (www.abcam.com).

    3. Test it in mouse.

    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

    5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue.

    We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab56919 turns out to be unsuitable for mouse, you will still receive the discount on your next purchase after your Abreview has been submitted.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

    Read More

    Abcam Scientific Support

    Answered on Jan 26 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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