Anti-WASP/Wiskott-Aldrich syndrome protein (phospho Y290) 抗体 (ab59278)
Key features and details
- Rabbit polyclonal to WASP/Wiskott-Aldrich syndrome protein (phospho Y290)
- Suitable for: ELISA, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-WASP/Wiskott-Aldrich syndrome protein (phospho Y290) antibody
WASP/Wiskott-Aldrich syndrome protein 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to WASP/Wiskott-Aldrich syndrome protein (phospho Y290) -
由来種
Rabbit -
アプリケーション
適用あり: ELISA, WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse
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免疫原
Synthetic peptide corresponding to Human WASP/Wiskott-Aldrich syndrome protein aa 250-350 (phospho Y290).
Database link: P42768 -
ポジティブ･コントロール
- WB: Extracts from HepG2 cells. IHC-P: Human brain tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab59278の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
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| WB |
1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 53 kDa).
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| IHC-P |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
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ELISA
Use at an assay dependent concentration. |
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WB
1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 53 kDa). |
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IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
Effector protein for Rho-type GTPases, providing a link with the Arp2/3 complex that regulates the structure and dynamics of the actin cytoskeleton. Important for efficient actin polymerization. Possible regulator of lymphocyte and platelet function. -
組織特異性
Expressed predominantly in the thymus. Also found, to a much lesser extent, in the spleen. -
関連疾患
Defects in WAS are the cause of Wiskott-Aldrich syndrome (WAS) [MIM:301000]; also known as eczema-thrombocytopenia-immunodeficiency syndrome. WAS is an X-linked recessive immunodeficiency characterized by eczema, thrombocytopenia, recurrent infections, and bloody diarrhea. Death usually occurs before age 10.
Defects in WAS are the cause of thrombocytopenia type 1 (THC1) [MIM:313900]. Thrombocytopenia is defined by a decrease in the number of platelets in circulating blood, resulting in the potential for increased bleeding and decreased ability for clotting.
Defects in WAS are a cause of neutropenia severe congenital X-linked (XLN) [MIM:300299]. XLN is an immunodeficiency syndrome characterized by recurrent major bacterial infections, severe congenital neutropenia, and monocytopenia. -
配列類似性
Contains 1 CRIB domain.
Contains 1 WH1 domain.
Contains 1 WH2 domain. -
ドメイン
The WH1 (Wasp homology 1) domain may bind a Pro-rich ligand.
The CRIB (Cdc42/Rac-interactive-binding) region binds to the C-terminal WH2 domain in the autoinhibited state of the protein. Binding of Rho-type GTPases to the CRIB induces a conformation change and leads to activation. -
細胞内局在
Cytoplasm > cytoskeleton. - Information by UniProt
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参照データベース
- Entrez Gene: 7454 Human
- Entrez Gene: 22376 Mouse
- Omim: 300392 Human
- SwissProt: P42768 Human
- SwissProt: P70315 Mouse
- Unigene: 2157 Human
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別名
- Eczema thrombocytopenia antibody
- IMD2 antibody
- SCNX antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WASP/Wiskott-Aldrich syndrome protein (phospho Y290) antibody (ab59278)Immunohistochemical analysis of paraffin-embedded human brain tissue using ab59278 at a dilution of 1/50.
Left image: no immunising peptide.
Right hand image: with immunising peptide. -
Western blot - Anti-WASP/Wiskott-Aldrich syndrome protein (phospho Y290) antibody (ab59278)All lanes : Anti-WASP/Wiskott-Aldrich syndrome protein (phospho Y290) antibody (ab59278) at 1/500 dilution
Lane 1 : Extracts from HepG2 cells with no immunising peptide
Lane 2 : Extracts from HepG2 cells with immunising peptide
Predicted band size: 53 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab59278 は 5 報の論文で使用されています。
- Kumari S et al. Cytoskeletal tension actively sustains the migratory T-cell synaptic contact. EMBO J 39:e102783 (2020). PubMed: 31894880
- Ganesan R et al. Oxidized LDL phagocytosis during foam cell formation in atherosclerotic plaques relies on a PLD2-CD36 functional interdependence. J Leukoc Biol 103:867-883 (2018). PubMed: 29656494
- Liu C et al. N-wasp is essential for the negative regulation of B cell receptor signaling. PLoS Biol 11:e1001704 (2013). Flow Cyt, ICC/IF ; Mouse, Human . PubMed: 24223520
- Malet-Engra G et al. CIP4 controls CCL19-driven cell steering and chemotaxis in chronic lymphocytic leukemia. Cancer Res 73:3412-24 (2013). WB ; Human . PubMed: 23644527
- Kumar S et al. Cdc42 regulates neutrophil migration via crosstalk between WASp, CD11b, and microtubules. Blood 120:3563-74 (2012). PubMed: 22932798