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AB109537

Anti-WAPL/FOE 抗体 [EPR3407(2)]

Anti-WAPL/FOE antibody [EPR3407(2)]

3

(1 Review)

|

(2 Publications)

Rabbit Recombinant Monoclonal WAPL/FOE antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications.

別名を表示する

FOE, KIAA0261, WAPAL, WAPL, Wings apart-like protein homolog, Friend of EBNA2 protein, WAPL cohesin release factor

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-WAPL/FOE antibody [EPR3407(2)] (AB109537)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-WAPL/FOE antibody [EPR3407(2)] (AB109537)

ab109537 at 1/100 dilution staining WAPL/FOE in HeLa (Human epithelial cell line from cervix adenocarcinoma) cells by Immunofluorescence.

Western blot - Anti-WAPL/FOE antibody [EPR3407(2)] (AB109537)
  • WB

AbReview32141****

Western blot - Anti-WAPL/FOE antibody [EPR3407(2)] (AB109537)

All lanes:

Western blot - Anti-WAPL/FOE antibody [EPR3407(2)] (ab109537) at 1/10000 dilution

Lane 1:

Human U2OS cells treated with siRNA control. at 90 µg

Lane 2:

Human U2OS cells treated with siRNA #1 against WAPL/FOE at 90 µg

Lane 3:

Human U2OS cells treated with siRNA #2 against WAPL/FOE at 90 µg

Secondary

All lanes:

Immunopure Pierce anti-rabbit HRP conjugated at 1/10000 dilution

Predicted band size: 133 kDa

Observed band size: 120 kDa,130 kDa,145 kDa,150 kDa

true

Western blot - Anti-WAPL/FOE antibody [EPR3407(2)] (AB109537)
  • WB

Unknown

Western blot - Anti-WAPL/FOE antibody [EPR3407(2)] (AB109537)

All lanes:

Western blot - Anti-WAPL/FOE antibody [EPR3407(2)] (ab109537) at 1/50000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

HRP-labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 133 kDa,74 kDa

Observed band size: 70 kDa

false

関連する標識済み抗体及び組成の異なる製品 (1)

  • Carrier free

    Anti-WAPL/FOE antibody [EPR3407(2)] - BSA and Azide free

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR3407(2)

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

WB, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50000 - 1/200000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>" } } }
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製品の詳細

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態
Liquid
精製度
Tissue culture supernatant
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Regulator of sister chromatid cohesion in mitosis which negatively regulates cohesin association with chromatin (PubMed : 26299517). Involved in both sister chromatid cohesion during interphase and sister-chromatid resolution during early stages of mitosis. Couples DNA replication to sister chromatid cohesion. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair.
See full target information WAPL
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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Genes & development 34:1619-1636 PubMed33122293

2020

Replication stress conferred by POT1 dysfunction promotes telomere relocalization to the nuclear pore.

Applications

Unspecified application

Species

Unspecified reactive species

Alexandra M Pinzaru,Mike Kareh,Noa Lamm,Eros Lazzerini-Denchi,Anthony J Cesare,Agnel Sfeir

The EMBO journal 35:2686-2698 PubMed27872142

2016

Cohesin acetylation and Wapl-Pds5 oppositely regulate translocation of cohesin along DNA.

Applications

Unspecified application

Species

Unspecified reactive species

Mai Kanke,Eri Tahara,Pim J Huis In't Veld,Tomoko Nishiyama
View all publications
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Abcam product promise

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