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Anti-VSV-G tag 抗体 [P5D4] (ab50549)

Reviews (1)Q&A (2)References (23)
Immunocytochemistry - Anti-VSV-G tag antibody [P5D4] (ab50549)
  • Western blot - Anti-VSV-G tag antibody [P5D4] (ab50549)

Key features and details

  • Mouse monoclonal [P5D4] to VSV-G tag
  • Suitable for: WB, IP, ICC
  • Reacts with: Species independent
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-VSV-G tag antibody [P5D4]
    VSV-G tag 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [P5D4] to VSV-G tag

  • 由来種

    Mouse

  • アプリケーション

    適用あり: WB, IP, ICCmore details

  • 種交差性

    交差種: Species independent

  • 免疫原

    Synthetic peptide corresponding to C terminal amino acids 497-511 of VSV-G tag conjugated to KLH

  • ポジティブ･コントロール

    • WB: Whole extract of HEK-293T cells over-expressing VSV-G-tagged Vinculin. ICC/IF: COS7 cells over-expressing VSV-G tagged vinculin.

  • 特記事項

    This product was changed from ascites to tissue culture supernatant on 25th October 2016. The following lot(s) is/are from ascites and is still in stock as of 25th October 2016- GR223940,  GR223940. Lot numbers other than GR223940 and GR223940 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

    In infected cells, ab50549 localizes the immature forms of VSV-G in the rough endoplasmic reticulum (RER) and in the cisternae of Golgi complex, as well as mature VSV-G at the cell surface and in the budding virus. The antibody does not stain the secreted form of VSV-G which lacks the membrane and the cytoplasmic domain.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.

  • バッファー

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • 精製度

    Proprietary Purification

  • 特記事項（精製）

    Purified from Tissue culture supernatant.

  • ポリ/モノ

    モノクローナル

  • クローン名

    P5D4

  • アイソタイプ

    IgG1

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab50549の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use a concentration of 0.5 - 1 µg/ml.
IP
Use at an assay dependent concentration.
ICC
Use at an assay dependent concentration.
特記事項
WB
Use a concentration of 0.5 - 1 µg/ml.
IP
Use at an assay dependent concentration.
ICC
Use at an assay dependent concentration.

ターゲット情報

  • 関連性

    Vesicular stomatitis virus (VSV), an enveloped RNA virus from the Rhabdoviridae family, is released from the plasma membrane of host cells by a process called budding. The glycoprotein (VSV-g) contains a domain in its extracellular membrane proximal stem that appears to be needed for efficient VSV budding.

  • 別名

    • Vesicular stomatitis virus glycoprotein tag antibody
    • VSV epitope tag antibody
    • VSV tag antibody
    • VSV-G epitope tag antibody
    • VSVG antibody
    • vsvg tag antibody
    see all

画像

  • Immunocytochemistry - Anti-VSV-G tag antibody [P5D4] (ab50549)
    Immunocytochemistry - Anti-VSV-G tag antibody [P5D4] (ab50549)

    COS7 cells over-expressing VSV-G tagged vinculin were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 5 μg/ml ab50549. The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.

  • Western blot - Anti-VSV-G tag antibody [P5D4] (ab50549)
    Western blot - Anti-VSV-G tag antibody [P5D4] (ab50549)
    Lane 1 : Anti-VSV-G tag antibody [P5D4] (ab50549) at 0.5 µg/ml
    Lane 2 : Negative control: without first antibody

    Lane 1 : Whole extract of HEK-293T cells over-expressing VSV-G-tagged Vinculin
    Lane 2 : Whole extract of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells over-expressing VSV-G-tagged Vinculin

    Secondary
    All lanes : Goat Anti-Mouse IgG-Peroxidase

参考文献 (23)

ab50549 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab50549 は 23 報の論文で使用されています。

  • Phillips EA  et al. Detection of viral RNAs at ambient temperature via reporter proteins produced through the target-splinted ligation of DNA probes. Nat Biomed Eng 7:1571-1582 (2023). PubMed: 37142844
  • Malikov V  et al. FEZ1 phosphorylation regulates HSPA8 localization and interferon-stimulated gene expression. Cell Rep 38:110396 (2022). PubMed: 35172151
  • Beznoussenko GV  et al. Comparison of the Cisterna Maturation-Progression Model with the Kiss-and-Run Model of Intra-Golgi Transport: Role of Cisternal Pores and Cargo Domains. Int J Mol Sci 23:N/A (2022). PubMed: 35408951
  • Wang Y  et al. Tim-4 reprograms cholesterol metabolism to suppress antiviral innate immunity by disturbing the Insig1-SCAP interaction in macrophages. Cell Rep 41:111738 (2022). PubMed: 36450259
  • Furey C  et al. TACC3 Regulates Microtubule Plus-End Dynamics and Cargo Transport in Interphase Cells. Cell Rep 30:269-283.e6 (2020). PubMed: 31914393
View all Publications for this product

レビューと Q&A

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レビューを送る 質問を送る

1-3 of 3 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-VSV-G tag antibody [P5D4]

 Good
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human embryonic kidney (HEK) 293T)
Permeabilization
Yes - 0.1%Tween in PBST
Specification
Human embryonic kidney (HEK) 293T
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative
Paraformaldehyde
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Verified customer

投稿 Dec 05 2023

Question

Product code: 50549

Inquiry: Dear sir or madam: I am looking for an antibody which has the ability to test VSV-G tag fused to N-terminal and C terminal.and I wonder if the antibody (ab50549) will fullfill my desire or it just has the ability to test VSV-G fused to carboxy terminal of the recombinant protein. thank you for your attention and patience.

Read More
Answer

Thank you for contacting us.


Unfortunately we have not tested this antibody with an N-terminal VSVG tagged protein. The epitope for this clone (P5D4) resides within the C-terminal five amino acids (NRLGK) of the 15-amino acid antigenic peptide (KRQIYTDIEMNRLGK) so as long as this sequence is still surface exposed on the recombinant protein this antibody should work, however you would need to test this for yourself.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question

Hello,



I am looking for an antibody for detecting whether my cells are expressing VSV-G (Western blot). I have found several ab on-line, but do you also have cell lysate as a positive control?



Best regards

Read More
Answer

Thank you for your enquiry and your interest in our products.

We have a few antibodies which detect VSV-G and are suitable for Western applications. Some of them are unconjugated so it is essential to apply a compatible secondary antibody for detection:

ab1874: https://www.abcam.com/vsv-g-tag-antibody-ab1874.html

ab27026: https://www.abcam.com/vsv-g-tag-antibody-ab27026.html

ab50549: https://www.abcam.com/vsv-g-tag-antibody-p5d4-ab50549.html

Some others are HRP-labelled so can be applied without any secondary antibodies:

ab3556: https://www.abcam.com/vsv-g-tag-antibody-hrp-ab3556.html

ab49610: https://www.abcam.com/vsv-g-tag-antibody-p5d4-hrp-ab49610.html

Currently, we do not sel lpositive control cell lysate for VSV-G. As long as the cells you are planning to use express the tag sequence: YTDIEMNRLGK; these antibodies should work fine.

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