Anti-VSIG4 抗体 [EPR26179-56] (ab300148)
![Immunocytochemistry/ Immunofluorescence - Anti-VSIG4 antibody [EPR26179-56] (ab300148)](https://www.abcam.co.jp/ps/products/300/ab300148/Images/AB300148-2-anti-vsig4-antibody-epr2617956-immunocytochemistry-human-pbmc.jpg "Immunocytochemistry/ Immunofluorescence - Anti-VSIG4 antibody [EPR26179-56] (ab300148)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR26179-56] to VSIG4
- Suitable for: ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-VSIG4 antibody [EPR26179-56]
VSIG4 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR26179-56] to VSIG4 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IFmore details
適用なし: IHC-P,IP or WB -
種交差性
交差種: Human
非交差種: Mouse, Rat -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- ICC/IF: Human PBMC (human peripheral blood mononuclear cell).
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR26179-56 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab300148の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
1/50.
|
| 特記事項 |
|---|
|
ICC/IF
1/50. |
ターゲット情報
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機能
Phagocytic receptor, strong negative regulator of T-cell proliferation and IL2 production. Potent inhibitor of the alternative complement pathway convertases. -
組織特異性
Abundantly expressed in several fetal tissues. In adult tissues, highest expression in lung and placenta. Expressed in resting macrophages. -
配列類似性
Contains 2 Ig-like (immunoglobulin-like) domains. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 11326 Human
- Omim: 300353 Human
- SwissProt: Q9Y279 Human
- Unigene: 8904 Human
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別名
- Complement receptor of the immunoglobulin superfamily antibody
- CRIg antibody
- Ig superfamily protein antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-VSIG4 antibody [EPR26179-56] (ab300148)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Human PBMC (human peripheral blood mononuclear cell) cells labelling VSIG4 with ab300148 at 1/50 (9.28 µg/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 µg/ml dilution) (Green). Confocal image showing cytoplasmic staining in subsets of human PBMC. Negative control: Jurkat (PMID: 16009265).
ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 µg/ml) dilution (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 µg/ml) dilution.
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![Anti-VSIG4 antibody [EPR26179-56] (AB300148)](https://www.abcam.co.jp/ps/products/300/ab300148/Images/AB300148-485681-Benefits-of-Recombinant-antibodies.jpg)
Anti-VSIG4 antibody [EPR26179-56] (AB300148)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab300148 は論文での使用が確認できていません。