Anti-VPS34 抗体 - N-terminal

• IP

Supplier Data

Immunoprecipitation - Anti-VPS34 antibody - N-terminal (AB227861)

VPS34 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract with 5 μg of ab227861. Western blot was performed from the immunoprecipitate using ab227861. Anti-Rabbit IgG was used as a secondary reagent.

Lane 1 : HeLa whole cell extract.

Lane 2 : Control IgG IP in HeLa whole cell extract.

Lane 3 : ab227861 IP in HeLa whole cell extract.

All lanes:

Immunoprecipitation - Anti-VPS34 antibody - N-terminal (ab227861)

Predicted band size: 102 kDa

false

• WB

Supplier Data

Western blot - Anti-VPS34 antibody - N-terminal (AB227861)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-VPS34 antibody - N-terminal (ab227861) at 1/1000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg

Lane 2:

A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg

Lane 3:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg

Lane 4:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg

Predicted band size: 102 kDa

false

• WB

Supplier Data

Western blot - Anti-VPS34 antibody - N-terminal (AB227861)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-VPS34 antibody - N-terminal (ab227861) at 1/1000 dilution

Lane 1:

Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg

Lane 2:

VPS34 shRNA transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg

Predicted band size: 102 kDa

false

• WB

Supplier Data

Western blot - Anti-VPS34 antibody - N-terminal (AB227861)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-VPS34 antibody - N-terminal (ab227861) at 1/1000 dilution

Lane 1:

U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate at 30 µg

Lane 2:

SK-N-SH (human neuroblastoma cell line) whole cell lysate at 30 µg

Lane 3:

IMR32 whole cell lysate at 30 µg

Lane 4:

SK-N-AS whole cell lysate at 30 µg

Predicted band size: 102 kDa

false

• WB

Supplier Data

Western blot - Anti-VPS34 antibody - N-terminal (AB227861)

5% SDS-PAGE gel.

All lanes:

Western blot - Anti-VPS34 antibody - N-terminal (ab227861) at 1/5000 dilution

Lane 1:

Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg

Lane 2:

VPS34 transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg

Predicted band size: 102 kDa

false