Anti-VPS24 抗体 [EPR12821] (ab175930)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12821] to VPS24
- Suitable for: WB, IHC-P, IP, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-VPS24 antibody [EPR12821]
VPS24 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR12821] to VPS24 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, IP, Flow Cyt (Intra)more details
適用なし: ICC/IF -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Recombinant fragment within Human VPS24. The exact sequence is proprietary.
Database link: Q9Y3E7 -
ポジティブ・コントロール
- 293T, HepG2, U87-MG, fetal brain lysates; human cervical carcinoma tissue, human prostate tissue, 293T cells
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR12821 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab175930の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 29 kDa.
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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IP |
1/10 - 1/100.
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Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
特記事項 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 29 kDa. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
IP
1/10 - 1/100. |
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Selectively binds to phosphatidylinositol 3,5-bisphosphate PtdIns(3,5)P2 and PtdIns(3,4)P2 in preference to other phosphoinositides tested. Involved in late stages of cytokinesis. Required for sorting/trafficking of EGF receptor. -
組織特異性
Widely expressed. Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. -
配列類似性
Belongs to the SNF7 family. -
ドメイン
The acidic C-terminus and the basic N-termminus are thought to render the protein in a closed, soluble and inactive conformation through an autoinhibitory intramolecular interaction. The open and active conformation, which enables membrane binding and oligomerization, is achieved by interaction with other cellular binding partners, probably including other ESCRT components. -
細胞内局在
Cytoplasm > cytosol. Membrane. Endosome. Late endosome membrane. Localizes to the midbody of dividing cells. - Information by UniProt
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参照データベース
- Entrez Gene: 51652 Human
- Entrez Gene: 66700 Mouse
- Entrez Gene: 282834 Rat
- Omim: 610052 Human
- SwissProt: Q9Y3E7 Human
- SwissProt: Q9CQ10 Mouse
- SwissProt: Q8CGS4 Rat
- Unigene: 591582 Human
see all -
別名
- VPS 24 antibody
- VPS24 antibody
- CGI 149 antibody
see all
画像
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All lanes : Anti-VPS24 antibody [EPR12821] (ab175930) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : U87-MG cell lysate
Lane 4 : Fetal brain tissue lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 29 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPS24 antibody [EPR12821] (ab175930)
Immunohistochemical analysis of paraffin-embedded human prostate tissue labeling VPS24 with ab175930 at 1/50 dilution
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPS24 antibody [EPR12821] (ab175930)
Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissue using labeling VPS24 with ab175930 at 1/50 dilution
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Western blot analysis on immunoprecipitation pellet from (1) HepG2 cells lysate or (2) 1X PBS (negative control) using ab175930 at 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
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Intracellular flow cytometric analysis of permeabilized 293T cells labeling VPS24 with ab175930 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab175930 は 2 報の論文で使用されています。
- Leidal AM et al. The LC3-conjugation machinery specifies the loading of RNA-binding proteins into extracellular vesicles. Nat Cell Biol 22:187-199 (2020). PubMed: 31932738
- Zhang XW et al. Disrupting the TRIB3-SQSTM1 interaction reduces liver fibrosis by restoring autophagy and suppressing exosome-mediated HSC activation. Autophagy 16:782-796 (2020). PubMed: 31286822