Anti-VPRBP 抗体 (ab245483)
Key features and details
- Rabbit polyclonal to VPRBP
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
-
製品名
Anti-VPRBP antibody
VPRBP 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to VPRBP -
由来種
Rabbit -
アプリケーション
適用あり: IP, WBmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide within Human VPRBP aa 25-75. The exact sequence is proprietary. (NP_055518.1).
Database link: Q9Y4B6 -
ポジティブ･コントロール
- WB: HeLa, HEK-293T and NIH/3T3 whole cell lysates. IP: HeLa whole cell lysate.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab245483 was affinity purified using an epitope specific to VPRBP immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab245483の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP |
Use at 2-5 µg/mg of lysate.
|
|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 169 kDa.
|
| 特記事項 |
|---|
|
IP
Use at 2-5 µg/mg of lysate. |
|
WB
1/2000 - 1/10000. Predicted molecular weight: 169 kDa. |
ターゲット情報
-
関連性
VprBP may act as a receptor for CUL4-DDB1 ubiquitin ligase complex. In case of HIV1 infection, this protein is recruited by HIV1 VPR protein which is believed to result in an arrest of the cell cyccle in the G2 phase. -
細胞内局在
Cytoplasmic and Nuclear -
参照データベース
- Entrez Gene: 9730 Human
- Entrez Gene: 321006 Mouse
- SwissProt: Q9Y4B6 Human
- SwissProt: Q80TR8 Mouse
-
別名
- DCAF1 antibody
- DDB1 and CUL4 associated factor 1 antibody
- HIV-1 Vpr-binding protein antibody
see all
画像
-
Western blot - Anti-VPRBP antibody (ab245483)All lanes : Anti-VPRBP antibody (ab245483) at 0.04 µg
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 169 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-VPRBP antibody (ab245483)VPRBP was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245483 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab245483 at 1 µg/ml.
Lane 1: ab245483 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.Detection: Chemiluminescence with exposure time of 10 seconds.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab245483 は論文での使用が確認できていません。