Anti-VPH1 抗体 [10D7A7B2] (ab113683)
![Western blot - Anti-VPH1 antibody [10D7A7B2] (ab113683)](https://www.abcam.co.jp/ps/products/113/ab113683/Images/ab113683-126377-anti-vph1-antibody-10d7a7b2-western-blot.jpg "Western blot - Anti-VPH1 antibody [10D7A7B2] (ab113683)")
Key features and details
- Mouse monoclonal [10D7A7B2] to VPH1
- Suitable for: WB, ICC/IF
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG2a
製品の概要
-
製品名
Anti-VPH1 antibody [10D7A7B2] -
製品の詳細
Mouse monoclonal [10D7A7B2] to VPH1 -
由来種
Mouse -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Saccharomyces cerevisiae -
免疫原
Full length native protein (purified). This information is considered to be commercially sensitive.
-
特記事項
This IgG2a kappa isotype control monoclonal antibody was raised in mouse against a yeast-specific protein, V-ATPase subunit (the product of VPH1 gene). It is a suitable isotype control for mammalian species.
Isotype controls are used to confirm that the primary antibody binding is specific and not a result of non-specific Fc receptor binding or other protein interactions. The isotype control antibody should match the primary antibody’s host species, isotype, and possible conjugation.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by affinity purification. -
ポリ/モノ
モノクローナル -
クローン名
10D7A7B2 -
アイソタイプ
IgG2a -
軽鎖の種類
kappa -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab113683の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
Use a concentration of 0.25 - 1 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 95 kDa).
|
| ICC/IF |
Use a concentration of 20 µg/ml.
Heat induced antigen retrieval recommended. |
| 特記事項 |
|---|
|
WB
Use a concentration of 0.25 - 1 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 95 kDa). |
|
ICC/IF
Use a concentration of 20 µg/ml. Heat induced antigen retrieval recommended. |
ターゲット情報
-
機能
Subunit of the integral membrane V0 complex of vacuolar ATPase essential for assembly and catalytic activity. Is present only in vacuolar V-ATPase complexes. Enzymes containing this subunit have a 4-fold higher ratio of proton transport to ATP hydrolysis than complexes containing the Golgi/endosomal isoform and undergo reversible dissociation of V1 and V0 in response to glucose depletion. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. -
配列類似性
Belongs to the V-ATPase 116 kDa subunit family. -
翻訳後修飾
Glycosylated. -
細胞内局在
Vacuole membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 854444 Saccharomyces cerevisiae
- SwissProt: P32563 Saccharomyces cerevisiae
-
別名
- V-ATPase 95 kDa subunit antibody
- V-ATPase a 1 subunit antibody
- V-type proton ATPase subunit a antibody
see all
画像
-
Western blot - Anti-VPH1 antibody [10D7A7B2] (ab113683)All lanes : Anti-VPH1 antibody [10D7A7B2] (ab113683)
Lane 1 : Yeast whole cell lysate
Lane 2 : yeast membrane preparation
Lysates/proteins at 15 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 95 kDa
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (8)
ab113683 は 8 報の論文で使用されています。
- Shimasawa M et al. The pH-sensing Rim101 pathway regulates cell size in budding yeast. J Biol Chem 299:102973 (2023). PubMed: 36738789
- Hose J et al. The genetic basis of aneuploidy tolerance in wild yeast. Elife 9:N/A (2020). PubMed: 31909711
- Azevedo C et al. Development of a yeast model to study the contribution of vacuolar polyphosphate metabolism to lysine polyphosphorylation. J Biol Chem 295:1439-1451 (2020). PubMed: 31844018
- Chen H et al. The Ccr4-Not complex regulates TORC1 signaling and mitochondrial metabolism by promoting vacuole V-ATPase activity. PLoS Genet 16:e1009046 (2020). PubMed: 33064727
- Karim MA et al. Rab-Effector-Kinase Interplay Modulates Intralumenal Fragment Formation during Vacuole Fusion. Dev Cell 47:80-97.e6 (2018). PubMed: 30269949
- Cavellini L et al. An ubiquitin-dependent balance between mitofusin turnover and fatty acids desaturation regulates mitochondrial fusion. Nat Commun 8:15832 (2017). WB ; Saccharomyces cerevisiae . PubMed: 28607491
- Dodgson SE et al. The pleiotropic deubiquitinase Ubp3 confers aneuploidy tolerance. Genes Dev 30:2259-2271 (2016). PubMed: 27807036
- Simpkins JA et al. Disruption of a cystine transporter downregulates expression of genes involved in sulfur regulation and cellular respiration. Biol Open 5:689-97 (2016). PubMed: 27142334