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Anti-Von Willebrand Factor 抗体 [F8/86] (ab778)

Reviews (3)Q&A (2)References (15)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Willebrand Factor antibody [F8/86] (ab778)

    Key features and details

    • Mouse monoclonal [F8/86] to Von Willebrand Factor
    • Suitable for: IHC-P, IHC-Fr
    • Reacts with: Human
    • Isotype: IgG1

    製品の概要

    • 製品名

      Anti-Von Willebrand Factor antibody [F8/86]
      Von Willebrand Factor 一次抗体 製品一覧

    • 製品の詳細

      Mouse monoclonal [F8/86] to Von Willebrand Factor

    • 由来種

      Mouse

    • アプリケーション

      適用あり: IHC-P, IHC-Frmore details

    • 種交差性

      交差種: Human
      交差が予測される動物種: Rabbit

    • 免疫原

      Full length native protein (purified) corresponding to Human Von Willebrand Factor. Von Willebrand Factor isolated from human plasma.

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab778の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    IHC-P (3)
    1/25 - 1/50. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
    IHC-Fr
    Use at an assay dependent concentration.

    ABC method. We suggest an incubation period of 60 minutes at room temperature.
    特記事項
    IHC-P
    1/25 - 1/50. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
    IHC-Fr
    Use at an assay dependent concentration.

    ABC method. We suggest an incubation period of 60 minutes at room temperature.

    ターゲット情報

    • 機能

      Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.

    • 組織特異性

      Plasma.

    • 関連疾患

      Defects in VWF are the cause of von Willebrand disease (VWD) [MIM:277480]. VWD defines a group of hemorrhagic disorders in which the von Willebrand factor is either quantitatively or qualitatively abnormal resulting in altered platelet function. Symptoms vary depending on severity and disease type but may include prolonged bleeding time, deficiency of factor VIII and impaired platelet adhesion. Type I von Willebrand disease is the most common form and is characterized by partial quantitative plasmatic deficiency of an otherwise structurally and functionally normal Willebrand factor; type II is associated with a qualitative deficiency and functional anomalies of the Willebrand factor; type III is the most severe form and is characterized by total or near-total absence of Willebrand factor in the plasma and cellular compartments, also leading to a profound deficiency of plasmatic factor VIII.

    • 配列類似性

      Contains 1 CTCK (C-terminal cystine knot-like) domain.
      Contains 4 TIL (trypsin inhibitory-like) domains.
      Contains 3 VWFA domains.
      Contains 3 VWFC domains.
      Contains 4 VWFD domains.

    • ドメイン

      The von Willebrand antigen 2 is required for multimerization of vWF and for its targeting to storage granules.

    • 翻訳後修飾

      All cysteine residues are involved in intrachain or interchain disulfide bonds.
      N- and O-glycosylated.

    • 細胞内局在

      Secreted. Secreted > extracellular space > extracellular matrix. Localized to storage granules.
    • Information by UniProt

    • 参照データベース

      • 別名

        • Coagulation factor VIII antibody
        • Coagulation factor VIII VWF antibody
        • F8VWF antibody
        • Factor VIII related antigen antibody
        • von Willebrand antigen 2 antibody
        • von Willebrand antigen II antibody
        • Von Willebrand disease antibody
        • Von Willebrand factor precursor antibody
        • VWD antibody
        • vWF antibody
        • VWF_HUMAN antibody
        see all

      画像

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Willebrand Factor antibody [F8/86] (ab778)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Willebrand Factor antibody [F8/86] (ab778)
        Formalin fixed paraffin embedded human tonsil tissue, staining von Willebrand factor with ab778 in immunohistochemical analysis

      参考文献 (15)

      ab778 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab778 は 15 報の論文で使用されています。

      • Yao HJ  et al. Three new experimental models of anterior urethral stricture in rabbits. Transl Androl Urol 11:761-772 (2022). PubMed: 35812200
      • Chen C  et al. A proteomics analysis of neointima formation on decellularized vascular grafts reveals regenerative alterations in protein signature running head: Proteomics analysis of neointima formation. Front Bioeng Biotechnol 10:894956 (2022). PubMed: 36406232
      • Liu T  et al. MiR-4729 regulates TIE1 mRNA m6A modification and angiogenesis in hemorrhoids by targeting METTL14. Ann Transl Med 9:232 (2021). PubMed: 33708859
      • Wan X  et al. Hypoxia-preconditioned adipose-derived stem cells combined with scaffold promote urethral reconstruction by upregulation of angiogenesis and glycolysis. Stem Cell Res Ther 11:535 (2020). PubMed: 33308306
      • Ko SF  et al. Inducible pluripotent stem cell-derived mesenchymal stem cell therapy effectively protected kidney from acute ischemia-reperfusion injury. Am J Transl Res 10:3053-3067 (2018). PubMed: 30416650
      View all Publications for this product

      レビューと Q&A

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      1-5 of 5 Abreviews or Q&A

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Von Willebrand Factor antibody [F8/86]

       Good
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: citrate buffer
      Sample
      Rabbit Tissue sections (trachea)
      Specification
      trachea
      Permeabilization
      Yes - triton X-100
      Fixative
      Paraformaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Ms. Hee-Bok Kim

      Verified customer

      投稿 Jun 05 2014

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Von Willebrand Factor antibody [F8/86]

       Poor
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Rabbit Tissue sections (Brain)
      Antigen retrieval step
      Enzymatic - Buffer/Enzyme Used: Proteinase K
      Permeabilization
      No
      Specification
      Brain
      Blocking step
      Dako Serum Free Protein Block as blocking agent for 5 minute(s) · Concentration: 0.25% · Temperature: 25°C
      Fixative
      Formaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      MR. Mark Butters

      Verified customer

      投稿 Aug 19 2022

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Von Willebrand Factor antibody [F8/86]

       Average
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Blocking step
      1% BSA + 5% NGS + 0.1% Sapo as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 20°C
      Antigen retrieval step
      None
      Sample
      Human Tissue sections (blood vessels)
      Specification
      blood vessels
      Permeabilization
      Yes - saponine
      Fixative
      Formaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      投稿 Mar 24 2014

      Question

      I've used your mouse monoclonal von willebrand Factor antibody (ab778)for IHC in my experiments. The data will be published. The edditors asked us to show the specificity of this antibody. Your data sheet showed 3 references. I checked them and found no one exactely used this antibody for IHC. Could you tell me where the image (a good staining)in the data sheet was cited from? Do you have the antigen for this antibody? Or could you tell me what company has this antigen? I will buy the antigen of this antobody to run the preabsorption experiment.

      Read More
      Answer

      Thank you for contacting us.

      The lab sent me the following information regarding your question:

      Based on the data Factor VIII reacts specifically with the endothelial cells of normal, reactive, and neoplastic blood and lymphatic vessels and shows finely granular cytoplasmic staining. It also reacts with endocardium, platelets, and megakaryocytes.

      Please let me know if this information is sufficient.

      Use our products? Submit an Abreview. Earn rewards!
      https://www.abcam.com/abreviews

      Read More

      Question

      I've used your mouse monoclonal von willebrand Factor antibody (ab778)for IHC in my experiments. The data will be published. The edditors asked us to show the specificity of this antibody. Your data sheet showed 3 references. I checked them and found no one exactely used this antibody for IHC. Could you tell me where the image (a good staining)in the data sheet was cited from? Do you have the antigen for this antibody? Or could you tell me what company has this antigen? I will buy the antigen of this antobody to run the preabsorption experiment.

      Read More
      Answer

      Thank you for contacting us.

      I have emailed the lab to check regarding the specificity testing, and I'll let you know what I find out.
      The IHC image on the datasheet is from the lab.

      The immunogen used to raise the antibody was Von Willebrand Factor isolated from human plasma. We carry vWF protein (ab88533 and ab88555) that can be used for blocking, although we have not tried that application with these 2 proteins.

      https://www.abcam.com/Von-Willebrand-Factor-protein-Human-ab88533.html
      https://www.abcam.com/Von-Willebrand-Factor-protein-Human-ab88555.html

      You can use our testing discount program to try either protein in a blocking experiment.

      For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:

      The testing discount program is for customers who like to use an antibody/protein/kit on an untested species/application. You would purchase the antibody/protein/kit at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody/protein at the full price (100%) of the antibody/protein you have tested, or a full price discount for the amount paid for the kit. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.
      This programapplies to ELISA kits and other kits we offer.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Use our products? Submit an Abreview. Earn rewards!
      https://www.abcam.com/abreviews

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com