AB154193

Anti-Von Willebrand Factor 抗体 [EPSISR15]

Anti-Von Willebrand Factor antibody [EPSISR15]

RabMAb
Recombinant
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(25 Publications)

Anti-Von Willebrand Factor antibody [EPSISR15] (ab154193) is a rabbit monoclonal antibody detecting Von Willebrand Factor in Western Blot, Flow Cytometry (Intra), ICC/IF. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency
- Over 10 publications

別名を表示する

F8VWF, VWF, von Willebrand factor, vWF

6 Images

Immunocytochemistry/ Immunofluorescence - Anti-Von Willebrand Factor antibody [EPSISR15] (AB154193)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Von Willebrand Factor antibody [EPSISR15] (AB154193)

Immunofluorescent staining of HepG2 cells labeling Von-Willebrand-Factor with unpurified ab154193 at 1/250 dilution.

Flow Cytometry (Intracellular) - Anti-Von Willebrand Factor antibody [EPSISR15] (AB154193)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Von Willebrand Factor antibody [EPSISR15] (AB154193)

Intracellular Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling Von Willebrand Factor (red) with purified ab154193 at a 1/130 dilution (10ug/mL). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti rabbit IgG (Alexa Fluor^® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Von Willebrand Factor antibody [EPSISR15] (AB154193)

Immunocytochemistry/ Immunofluorescence analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling Von Willebrand Factor with Purified ab154193 at 1 : 250 dilution. Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor ® 594) 1 : 200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor ® 488) was used as the secondary antibody at 1 : 1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Lab

Western blot - Anti-Von Willebrand Factor antibody [EPSISR15] (AB154193)

Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Von Willebrand Factor antibody [EPSISR15] (ab154193) at 1/1000 dilution

All lanes:

Human plasma lysates at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 221 kDa,309 kDa

Observed band size: 221 kDa,309 kDa

false

Lab

Western blot - Anti-Von Willebrand Factor antibody [EPSISR15] (AB154193)

Blocking and diluting buffer : 5% NFDM/TBST

The expression profile observed is consistent with the literature (PMID : 24769235) in which ab154193 was validated by knockdown assay in HepG2 cell line.

All lanes:

Western blot - Anti-Von Willebrand Factor antibody [EPSISR15] (ab154193) at 4 µg/mL

All lanes:

HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/20000 dilution

Predicted band size: 309 kDa

false

Exposure time: 30s

Unknown

Western blot - Anti-Von Willebrand Factor antibody [EPSISR15] (AB154193)

All lanes:

Western blot - Anti-Von Willebrand Factor antibody [EPSISR15] (ab154193) at 1/1000 dilution

Lane 1:

Human serum at 10 µg

Lane 2:

Human plasma at 10 µg

Predicted band size: 309 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPSISR15

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

ICC/IF, Flow Cyt (Intra), WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/200", "FlowCytIntra-species-notes": "" } } }

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製品の詳細

What is this antibody validated in?
Anti-Von Willebrand Factor antibody [EPSISR15] (ab154193) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

What is the molecular weight of Von Willebrand Factor?
Anti-Von Willebrand Factor [EPSISR15] (ab154193) specifically detects a band for Von Willebrand Factor (UniProt: P04275) at a molecular weight of 309kDa.

Trusted by the scientific community
Anti-Von Willebrand Factor [EPSISR15] (ab154193) was first used in a scientific publication in 2012 and has been cited over 10 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPSISR15] also available for your convenience: ab154193, Alexa Fluor® 488 - ab195028, Alexa Fluor® 647 - ab195029, HRP - ab195030, Carrier free - ab232473, APC - ab310828, PE - ab310904, Alexa Fluor® 594 - ab311713, Alexa Fluor® 568 - ab312989, Alexa Fluor® 555 - ab313194

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.

See full target information VWF

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文献 (25)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular medicine 56: PubMed40709382

2025

Dulaglutide markedly prevents peritoneal fibrosis in a rodent model of chronic kidney disease: Insights into the pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Chih-Chao Yang,Ya Yue,Yi-Ting Wang,John Y Chiang,Ben-Chung Cheng,Tsuen-Wei Hsu,Yi-Ling Chen,Yi-Chen Li,Hon-Kan Yip

JCI insight 10: PubMed40260916

2025

Reshaping the chromatin landscape in HUVECs from small-for-gestational-age newborns.

Applications

Unspecified application

Species

Unspecified reactive species

Lingling Yan,Zhimin Zhou,Shengcai Chen,Xin Feng,Junwen Mao,Fang Luo,Jianfang Zhu,Xiuying Chen,Yingying Hu,Yuan Wang,Bingbing Wu,Lizhong Du,Chunlin Wang,Liang Gong,Yanfen Zhu

Cell biology and toxicology 41:44 PubMed39937362

2025

Enhancing cardiac repair post-myocardial infarction: a study on GATM/Gel hydrogel therapeutics.

Applications

Unspecified application

Species

Unspecified reactive species

Te Li,Lijuan Ding,Qiang Wang,Jianing Ma,Shudong Wang

Cerebrovascular diseases extra 14:165-176 PubMed39419008

2024

siLOXL2 Inhibits Endothelial Inflammatory Response and EndMT Induced by ox-LDL.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Ma,Jia Ling,Rui Tong,Jiefen Guo,Zhongsheng Zhu

Biomolecules 14: PubMed38540749

2024

High Glucose Promotes and Aggravates the Senescence and Dysfunction of Vascular Endothelial Cells in Women with Hyperglycemia in Pregnancy.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Zheng,Mingqing Li,Huaping Li

Advanced biology 8:e2300513 PubMed38494421

2024

The α-1 Adrenergic Receptor Antagonist Doxazosin Attenuates Liver Fibrosis by Alleviating Sinusoidal Capillarization and Liver Angiogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Ai-Yuan Xiu,Qian Ding,Chang-Peng Zhu,Chun-Qing Zhang

International journal of molecular sciences 24: PubMed37686378

2023

Enhanced Angiogenesis in HUVECs Preconditioned with Media from Adipocytes Differentiated from Lipedema Adipose Stem Cells In Vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Al-Ghadban,Samantha G Walczak,Spencer U Isern,Elizabeth C Martin,Karen L Herbst,Bruce A Bunnell

Cell communication and signaling : CCS 21:176 PubMed37480055

2023

Neutrophil extracellular traps promote angiogenesis in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shifeng Yang,Boshi Sun,Jiacheng Li,Nana Li,Ange Zhang,Xinyu Zhang,Hao Yang,Xiaoming Zou

Journal of tissue engineering 13:20417314221143240 PubMed36600998

2022

Microvessels derived from hiPSCs are a novel source for angiogenesis and tissue regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Gao,Shixing Ma,Xiaotao Xing,Jian Yang,Xun Xu,Cheng Liang,Yejia Yu,Lei Liu,Li Liao,Weidong Tian

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 36:e22608 PubMed36250975

2022

Dexmedetomidine attenuates renal ischemia-reperfusion injury through activating PI3K/Akt-eNOS signaling via α adrenoreceptors in renal microvascular endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xisheng Shan,Jiaxin Zhang,Xiang Wei,Wenhui Tao,Ke Peng,Huayue Liu,Yiqing Wang,Hong Liu,Xiaowen Meng,Fuhai Ji

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Von Willebrand Factor 抗体 [EPSISR15]

Anti-Von Willebrand Factor antibody [EPSISR15]

Immunocytochemistry/ Immunofluorescence - Anti-Von Willebrand Factor antibody [EPSISR15] (AB154193)

Flow Cytometry (Intracellular) - Anti-Von Willebrand Factor antibody [EPSISR15] (AB154193)

Immunocytochemistry/ Immunofluorescence - Anti-Von Willebrand Factor antibody [EPSISR15] (AB154193)

Western blot - Anti-Von Willebrand Factor antibody [EPSISR15] (AB154193)

Western blot - Anti-Von Willebrand Factor antibody [EPSISR15] (AB154193)

Western blot - Anti-Von Willebrand Factor antibody [EPSISR15] (AB154193)

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Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (25)

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