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リコンビナントRabMAb

Anti-VMAT1 抗体 [EPR6476] (ab168347)

Submit a review Submit a question References (3)
Flow Cytometry (Intracellular) - Anti-VMAT1 antibody [EPR6476] (ab168347)
  • Western blot - Anti-VMAT1 antibody [EPR6476] (ab168347)
  • Anti-VMAT1 antibody [EPR6476] (ab168347)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6476] to VMAT1
  • Suitable for: Flow Cyt (Intra), WB
  • Reacts with: Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-VMAT1 antibody [EPR6476]
    VMAT1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR6476] to VMAT1

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), WBmore details
    適用なし: ICC/IF,IHC-P or IP

  • 種交差性

    交差種: Rat, Human
    交差が予測される動物種: Mouse

  • 免疫原

    Synthetic peptide within Human VMAT1. The exact sequence is proprietary.
    Database link: P54219

  • ポジティブ･コントロール

    • BxPC-3, PC-12 and A549 cell lysates, A549 cells

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab168347の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.  
WB
1/1000 - 1/5000. Predicted molecular weight: 56 kDa.
特記事項
Flow Cyt (Intra)
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.  
WB
1/1000 - 1/5000. Predicted molecular weight: 56 kDa.
追加情報
Is unsuitable for ICC/IF,IHC-P or IP.

ターゲット情報

画像

  • Flow Cytometry (Intracellular) - Anti-VMAT1 antibody [EPR6476] (ab168347)
    Flow Cytometry (Intracellular) - Anti-VMAT1 antibody [EPR6476] (ab168347)

    Intracellular flow cytometrical analysis of permeabilized A549 cells with ab168347 antibody at a dilution of 1/100 (red) or a rabbit IgG negative (green). 

     

     

  • Western blot - Anti-VMAT1 antibody [EPR6476] (ab168347)
    Western blot - Anti-VMAT1 antibody [EPR6476] (ab168347)
    All lanes : Anti-VMAT1 antibody [EPR6476] (ab168347) at 1/1000 dilution

    Lane 1 : BxPC-3 cell lysate
    Lane 2 : PC-12 cell lysate
    Lane 3 : A549 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 56 kDa

  • Anti-VMAT1 antibody [EPR6476] (ab168347)
    Anti-VMAT1 antibody [EPR6476] (ab168347)

参考文献 (3)

ab168347 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab168347 は 3 報の論文で使用されています。

  • Wu Q  et al. Dynamin 1 Restrains Vesicular Release to a Subquantal Mode In Mammalian Adrenal Chromaffin Cells. J Neurosci 39:199-211 (2019). PubMed: 30381405
  • Sato DX  et al. Human-specific mutations in VMAT1 confer functional changes and multi-directional evolution in the regulation of monoamine circuits. BMC Evol Biol 19:220 (2019). PubMed: 31791232
  • Zhang Q  et al. Differential Co-release of Two Neurotransmitters from a Vesicle Fusion Pore in Mammalian Adrenal Chromaffin Cells. Neuron 102:173-183.e4 (2019). PubMed: 30773347

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