Anti-VMA2 抗体 [13D11B2] (ab113684)
![Western blot - Anti-VMA2 antibody [13D11B2] (ab113684)](https://www.abcam.co.jp/ps/products/113/ab113684/Images/ab113684-126378-anti-vma2-antibody-13d11b2-western-blot.jpg "Western blot - Anti-VMA2 antibody [13D11B2] (ab113684)")
Key features and details
- Mouse monoclonal [13D11B2] to VMA2
- Suitable for: ICC/IF, WB
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG1
製品の概要
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製品名
Anti-VMA2 antibody [13D11B2] -
製品の詳細
Mouse monoclonal [13D11B2] to VMA2 -
由来種
Mouse -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Saccharomyces cerevisiae -
免疫原
Full length native protein (purified). This information is considered to be commercially sensitive.
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ポジティブ･コントロール
- Yeast membranes
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
精製度
Proprietary Purification -
特記事項（精製）
Near homogeneity as judged by SDS-PAGE (purity >95%). The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
ポリ/モノ
モノクローナル -
クローン名
13D11B2 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab113684の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use a concentration of 20 µg/ml.
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| WB |
Use a concentration of 0.25 - 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).
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| 特記事項 |
|---|
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ICC/IF
Use a concentration of 20 µg/ml. |
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WB
Use a concentration of 0.25 - 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa). |
ターゲット情報
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機能
Non-catalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. It is an electrogenic proton pump that generates a proton motive force of 180 mv, inside positive and acidic, in the vacuolar membrane vesicles. -
配列類似性
Belongs to the ATPase alpha/beta chains family. - Information by UniProt
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参照データベース
- Entrez Gene: 852424 Saccharomyces cerevisiae
- SwissProt: P16140 Saccharomyces cerevisiae
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別名
- V-ATPase 57 kDa subunit antibody
- V-ATPase subunit B antibody
- V-type proton ATPase subunit B antibody
see all
画像
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Western blot - Anti-VMA2 antibody [13D11B2] (ab113684)Anti-VMA2 antibody [13D11B2] (ab113684) + Yeast whole cell lysate at 15 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 58 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab113684 は 2 報の論文で使用されています。
- Calvo IA et al. The fission yeast FLCN/FNIP complex augments TORC1 repression or activation in response to amino acid (AA) availability. iScience 24:103338 (2021). PubMed: 34805795
- Belgareh-Touzé N et al. Ubiquitination of ERMES components by the E3 ligase Rsp5 is involved in mitophagy. Autophagy 13:114-132 (2017). WB ; Saccharomyces cerevisiae . PubMed: 27846375