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リコンビナントRabMAb

Anti-VLDL Receptor/VLDL-R 抗体 [EPR26178-72] (ab302917)

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Western blot - Anti-VLDL Receptor/VLDL-R antibody [EPR26178-72] (ab302917)
  • Western blot - Anti-VLDL Receptor/VLDL-R antibody [EPR26178-72] (AB302917)
  • Western blot - Anti-VLDL Receptor/VLDL-R antibody [EPR26178-72] (AB302917)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VLDL Receptor/VLDL-R antibody [EPR26178-72] (AB302917)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VLDL Receptor/VLDL-R antibody [EPR26178-72] (AB302917)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VLDL Receptor/VLDL-R antibody [EPR26178-72] (AB302917)
  • Anti-VLDL Receptor/VLDL-R antibody [EPR26178-72] (AB302917)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR26178-72] to VLDL Receptor/VLDL-R
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-VLDL Receptor/VLDL-R antibody [EPR26178-72]
    VLDL Receptor/VLDL-R 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR26178-72] to VLDL Receptor/VLDL-R

  • 由来種

    Rabbit

  • 特異性

    This antibody does not cross-react with human LDLR.

  • アプリケーション

    適用あり: WB, IHC-Pmore details
    適用なし: Flow Cyt or IP

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Human and mouse heart tissue lysate treated & untreated with Protein Deglycosylation Mix II; human, mouse and rat liver tissue lysate, human small intestine tissue lysate; 3T3-L1 treated & untreated with1µM Dexamethasone whole cell tissue lysate, THP-1, and Huh7 whole cell lysates; human VLDLR & His-tagged LDLR recombinant protein. IHC-P: Human cardiac muscle and pancreas tissue sections.

  • 特記事項

    ab302917 is unsuitable for mouse and rat IHC

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab302917の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000. Detects a band of approximately 100-150 kDa (predicted molecular weight: 96 kDa).
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
特記事項
WB
1/1000. Detects a band of approximately 100-150 kDa (predicted molecular weight: 96 kDa).
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
追加情報
Is unsuitable for Flow Cyt or IP.

ターゲット情報

  • 機能

    Binds VLDL and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. Binding to Reelin induces tyrosine phosphorylation of Dab1 and modulation of Tau phosphorylation.

  • 組織特異性

    Abundant in heart and skeletal muscle; also ovary and kidney; not in liver.

  • 関連疾患

    Defects in VLDLR are the cause of cerebellar ataxia mental retardation and dysequilibrium syndrome type 1 (CMARQ1) [MIM:224050]; also known as dysequilibrium syndrome (DES) or non-progressive cerebellar disorder with mental retardation. CMARQ1 is a congenital, non-progressive cerebellar ataxia associated with disturbed equilibrium, delayed ambulation, mental retardation and cerebellar hypoplasia. Additional features include short stature, strabismus, pes planus and, rarely, seizures.

  • 配列類似性

    Contains 3 EGF-like domains.
    Contains 8 LDL-receptor class A domains.
    Contains 6 LDL-receptor class B repeats.

  • 翻訳後修飾

    Ubiquitinated at Lys-839 by MYLIP leading to degradation.

  • 細胞内局在

    Membrane. Membrane > clathrin-coated pit.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • FLJ35024 antibody
    • Very low density lipoprotein receptor antibody
    • Very low-density lipoprotein receptor antibody
    • VLDL R antibody
    • VLDL receptor antibody
    • VLDL-R antibody
    • VLDLR antibody
    • VLDLR_HUMAN antibody
    • VLDLRCH antibody
    see all

画像

  • Western blot - Anti-VLDL Receptor/VLDL-R antibody [EPR26178-72] (ab302917)
    Western blot - Anti-VLDL Receptor/VLDL-R antibody [EPR26178-72] (ab302917)
    All lanes : Anti-VLDL Receptor/VLDL-R antibody [EPR26178-72] (ab302917) at 1/1000 dilution

    Lane 1 : Human VLDLR recombinant protein 10 ng
    Lane 2 : His-tagged human LDLR recombinant protein 10 ng

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

    Developed using the ECL technique.

    Predicted band size: 96 kDa
    Observed band size: 15-50 kDa why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    Blocking / Diluting buffer and concentration: 5% NFDM/TBST.

    This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

  • Western blot - Anti-VLDL Receptor/VLDL-R antibody [EPR26178-72] (AB302917)
    Western blot - Anti-VLDL Receptor/VLDL-R antibody [EPR26178-72] (AB302917)
    All lanes : Anti-VLDL Receptor/VLDL-R antibody [EPR26178-72] (ab302917) at 1/1000 dilution

    Lane 1 : Untreated 3T3-L1 (mouse embryonic fibroblast), whole cell lysate
    Lane 2 : 3T3-L1 treated with1µM Dexamethasone for 48h, whole cell lysate
    Lane 3 : THP-1 (human monocytic leukemia monocyte), whole cell lysate
    Lane 4 : Huh7 (Human hepatocellular carcinoma epithelial cell), whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 96 kDa
    Observed band size: 100-150 kDa why is the actual band size different from the predicted?



    Blocking / Diluting buffer and concentration: 5% NFDM/TBST

    The expression of VLDLR is upregulated in response to Dexamethasone treatment (PMID: 11960750).
    Low expression: Huh7 (PMID: 29289645)

    Exposure time:

    Lane 1 and 2: 180 seconds
    Lane 3 and 4: 48 seconds

  • Western blot - Anti-VLDL Receptor/VLDL-R antibody [EPR26178-72] (AB302917)
    Western blot - Anti-VLDL Receptor/VLDL-R antibody [EPR26178-72] (AB302917)
    All lanes : Anti-VLDL Receptor/VLDL-R antibody [EPR26178-72] (ab302917) at 1/1000 dilution

    Lane 1 : Untreated human heart tissue lysate at 30 µg
    Lane 2 : Human heart tissue lysate treated with Protein Deglycosylation Mix II at 30 µg
    Lane 3 : Human liver tissue lysate at 50 µg
    Lane 4 : Human small intestine tissue lysate at 50 µg
    Lane 5 : Untreated mouse heart tissue lysate at 30 µg
    Lane 6 : Mouse heart tissue lysate treated with Protein Deglycosylation Mix II at 30 µg
    Lane 7 : Mouse liver tissue lysate at 50 µg
    Lane 8 : Rat heart tissue lysate at 50 µg
    Lane 9 : Rat liver tissue lysate at 50 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/1000 dilution

    Predicted band size: 96 kDa
    Observed band size: 100-150 kDa why is the actual band size different from the predicted?


    Exposure time: 48 seconds


    Blocking / Diluting buffer and concentration: 5% NFDM/TBST

    VLDLR is a glycoprotein of approximately 150 kDa and detected as a 120 kDa band after treated with Protein Deglycosylation MIX II.

    Low expression: liver, small intestine (PMID: 7925422; PMID: 11960750; PMID: 29289645)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VLDL Receptor/VLDL-R antibody [EPR26178-72] (AB302917)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VLDL Receptor/VLDL-R antibody [EPR26178-72] (AB302917)

    Immunohistochemical analysis of paraffin-embedded human cardiac muscle tissue labeling VLDL Receptor/VLDL-R with ab302917 at 1/500 dilution (0.978 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection). Cytoplasmic staining on cardiac muscle. The section was incubated with ab302917 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Primary diluent was used instead of primary antibody, followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VLDL Receptor/VLDL-R antibody [EPR26178-72] (AB302917)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VLDL Receptor/VLDL-R antibody [EPR26178-72] (AB302917)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling VLDL Receptor/VLDL-R with ab302917 at 1/500 dilution (0.978 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection). Cytoplasmic staining on islet of human pancreas. The section was incubated with ab302917 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Primary diluent was used instead of primary antibody, followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VLDL Receptor/VLDL-R antibody [EPR26178-72] (AB302917)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VLDL Receptor/VLDL-R antibody [EPR26178-72] (AB302917)

    Immunohistochemical analysis of paraffin-embedded human liver tissue labeling VLDL Receptor/VLDL-R with ab302917 at 1/500 dilution (0.978 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection). Negative control: No staining on human liver was observed. The section was incubated with ab302917 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Primary diluent was used instead of primary antibody, followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.

  • Anti-VLDL Receptor/VLDL-R antibody [EPR26178-72] (AB302917)
    Anti-VLDL Receptor/VLDL-R antibody [EPR26178-72] (AB302917)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab302917 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab302917 は論文での使用が確認できていません。

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