Anti-Vitronectin/S-Protein 抗体 [EP781Y] (ab46808)
![Western blot - Anti-Vitronectin/S-Protein antibody [EP781Y] (ab46808)](https://www.abcam.co.jp/ps/products/46/ab46808/Images/ab46808-59073-anti-vitronectin-s-protein-antibody-ep781y-western-blot.jpg "Western blot - Anti-Vitronectin/S-Protein antibody [EP781Y] (ab46808)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP781Y] to Vitronectin/S-Protein
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-Vitronectin/S-Protein antibody [EP781Y]
Vitronectin/S-Protein 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP781Y] to Vitronectin/S-Protein -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human Vitronectin/S-Protein aa 100-200. The exact sequence is proprietary.
-
ポジティブ･コントロール
- WB: Human serum IHC-P: Human lung tissue. IP: Human serum
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Protein A purified -
特記事項（精製）
This antibody has not been purified. It is provided in tissue culture supernatant and storage buffer. -
ポリ/モノ
モノクローナル -
クローン名
EP781Y -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab46808の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 54,60,75 kDa (predicted molecular weight: 54 kDa).
|
|
| IP |
1/50.
|
|
| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 特記事項 |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 54,60,75 kDa (predicted molecular weight: 54 kDa). |
|
IP
1/50. |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
-
機能
Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.
Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity. -
組織特異性
Plasma. -
配列類似性
Contains 4 hemopexin repeats.
Contains 1 SMB (somatomedin-B) domain. -
ドメイン
The SMB domain mediates interaction with SERPINE1/PAI1. The heparin-binding domain mediates interaction with insulin. -
翻訳後修飾
Sulfated on 2 tyrosine residues.
N- and O-glycosylated.
Phosphorylation on Thr-69 and Thr-76 favors cell adhesion and spreading.
It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.
Phosphorylation sites are present in the extracellular medium. -
細胞内局在
Secreted, extracellular space. - Information by UniProt
-
参照データベース
- Entrez Gene: 7448 Human
- Omim: 193190 Human
- SwissProt: P04004 Human
- Unigene: 2257 Human
-
別名
- Complement S Protein antibody
- Epibolin antibody
- S Protein antibody
see all
画像
-
Western blot - Anti-Vitronectin/S-Protein antibody [EP781Y] (ab46808)Anti-Vitronectin/S-Protein antibody [EP781Y] (ab46808) at 1/20000 dilution + Human serum at 10 µg
Secondary
Goat anti-rabbit, HRP labelled. at 1/2000 dilution
Predicted band size: 54 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Additional bands at: 54 kDa (possible non-specific binding), 60 kDa (possible non-specific binding) -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitronectin/S-Protein antibody [EP781Y] (ab46808)](https://www.abcam.co.jp/ps/products/46/ab46808/Images/ab46808-59072-anti-vitronectin-s-protein-antibody-ep781y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitronectin/S-Protein antibody [EP781Y] (ab46808)This image shows human lung tissue treated with ab46808 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Immunoprecipitation - Anti-Vitronectin/S-Protein antibody [EP781Y] (ab46808)](https://www.abcam.co.jp/ps/products/46/ab46808/Images/ab46808-4-anti-vitronectin-s-protein-antibody-ep781y-immunoprecipitation-serum-human.jpg)
Immunoprecipitation - Anti-Vitronectin/S-Protein antibody [EP781Y] (ab46808)Purified ab46808 at 1/50 dilution (2µg) immunoprecipitating Vitronectin/S-Protein in Human serum.
Lane 1 (input): Human serum 10µg
Lane 2 (+): ab46808 + Human serum.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab46808 in Human serum.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/10,000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 65-75 kDa -
![Anti-Vitronectin/S-Protein antibody [EP781Y] (ab46808)](https://www.abcam.co.jp/ps/products/46/ab46808/Images/ab46808-3-benefits-of-recombinant-antibodies.png)
Anti-Vitronectin/S-Protein antibody [EP781Y] (ab46808)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (4)
ab46808 は 4 報の論文で使用されています。
- Ma J & Kan Z Circular RNA circ_0008274 enhances the malignant progression of papillary thyroid carcinoma via modulating solute carrier family 7 member 11 by sponging miR-154-3p. Endocr J 68:543-552 (2021). PubMed: 33473055
- Miura K & Yamashita K Mechanical Weakness of Thoracic Aorta Related to Aging or Dissection Predicted by Speed of Sound with Collagenase. Ultrasound Med Biol 45:3102-3115 (2019). PubMed: 31537389
- Shi K et al. Vitronectin significantly influences prognosis in osteosarcoma. Int J Clin Exp Pathol 8:11364-71 (2015). PubMed: 26617861
- Shen J et al. Increased expression levels of vitronectin in the maternal-fetal interface of placenta in early-onset severe preeclampsia. Mol Med Rep 7:53-8 (2013). PubMed: 23124223