Anti-Vitronectin/S-Protein 抗体 [A18] (ab23444)
Key features and details
- Mouse monoclonal [A18] to Vitronectin/S-Protein
- Suitable for: ELISA, IHC-P, Inhibition Assay, IHC-Fr
- Reacts with: Rabbit, Horse, Cow
- Isotype: IgG1
製品の概要
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製品名
Anti-Vitronectin/S-Protein antibody [A18]
Vitronectin/S-Protein 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [A18] to Vitronectin/S-Protein -
由来種
Mouse -
特異性
ab23444 is highly specific for Vitronectin/S-Protein. There is no evidence for cross reactivity with other connective tissue proteins (Fibronectin, Elastin, Collagen, Laminin).
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アプリケーション
適用あり: ELISA, IHC-P, Inhibition Assay, IHC-Frmore details
適用なし: WB -
種交差性
交差種: Rabbit, Horse, Cow
非交差種: Sheep, Human -
免疫原
Tissue, cells or virus corresponding to Cow Vitronectin/S-Protein. Lysed bovine corneal endothelial cells and extracellular matrix
Database link: Q3ZBS7 -
特記事項
ab23444 can be used to probe vitronectin conformation.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS, 2.9% Sodium chloride -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
A18 -
ミエローマ
Sp2/0 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab23444の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
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| IHC-P |
Use at an assay dependent concentration.
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| Inhibition Assay |
Use at an assay dependent concentration.
ab23444 inhibits integrin mediated cell adhesion to bovine vitronectin. |
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| IHC-Fr |
Use at an assay dependent concentration.
1/500 for PLP fixed samples. |
| 特記事項 |
|---|
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ELISA
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. |
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Inhibition Assay
Use at an assay dependent concentration. ab23444 inhibits integrin mediated cell adhesion to bovine vitronectin. |
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IHC-Fr
Use at an assay dependent concentration. 1/500 for PLP fixed samples. |
ターゲット情報
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機能
Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.
Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity. -
組織特異性
Plasma. -
配列類似性
Contains 4 hemopexin repeats.
Contains 1 SMB (somatomedin-B) domain. -
ドメイン
The SMB domain mediates interaction with SERPINE1/PAI1. The heparin-binding domain mediates interaction with insulin. -
翻訳後修飾
Sulfated on 2 tyrosine residues.
N- and O-glycosylated.
Phosphorylation on Thr-69 and Thr-76 favors cell adhesion and spreading.
It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.
Phosphorylation sites are present in the extracellular medium. -
細胞内局在
Secreted, extracellular space. - Information by UniProt
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参照データベース
- Entrez Gene: 507525 Cow
- SwissProt: P22458 Rabbit
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別名
- Complement S Protein antibody
- Epibolin antibody
- S Protein antibody
see all
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab23444 は 1 報の論文で使用されています。
- Cámara-Torres M et al. Tuning Cell Behavior on 3D Scaffolds Fabricated by Atmospheric Plasma-Assisted Additive Manufacturing. ACS Appl Mater Interfaces 13:3631-3644 (2021). PubMed: 33448783