AB46808

Anti-Vitronectin/S-Protein 抗体 [EP781Y]

Anti-Vitronectin/S-Protein antibody [EP781Y]

RabMAb
Recombinant
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(5 Publications)

Rabbit Recombinant Monoclonal Vitronectin/S-Protein antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 5 publications.

別名を表示する

Vitronectin, VN, S-protein, Serum-spreading factor, V75, VTN

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitronectin/S-Protein antibody [EP781Y] (AB46808)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitronectin/S-Protein antibody [EP781Y] (AB46808)

This image shows human lung tissue treated with ab46808 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Lab

Immunoprecipitation - Anti-Vitronectin/S-Protein antibody [EP781Y] (AB46808)

Purified ab46808 at 1/50 dilution (2μg) immunoprecipitating Vitronectin/S-Protein in Human serum.
Lane 1 (input) : Human serum 10μg
Lane 2 (+) : ab46808 + Human serum.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab46808 in Human serum.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/10,000 dilution) was used for Western blotting.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.
Observed band size : 65-75 kDa

All lanes:

Immunoprecipitation - Anti-Vitronectin/S-Protein antibody [EP781Y] (ab46808)

Predicted band size: 54 kDa

false

Unknown

Western blot - Anti-Vitronectin/S-Protein antibody [EP781Y] (AB46808)

All lanes:

Western blot - Anti-Vitronectin/S-Protein antibody [EP781Y] (ab46808) at 1/20000 dilution

All lanes:

Human serum at 10 µg

Secondary

All lanes:

Goat anti-rabbit, HRP labelled. at 1/2000 dilution

Predicted band size: 54 kDa

Observed band size: 54 kDa,60 kDa,75 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EP781Y

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

IP, IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" } } }

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製品の詳細

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

精製に関する特記事項

This antibody has not been purified. It is provided in tissue culture supernatant and storage buffer.

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

保管に関する情報

Stable for 12 months at -20°C

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.. Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity.

See full target information Vitronectin

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文献 (5)

Recent publications for all applications. Explore the full list and refine your search

The American journal of pathology 194:180-194 PubMed38029923

2023

Multilamellated Basement Membranes in the Capillary Network of Alveolar Capillary Dysplasia.

Applications

Unspecified application

Species

Unspecified reactive species

Jan C Kamp,Lavinia Neubert,Jonas C Schupp,Peter Braubach,Christoph Wrede,Florian Laenger,Tim Salditt,Jakob Reichmann,Tobias Welte,Arjang Ruhparwar,Fabio Ius,Nicolaus Schwerk,Anke K Bergmann,Sandra von Hardenberg,Matthias Griese,Christina Rapp,Karen M Olsson,Jan Fuge,Da-Hee Park,Marius M Hoeper,Danny D Jonigk,Lars Knudsen,Mark P Kuehnel

Endocrine journal 68:543-552 PubMed33473055

2021

Circular RNA circ_0008274 enhances the malignant progression of papillary thyroid carcinoma via modulating solute carrier family 7 member 11 by sponging miR-154-3p.

Applications

Unspecified application

Species

Unspecified reactive species

Jingjing Ma,Zhenghua Kan

Ultrasound in medicine & biology 45:3102-3115 PubMed31537389

2019

Mechanical Weakness of Thoracic Aorta Related to Aging or Dissection Predicted by Speed of Sound with Collagenase.

Applications

Unspecified application

Species

Unspecified reactive species

Katsutoshi Miura,Kanna Yamashita

International journal of clinical and experimental 8:11364-71 PubMed26617861

2015

Vitronectin significantly influences prognosis in osteosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Shi,Rui-Long Lan,Xuan Tao,Chao-Yang Wu,Hai-Feng Hong,Jian-Hua Lin

Molecular medicine reports 7:53-8 PubMed23124223

2012

Increased expression levels of vitronectin in the maternal‑fetal interface of placenta in early-onset severe preeclampsia.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Shen,Xiaoyan Ma,Fei Yi,Azhar Rasul,Manhua Cui,Tonghui Ma

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Vitronectin/S-Protein 抗体 [EP781Y]

Anti-Vitronectin/S-Protein antibody [EP781Y]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitronectin/S-Protein antibody [EP781Y] (AB46808)

Immunoprecipitation - Anti-Vitronectin/S-Protein antibody [EP781Y] (AB46808)

Western blot - Anti-Vitronectin/S-Protein antibody [EP781Y] (AB46808)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存温度

長期保存温度

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (5)

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組合せ製品

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise