Anti-Vitamin D3 抗体 [EPPTX-R6-42-12] - BSA and Azide free (ab236470)
![Competitive ELISA - Anti-Vitamin D3 antibody [EPPTX-R6-42-12] - BSA and Azide free (ab236470)](https://www.abcam.co.jp/ps/products/236/ab236470/Images/ab236470-312596-236470.jpg "Competitive ELISA - Anti-Vitamin D3 antibody [EPPTX-R6-42-12] - BSA and Azide free (ab236470)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPPTX-R6-42-12] to Vitamin D3 - BSA and Azide free
- Suitable for: Competitive ELISA
- Reacts with: Species independent
Related conjugates and formulations
製品の概要
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製品名
Anti-Vitamin D3 antibody [EPPTX-R6-42-12] - BSA and Azide free
Vitamin D3 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPPTX-R6-42-12] to Vitamin D3 - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: Competitive ELISAmore details -
種交差性
交差種: Species independent -
免疫原
Chemical/ Small Molecule corresponding to Vitamin D3. Vitamin-D-3-BSA.
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特記事項
ab236470 is the carrier-free version of ab229820.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPPTX-R6-42-12 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Conjugation kits
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab236470の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Competitive ELISA |
Use at an assay dependent concentration.
Use at 30-35 ng/mL. |
| 特記事項 |
|---|
|
Competitive ELISA
Use at an assay dependent concentration. Use at 30-35 ng/mL. |
ターゲット情報
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関連性
Vitamin D3, also known as cholecalciferol, is a form of vitamin D that is made by the human body. It is made in the skin when 7-dehydrocholesterol reacts with UVB ultraviolet light with wavelengths 290 to 315 nm. This can be found in sunlight when the sun is high enough above the horizon for UVB to penetrate the atmosphere and is responsible for the production of cholecalciferol. Up to 20,000 IU can be made in the skin only after one minimal erythemal dose of exposure, or until the skin just begins to turn pink. -
細胞内局在
Secreted -
別名
- cholecalciferol antibody
画像
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Competitive ELISA - Anti-Vitamin D3 antibody [EPPTX-R6-42-12] - BSA and Azide free (ab236470)
Competitive ELISA - Anti-Vitamin D3 antibody [EPPTX-R6-42-12] (ab229820)
Goat anti-rabbit IgG was coated onto 96-wells. Serial dilutions (0, 2.5, 5, 10, 25, 50, 100 ng/mL) of 25-OH-Vitamin-D (25 μL) were incubated with 20-25 ng/mL of ab229820 (50 μL) for 30 minutes by shaking.
This was followed by adding HRP-conjugated 25-OH-Vitamin-D (50 μL) into each well and incubating for another 30 minutes. After washing, 125 μL of Tetramethylbenzidine (TMB) substrate was added and incubated for 30 minutes without shaking for color development. OD was read at 450nm within 5 minutes.
This data was kindly provided by Pantex Division of Bio-Analysis, Inc.
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![Competitive ELISA - Anti-Vitamin D3 antibody [EPPTX-R6-42-12] - BSA and Azide free (ab236470)](https://www.abcam.co.jp/ps/products/236/ab236470/Images/ab236470-312597-2364702.jpg)
Competitive ELISA - Anti-Vitamin D3 antibody [EPPTX-R6-42-12] - BSA and Azide free (ab236470)Competitive ELISA - Anti-Vitamin D3 antibody [EPPTX-R6-42-12] (ab229820) used at 6.3 ng/ml.
This data was kindly provided by Pantex Division of Bio-Analysis, Inc.
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![Anti-Vitamin D3 antibody [EPPTX-R6-42-12] - BSA and Azide free (ab236470)](https://www.abcam.co.jp/ps/products/236/ab236470/Images/ab236470-3-benefits-of-recombinant-antibodies.png)
Anti-Vitamin D3 antibody [EPPTX-R6-42-12] - BSA and Azide free (ab236470)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab236470 は論文での使用が確認できていません。
