Anti-Vitamin D Receptor 抗体 (ab3508)

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Since this is a whole antiserum antibody, the concentration has not been determined. Typically the total protein concentration for these antibodies can range from 1 to 10 mg/ml (you could split the difference and estimate 5 mg/ml).

You may want to remove the additional protein blocking step after the primary antibody incubation. Plus he antigen retrieval step may be a bit too long. Typically 10 mins boiling in a microwave followed by 20 min cooling works well. Also, since this is a nuclear antigen, you should permeabilize with Triton X-100 if you're not already. What species are your samples from and what is the tissue type (i.e. rat spleen)?

I'm listing some references below which have cited the use of this antibody in IHC:

Arch Biochem Biophys. 2010 Feb 15;494(2):166-77.
"Identification of a highly specific and versatile vitamin D receptor antibody."
Author(s): Wang Y, Becklund BR, DeLuca HF
Number of Citations: 1

Biogerontology. 2008 Apr;9(2):109-18.
"Vitamin D receptor levels and binding are reduced in aged rat intestinal subcellular fractions."
Author(s): González Pardo V, Boland R, de Boland AR
Number of Citations: 1

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Ab3508 is an unpurified whole serum antibody, and as there are other proteins in the serum the concentration of specific IgG can not be measured. This antibody has been used in ChIP in 4 known publications, though it's not tested in ChIP in our own lab. I've looked through the references (attached) and in "Ab3508 ChIP 1" and the protocol references of "Ab3508 ChIP 2, the authors used 5 uL of ab3508 per chromatin pellet.

I am sorry that we don't have more information, but I hope this will be useful. If you have any further questions or if there is anything else that we can do for you, please let me know and I'll be happy to help.

Thank you for your enquiry. The resources I have available indicate that 1 ul of ab3508 should be a sufficient amount for immunoprecipitation assays. I would recommend using a minimum of 100 ug of sample. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.