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リコンビナントRabMAb

Anti-Vitamin D Binding protein 抗体 [EP2923Y] (ab81307)

Reviews (1) Submit a question References (5)
Western blot - Anti-Vitamin D Binding protein antibody [EP2923Y] (ab81307)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitamin D Binding protein antibody [EP2923Y] (ab81307)
  • Anti-Vitamin D Binding protein antibody [EP2923Y] (ab81307)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP2923Y] to Vitamin D Binding protein
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Vitamin D Binding protein antibody [EP2923Y]
    Vitamin D Binding protein 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EP2923Y] to Vitamin D Binding protein

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-P, ICC/IFmore details
    適用なし: Flow Cyt or IP

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide within Human Vitamin D Binding protein aa 250-350. The exact sequence is proprietary.

  • ポジティブ･コントロール

    • Human liver tissue. Human plasma.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab81307の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (1)
1/5000 - 1/20000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.
特記事項
WB
1/5000 - 1/20000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.
追加情報
Is unsuitable for Flow Cyt or IP.

ターゲット情報

  • 機能

    Multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid, and urine and on the surface of many cell types. In plasma, it carries the vitamin D sterols and prevents polymerization of actin by binding its monomers. DBP associates with membrane-bound immunoglobulin on the surface of B-lymphocytes and with IgG Fc receptor on the membranes of T-lymphocytes.

  • 配列類似性

    Belongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.

  • 細胞内局在

    Secreted.
  • Information by UniProt

  • 参照データベース

    • 別名

      • DBP antibody
      • DBP/GC antibody
      • GC antibody
      • Gc globulin antibody
      • Gc-globulin antibody
      • GRD3 antibody
      • Group specific component antibody
      • Group specific component vitamin D binding protein antibody
      • Group-specific component antibody
      • hDBP antibody
      • VDB antibody
      • VDBG antibody
      • VDBP antibody
      • Vitamin D binding alpha globulin antibody
      • Vitamin D-binding protein antibody
      • VTDB_HUMAN antibody
      see all

    画像

    • Western blot - Anti-Vitamin D Binding protein antibody [EP2923Y] (ab81307)
      Western blot - Anti-Vitamin D Binding protein antibody [EP2923Y] (ab81307)
      Anti-Vitamin D Binding protein antibody [EP2923Y] (ab81307) at 1/20000 dilution + Human plasma lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/2000 dilution

      Predicted band size: 53 kDa
      Observed band size: 52 kDa why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      Blocking and Diluting buffer 5% NFDM /TBST 

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitamin D Binding protein antibody [EP2923Y] (ab81307)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitamin D Binding protein antibody [EP2923Y] (ab81307)

      Immunohistochemical analysis of paraffin-embedded human liver using ab81307 at a 1/100 dilution. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

    • Anti-Vitamin D Binding protein antibody [EP2923Y] (ab81307)
      Anti-Vitamin D Binding protein antibody [EP2923Y] (ab81307)

    参考文献 (5)

    ab81307 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab81307 は 5 報の論文で使用されています。

    • Xiao K  et al. Potential roles of vitamin D binding protein in attenuating liver injury in sepsis. Mil Med Res 9:4 (2022). PubMed: 35057868
    • Keeratichamroen S  et al. Identification of potential cervical cancer serum biomarkers in Thai patients. Oncol Lett 19:3815-3826 (2020). PubMed: 32391095
    • Baralla A  et al. Plasma Proteomic Signatures in Early Chronic Obstructive Pulmonary Disease. Proteomics Clin Appl 12:e1700088 (2018). PubMed: 29412517
    • Petrov B  et al. Bipolar disorder in youth is associated with increased levels of vitamin D-binding protein. Transl Psychiatry 8:61 (2018). PubMed: 29531242
    • Li J  et al. Single-cell transcriptomes reveal characteristic features of human pancreatic islet cell types. EMBO Rep 17:178-87 (2016). PubMed: 26691212

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Western blot abreview for Anti-Vitamin D Binding protein antibody [EP2923Y]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Brain lysate, Liver lysate and human serum)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris)
    Loading amount
    30 µg
    Specification
    Brain lysate, Liver lysate and human serum
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Ruma Raha-Chowdhury

    Verified customer

    投稿 Jan 28 2016

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