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Anti-Vitamin D Binding protein 抗体 [2G2] (ab23480)

Submit a review Q&A (2)References (3)

Key features and details

  • Mouse monoclonal [2G2] to Vitamin D Binding protein
  • Suitable for: ELISA, WB
  • Reacts with: Human
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-Vitamin D Binding protein antibody [2G2]
    Vitamin D Binding protein 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [2G2] to Vitamin D Binding protein

  • 由来種

    Mouse

  • 特異性

    ab23480 is specific for Gc-globulin (vitamin D binding protein).

  • アプリケーション

    適用あり: ELISA, WBmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Full length native Gc-globulin isolated from human plasma

  • ポジティブ･コントロール

    • Human plasma can be used as a positive control. The concentration of Gc-globulin in human plasma is approximately 400 µg/ml.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab23480の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ELISA
1/20000.
WB
Use at an assay dependent concentration. Detects a band of approximately 50 kDa.

(Note: In Western blotting after SDS-PAGE, HYB 249-01 reacts with Gc-globulin in both reduced as well as unreduced forms.)
特記事項
ELISA
1/20000.
WB
Use at an assay dependent concentration. Detects a band of approximately 50 kDa.

(Note: In Western blotting after SDS-PAGE, HYB 249-01 reacts with Gc-globulin in both reduced as well as unreduced forms.)

ターゲット情報

  • 機能

    Multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid, and urine and on the surface of many cell types. In plasma, it carries the vitamin D sterols and prevents polymerization of actin by binding its monomers. DBP associates with membrane-bound immunoglobulin on the surface of B-lymphocytes and with IgG Fc receptor on the membranes of T-lymphocytes.

  • 配列類似性

    Belongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.

  • 細胞内局在

    Secreted.
  • Information by UniProt

  • 参照データベース

    • 別名

      • DBP antibody
      • DBP/GC antibody
      • GC antibody
      • Gc globulin antibody
      • Gc-globulin antibody
      • GRD3 antibody
      • Group specific component antibody
      • Group specific component vitamin D binding protein antibody
      • Group-specific component antibody
      • hDBP antibody
      • VDB antibody
      • VDBG antibody
      • VDBP antibody
      • Vitamin D binding alpha globulin antibody
      • Vitamin D-binding protein antibody
      • VTDB_HUMAN antibody
      see all

    参考文献 (3)

    ab23480 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab23480 は 3 報の論文で使用されています。

    • Luebbering N  et al. Endothelial injury, F-actin and vitamin-D binding protein after hematopoietic stem cell transplant and association with clinical outcomes. Haematologica 106:1321-1329 (2021). PubMed: 32241849
    • Pottiez G  et al. Plasma proteomic profiling in HIV-1 infected methamphetamine abusers. PLoS One 7:e31031 (2012). WB ; Human . PubMed: 22359561
    • Bai S  et al. Proteome analysis of biomarkers in the cerebrospinal fluid of neuromyelitis optica patients. Mol Vis 15:1638-48 (2009). WB ; Human . PubMed: 19710940

    レビューと Q&A

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    レビューを送る 質問を送る

    1-2 of 2 Abreviews or Q&A

    Question

    Dear technical team,

    I purchased ab23480, ab121060, ab90920 and would like to do sandwich ELISA.
    I would like to know epitope sequences of ab23480 and ab121060. In addition, May I ask if ab23480 can be used as capture antibody and ab121063 (instead of ab121060) as detection antibody?

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    Answer

    Thank you for contacting us.

    Full length vitamin D binding protein is used as immunogen for ab121060, ab121063 and ab23480 but we are sorry to tell that we do not have a specific epitope mapping, down to the amino acid sequence, available. These antibodies have been tested in inhibition ELISA experiment, none of the antibodies inhibit each other. Hence, we believe that they do all react with different epitopes. However, as we have not tested ab23480 in sandwich ELISA with ab121063, we cannot tell whether ab23480 can be a suitable sandwich ELISA pair with ab121063, of which you have to test in your own experiment.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any further advice or information

    Read More

    Question

    We would like to purchase the positive control of this antibody. Could you please check if you have this product? https://www.abcam.com/index.html?pageconfig=datasheet&intAbID=23480 Thank you!

    Read More
    Answer

    Thank you for your enquiry. Further to correspondence with the source of this antibody I can tell you that normal human plasma should be suitable as a positive control. The concentration of Gc-globulin in human plasma is approximately 400 µg/ml (Masuda S, Okano T, Osawa K, Shinjo M, Suematsu T, Kobayashi T (1989) Concentrations of vitamin D-binding protein and vitamin D metabolites in plasma of patients with liver cirrosis. J Nutr Sci Vitaminol 35:225-34.). I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

    Read More

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