Anti-Vitamin D Binding protein 抗体 [19E8] (ab23485)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitamin D Binding protein antibody [19E8] (ab23485)](https://www.abcam.co.jp/ps/products/23/ab23485/Images/Vitamin-D-Binding-protein-Primary-antibodies-ab23485-1.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitamin D Binding protein antibody [19E8] (ab23485)")
Key features and details
- Mouse monoclonal [19E8] to Vitamin D Binding protein
- Suitable for: ELISA, IHC-P
- Reacts with: Human
- Isotype: IgG2
製品の概要
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製品名
Anti-Vitamin D Binding protein antibody [19E8]
Vitamin D Binding protein 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [19E8] to Vitamin D Binding protein -
由来種
Mouse -
アプリケーション
適用あり: ELISA, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Vitamin D Binding Protein isolated from human plasma.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Store In the Dark. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS, 2.9% Sodium chloride -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
19E8 -
ミエローマ
x63-Ag8.653 -
アイソタイプ
IgG2 -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab23485の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
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| IHC-P |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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ELISA
Use at an assay dependent concentration. |
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IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid, and urine and on the surface of many cell types. In plasma, it carries the vitamin D sterols and prevents polymerization of actin by binding its monomers. DBP associates with membrane-bound immunoglobulin on the surface of B-lymphocytes and with IgG Fc receptor on the membranes of T-lymphocytes. -
配列類似性
Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 2638 Human
- Omim: 139200 Human
- SwissProt: P02774 Human
- Unigene: 418497 Human
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別名
- DBP antibody
- DBP/GC antibody
- GC antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitamin D Binding protein antibody [19E8] (ab23485)IHC image of ab23485 staining in human liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab23485, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab23485 は論文での使用が確認できていません。