AB227850

Anti-VIP 抗体 - C-terminal

Name: Anti-VIP 抗体 - C-terminal (ab227850) | Abcam
Brand: Abcam Limited
SKU: AB227850
Price: 74000 JPY
Availability: InStock

Anti-VIP antibody - C-terminal

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(3 Publications)

Rabbit Polyclonal VIP antibody. C-terminal. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human VIP.

別名を表示する

VIP peptides, VIP

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VIP antibody - C-terminal (AB227850)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VIP antibody - C-terminal (AB227850)

Immunohistochemical analysis of paraffin-embedded mouse brain tissue labelling VIP protein at suprachiasmatic nucleus with ab227850 at a 1/100 dilution.

Antigen retrieval : Tris-EDTA buffer, pH 9.0, 15 min

Supplier Data

Western blot - Anti-VIP antibody - C-terminal (AB227850)

Samples were separated by 15% SDS-PAGE. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-VIP antibody - C-terminal (ab227850) at 1/3000 dilution

Lane 1:

U87-MG whole cell extracts at 30 µg

Lane 2:

SK-N-SH whole cell extracts at 30 µg

Lane 3:

IMR32 whole cell extracts at 30 µg

Lane 4:

SK-N-AS whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Human

アプリケーション

WB, IHC-P

applications

免疫原

Synthetic Peptide within Human VIP. The exact immunogen used to generate this antibody is proprietary information.

P01282

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Sheep": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Vasoactive Intestinal Peptide (VIP) also known as VIP or Alexa VIP is a 28-amino acid neuropeptide with a molecular mass of approximately 3326 Da. VIP is broadly expressed in both the central and peripheral nervous systems. It plays a mechanical role as a neurotransmitter and neuromodulator impacting various physiological functions such as vasodilation regulation of prolactin secretion and modulation of intestinal motility. By engaging in these processes VIP affects multiple bodily systems and acts at many sites within the human body.

Biological function summary

This protein influences immune response smooth muscle activity and circadian rhythm. VIP operates independently and as part of a complex in the body interacting with specific receptor types on the cellular surface like VPAC1 and VPAC2 receptors which are part of the G-protein coupled receptors family. VIP’s role as a regulator of immune response includes modulation of cytokine production and influencing lymphocyte activity implicating it as a significant player in homeostasis.

Pathways

A well-known function of VIP involves its participation in the cAMP signaling pathway which contributes to various cellular responses like neurotransmitter release and neuropeptide-mediated pathways influencing endocrine systems. VIP's activity in these pathways connects it to other proteins such as adenylate cyclase which it influences to increase cAMP levels further affecting processes such as synaptic transmission and the regulation of circadian rhythms. Through these pathways VIP affects physiological processes across the nervous and endocrine systems coordinating different aspects of bodily function.

VIP has notable associations with conditions such as inflammatory bowel disease (IBD) and asthma. The modulation of inflammatory processes by VIP impacts these diseases by influencing immune responses which can either exacerbate or alleviate symptoms. In IBD VIP interacts with elements of the immune system reducing inflammation and helping maintain mucosal integrity. In asthma VIP can alter airway inflammation and smooth muscle contraction through its pathways connected to the immune system and smooth muscle tissues interacting with proteins like cytokines that play roles in these conditions.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

VIP causes vasodilation, lowers arterial blood pressure, stimulates myocardial contractility, increases glycogenolysis and relaxes the smooth muscle of trachea, stomach and gall bladder.. PHM and PHV also cause vasodilation. PHM-27 is a potent agonist of the calcitonin receptor CALCR, with similar efficacy as calcitonin.

See full target information VIP

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Annals of gastroenterology 37:22-30 PubMed38223240

2024

Chronic constipation in Parkinson's disease: clinical features and molecular insights on the intestinal epithelial barrier.

Applications

Unspecified application

Species

Unspecified reactive species

Alexandros Ioannou,Anna Costanzini,Fiorella Giancola,Luis Cabanillas,Lisa Lungaro,Francesca Manza,Matteo Guarino,Rosario Arena,Giacomo Caio,Francesco Torresan,Andreas Polydorou,Antonios Vezakis,George Karamanolis,Catia Sternini,Roberto De Giorgio

Cellular and molecular gastroenterology and hepatology 17:383-398 PubMed38061549

2023

Vasoactive Intestinal Polypeptide Plays a Key Role in the Microbial-Neuroimmune Control of Intestinal Motility.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaopeng Bai,Giada De Palma,Elisa Boschetti,Yuichiro Nishiharo,Jun Lu,Chiko Shimbori,Anna Costanzini,Zarwa Saqib,Narjis Kraimi,Sacha Sidani,Siegfried Hapfelmeier,Andrew J Macpherson,Elena F Verdu,Roberto De Giorgio,Stephen M Collins,Premysl Bercik

World journal of surgical oncology 21:95 PubMed36915121

2023

Apigenin reduces the suppressive effect of exosomes derived from irritable bowel syndrome patients on the autophagy of human colon epithelial cells by promoting ATG14.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Fu,Saiyue Liu,Mingjin Zhu,Jiajie Zhu,Mingxian Chen

View all publications

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