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RabMAb

Anti-Vimentin (phospho S82) 抗体 [EP1071Y] (ab52943)

Submit a review Submit a question References (4)
Dot Blot - Anti-Vimentin (phospho S83) antibody [EP1071Y] (ab52943)
  • Western blot - Anti-Vimentin (phospho S83) antibody [EP1071Y] (ab52943)

Key features and details

  • Rabbit monoclonal [EP1071Y] to Vimentin (phospho S82)
  • Suitable for: Dot blot, WB
  • Reacts with: Human
  • Isotype: IgG

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Vimentin (phospho S82) antibody [EP1071Y]
    Vimentin 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EP1071Y] to Vimentin (phospho S82)

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Dot blot, WBmore details
    適用なし: Flow Cyt,IHC-P or IP

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HeLa cell lysate (AP untreated) + Calyculin A.

  • 特記事項

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab52943の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Dot blot
1/1000.
WB
1/500000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
特記事項
Dot blot
1/1000.
WB
1/500000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
追加情報
Is unsuitable for Flow Cyt,IHC-P or IP.

ターゲット情報

  • 機能

    Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
    Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.

  • 組織特異性

    Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.

  • 関連疾患

    Cataract 30

  • 配列類似性

    Belongs to the intermediate filament family.

  • ドメイン

    The central alpha-helical coiled-coil rod region mediates elementary homodimerization.
    The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.

  • 翻訳後修飾

    Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
    O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
    S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.

  • 細胞内局在

    Cytoplasm.
  • Information by UniProt

  • 参照データベース

    see all

  • 製品の状態

    Vimentin is found in connective tissue and in the cytoskeleton.

  • 別名

    • CTRCT30 antibody
    • Epididymis luminal protein 113 antibody
    • FLJ36605 antibody
    • HEL113 antibody
    • VIM antibody
    • VIME_HUMAN antibody
    • Vimentin antibody
    see all

画像

  • Dot Blot - Anti-Vimentin (phospho S83) antibody [EP1071Y] (ab52943)
    Dot Blot - Anti-Vimentin (phospho S83) antibody [EP1071Y] (ab52943)

    Primary antibody dilution: 1/1000

    Secondary antibody: goat anti-rabbit IgG, (H+L), peroxidase conjugated

    Secondary antibody dilution: 1/2500

    Blocking and dilution buffer: 5% NFDM/TBST

    Lane 1 sample: Vimentin (pS83) phospho peptide

    Lane 2 sample: Vimentin non-phospho peptide

    Exposure time: 3 minutes

  • Western blot - Anti-Vimentin (phospho S83) antibody [EP1071Y] (ab52943)
    Western blot - Anti-Vimentin (phospho S83) antibody [EP1071Y] (ab52943)
    All lanes : Anti-Vimentin (phospho S82) antibody [EP1071Y] (ab52943) at 1/500000 dilution

    Lane 1 : HeLa cell lysate AP treated
    Lane 2 : HeLa cell lysate (AP untreated) + Calyculin A

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 54 kDa
    Observed band size: 54 kDa

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (4)

ab52943 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab52943 は 4 報の論文で使用されています。

  • Liu J  et al. Bnip3 interacts with vimentin, an intermediate filament protein, and regulates autophagy of hepatic stellate cells. Aging (Albany NY) 13:957-972 (2020). PubMed: 33290258
  • Goto H  et al. Phospho-Specific Antibody Probes of Intermediate Filament Proteins. Methods Enzymol 568:85-111 (2016). PubMed: 26795468
  • Haolong C  et al. Enterovirus 71 VP1 activates calmodulin-dependent protein kinase II and results in the rearrangement of vimentin in human astrocyte cells. PLoS One 8:e73900 (2013). WB, ICC/IF ; Human . PubMed: 24073199
  • Mähönen AJ  et al. Culture medium induced vimentin reorganization associates with enhanced baculovirus-mediated gene delivery. J Biotechnol 145:111-9 (2010). PubMed: 19903502

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