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Anti-Vimentin 抗体 [RV203] (ab8979)

Reviews (3) Submit a question References (30)
Immunohistochemistry (Frozen sections) - Anti-Vimentin antibody [RV203] (ab8979)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [RV203] (ab8979)
  • Immunohistochemistry (Frozen sections) - Anti-Vimentin antibody [RV203] (ab8979)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [RV203] (ab8979)
  • Flow Cytometry - Anti-Vimentin antibody [RV203] (ab8979)

Key features and details

  • Mouse monoclonal [RV203] to Vimentin
  • Suitable for: IHC-P, Flow Cyt, IHC-Fr
  • Reacts with: Human
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-Vimentin antibody [RV203]
    Vimentin 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [RV203] to Vimentin

  • 由来種

    Mouse

  • 特異性

    This antibody reacts exclusively with vimentin which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma.

  • アプリケーション

    適用あり: IHC-P, Flow Cyt, IHC-Frmore details

  • 種交差性

    交差種: Human
    非交差種: Mouse

  • 免疫原

    Recombinant full length protein corresponding to Bovine Vimentin. Cytoskeletal vimentin extract of calf lens.
    Database link: P08670

  • ポジティブ･コントロール

    • Mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma

  • 特記事項

    Vimentin (57 kDa) is the intermediate filament protein (IFP) of mesenchymal cells. This IFP however often deviates from the tissue-specific and developmentally regulated pattern of expression. Besides its typical expression in most cultured cells, vimentin is also expressed together with several other IFPs during early stages of development. As differentiation proceeds, vimentin is exchanged for the tissue-specific intermediate filament type. Also in cancers, vimentin is often expressed in addition to the tissue-specific IFP.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab8979の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
Use a concentration of 5 µg/ml.
Flow Cyt
1/100 - 1/200.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr
Use at an assay dependent concentration.

Recommended range is 1/100 - 1/200 for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent.
特記事項
IHC-P
Use a concentration of 5 µg/ml.
Flow Cyt
1/100 - 1/200.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr
Use at an assay dependent concentration.

Recommended range is 1/100 - 1/200 for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent.

ターゲット情報

  • 機能

    Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
    Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.

  • 組織特異性

    Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.

  • 関連疾患

    Cataract 30

  • 配列類似性

    Belongs to the intermediate filament family.

  • ドメイン

    The central alpha-helical coiled-coil rod region mediates elementary homodimerization.
    The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.

  • 翻訳後修飾

    Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
    O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
    S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.

  • 細胞内局在

    Cytoplasm.
  • Information by UniProt

  • 参照データベース

    • 製品の状態

      Vimentin is found in connective tissue and in the cytoskeleton.

    • 別名

      • CTRCT30 antibody
      • Epididymis luminal protein 113 antibody
      • FLJ36605 antibody
      • HEL113 antibody
      • VIM antibody
      • VIME_HUMAN antibody
      • Vimentin antibody
      see all

    画像

    • Immunohistochemistry (Frozen sections) - Anti-Vimentin antibody [RV203] (ab8979)
      Immunohistochemistry (Frozen sections) - Anti-Vimentin antibody [RV203] (ab8979)

      Immunohistochemistry with ab8979 on a frozen section of human kidney showing positive staining in blood vessels, glomeruli and connective tissue cells and no reactivity in epithelial cells.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [RV203] (ab8979)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [RV203] (ab8979)
      IHC image of ab8979 staining in Breast Cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab8979, µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
    • Immunohistochemistry (Frozen sections) - Anti-Vimentin antibody [RV203] (ab8979)
      Immunohistochemistry (Frozen sections) - Anti-Vimentin antibody [RV203] (ab8979)
      Immunohistochemistry on frozen section of human colon showing positive staining in connective tissue cells and no reactivity in epithelial cells
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [RV203] (ab8979)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [RV203] (ab8979)
      Immunohistochemistry on paraffin section of human colon
    • Flow Cytometry - Anti-Vimentin antibody [RV203] (ab8979)
      Flow Cytometry - Anti-Vimentin antibody [RV203] (ab8979)
      Overlay histogram showing HeLa cells stained with ab8979 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8979, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Triton used under the same conditions.

    参考文献 (30)

    ab8979 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab8979 は 30 報の論文で使用されています。

    • Lu K  et al. HMGB2 upregulation promotes the progression of hepatocellular carcinoma cells through the activation of ZEB1/vimentin axis. J Gastrointest Oncol 14:2178-2191 (2023). PubMed: 37969822
    • Cao C  et al. Propofol prevents the aggressive progression of oral squamous cell carcinoma via regulating circ_0005623/miR-195-5p/HOXB7 axis. Biotechnol Appl Biochem 69:1015-1028 (2022). PubMed: 33894003
    • Sun B  et al. miR-2392 functions as tumour suppressor and inhibits malignant progression of hepatocellular carcinoma via directly targeting JAG2. Liver Int 42:1658-1673 (2022). PubMed: 35485355
    • Lv H  et al. hsa_circ_0000518 Facilitates Non-Small-Cell Lung Cancer Progression via Moderating miR-330-3p and Positively Regulating SLC1A5. J Immunol Res 2022:4996980 (2022). PubMed: 35874898
    • Bond A  et al. Development and Preliminary Testing of Porcine Blood-Derived Endothelial-like Cells for Vascular Tissue Engineering Applications: Protocol Optimisation and Seeding of Decellularised Human Saphenous Veins. Int J Mol Sci 23:N/A (2022). PubMed: 35743073
    View all Publications for this product

    レビューと Q&A

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    レビューを送る 質問を送る

    1-3 of 3 Abreviews or Q&A

    Immunocytochemistry abreview for Anti-Vimentin antibody [RV203]

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    African green monkey Cultured Cells (Vero cells)
    Permeabilization
    Yes - Saponin 0.5%
    Specification
    Vero cells
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Mar 07 2022

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Vimentin antibody [RV203]

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (A549)
    Permeabilization
    Yes - Saponin 0.5%
    Specification
    A549
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 May 14 2021

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Vimentin antibody [RV203]

     Good
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Reacts exclusively with vimentin)
    Specification
    Reacts exclusively with vimentin
    Fixative
    Methanol
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% BSA
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Jan 24 2007

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