Anti-Vimentin 抗体 [RV202] - Cytoskeleton Marker

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (AB8978)

Immunohistochemistry analysis of formalin fixed, paraffin embedded section of human small intestine labeling Vimentin with ab8978; showing positive staining in connective tissue cells and no reactivity in epithelial cells.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (AB8978)

ab8978 staining Vimentin in wild-type HAP1 cells (top panel) and VIM knockout HAP1 cells (bottom panel). The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab8978 at 1μg/ml and ab202272 (Rabbit monoclonal [EP1332Y] to alpha Tubulin (Alexa Fluor® 594)) at 1/250 dilution overnight at +4°C, followed by a further incubation at room temperature for 1h with ab150117 (Goat secondary antibody to Mouse IgG (Alexa Fluor® 488)) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• ICC/IF

PubMed

Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (AB8978)

Silencing of Lamin A/C in unmethylated neuroblastoma cells induces changes in different cytoskeletal components

Immunofluorescence staining showing changes in SK-N-SH-lamin-A/C-shRNA compared with SK-N-SH-scramble-shRNA in (A) β-actin filaments, (B) F-actin filaments. (C) Vimentin filaments, and (D) α-tubulin. Lamin A/C is shown in green; β-actin, F-actin, vimentin, and α-tubulin are shown in red.DNA is stained in blue (DAPI). Scale bar, 10μM.

Vimentin is detected using ab8978 at 1/100 dilution. Neuroblastoma cells were fixed with 4% paraformaldehyde and permeabilized using 0.5% Triton X-100.

(From Figure 5C of Rauschert et al)

Rauschert, I. et al PLoS One. 2017 Apr 19;12(4):e0175953. doi: 10.1371/journal.pone.0175953. eCollection 2017 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (AB8978)

Immunohistochemistry analysis of formalin fixed, paraffin embedded section of human spleen labeling Vimentin with ab8978, showing positive staining in connective tissue cells and lymphoid cells.

• IHC-Fr

AbReview14833****

Immunohistochemistry (Frozen sections) - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (AB8978)

ab8978 staining Vimentin in human lung tissue section by Immunohistochemistry (Frozen sections). Tissue samples were fixed with formaldehyde and blocking with 5% commercially available blocking agent was performed at 37⁰C for 15 minutes. The sample was incubated with primary antibody (1/250) at 37⁰C for 1 hour. A HRP-conjugated Goat polyclonal to mouse IgG was used as secondary antibody at 1/1000 dilution.

This image is courtesy of an anonymous abreview.

• ICC/IF

AbReview15980****

Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (AB8978)

ab8978 staining vimentin in human pancreatic adenocarcinoma cells by immunocytochemistry/ immunofluorescence. Cells were PFA fixed and permeabilized in 0.2% Triton X prior to blocking in 3% BSA for 30 minutes at 24°C. The primary antibody was diluted 1/200 and incubated with the sample for 16 hours at 21°C. Alexa fluor® 488 mouse polyclonal to mouse Ig, diluted 1/300 was used as the secondary antibody.

This image is courtesy of an Abreview submitted by Jennifer Dembinski

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (AB8978)

Paraffin-embedded human colon tissue stained for Vimentin using ab8978 at 1/100 dilution in immunohistochemical analysis.

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (AB8978)

Overlay histogram showing HeLa cells stained with ab8978 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8978, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This anti-Vimentin antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Triton used under the same conditions.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (AB8978)

Immunohistochemistry analysis of formalin fixed, paraffin embedded section of human placenta labeling Vimentin with ab8978, showing positive staining in connective tissue cells and no reactivity in epithelial cells.

• IHC-P

AbReview52045****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (AB8978)

ab8978 staining Vimentin in human fetal kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with CAS-Block for 1 hour at 25°C; antigen retrieval was by heat mediation using OmniPrep (pH 9). Samples were incubated with primary antibody (1/500) for 1 hour at 25°C. An Alexa Fluor® 555-conjugated Donkey polyclonal (1/200) was used as the secondary antibody.

This image is courtesy of an anonymous abreview.

• ICC/IF

Collaborator

Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (AB8978)

IHC-FoFr image of vimentin staining on rat injured cortical sections using ab8978 (1 : 500). The brain was perfusion fixed using 4% PFA and the sections were permeabilized using 0.1% TritonX in 0.1% PBS. The sections were then blocked using 10% donkey serum for 1 hour at 24°C. ab8978 was diluted 1 : 500 and incubated with sections for 24 hours using 4°C. The secondary antibody used was donkey polyclonal to rabbit IgG conjugated to Alexa Fluor 488.

This image is courtesy of Dr. Ruma Raha-Chowdhury, Cambridge University, United Kingdom.

• IHC-Fr

AbReview24195****

Immunohistochemistry (Frozen sections) - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (AB8978)

IHC-Fr image of Ed18 rat stained with ab8978. Fresh frozen sections were incubated in 10% normal donkey serum in 0.1% PBS- and 0.3% triton X100 for 1h to permeabilise the tissues and block non-specific protein-protein interactions. The sectons were then incubated with the ab8978 (1μg/ml) and ferroportin overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 568 (red) donkey anti-mouse at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM. Vimentin expressed in the gut muscles.

Image courtesy of an AbReview submitted by Dr Ruma Raha-Chowdhury

• IHC-Fr

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Immunohistochemistry (Frozen sections) - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (AB8978)

Immunohistochemistry analysis of frozen section of pig colon labeling Vimentin with ab8978, showing positive staining in connective tissue cells and no reactivity in epithelial cells. Nuclear staining with DAPI.

• IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (AB8978)

Immunofluorescence staining images of 9 day old zebrafish embryos. ab8978 reacts with in connective tissue cells and bloodvessels. Frozen sample treated with Acetone : Methanol 1 : 1, antibody diluted 1/100 and incubated for 45 minutes at room temperature.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (AB8978)

Immunohistochemistry analysis of frozen section of zebrafish embryo labeling Vimentin with ab8978.

• IHC-P

AbReview52345****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (AB8978)

ab8978 staining Vimentin in dog soft tissue sarcoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 15% serum for 1 hour at 20°C; antigen retrieval was by heat mediation in a Tris/EDTA pH9 buffer. Samples were incubated with primary antibody (1/100 in TBS) for 18 hours at 20°C. A Alexa Fluor® 647-conjugated Goat anti-mouse IgG polyclonal (1/400) was used as the secondary antibody.

This image is courtesy of an anonymous abreview.

• IHC-P

AbReview63656****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (AB8978)

Immunohistochemistry analysis (Formalin/PFA-fixed paraffin-embedded sections) of 10% normal buffered formalin-fixed pig lung tissue. Stained with ab8978 at 1/300 dilution. Secondary antibody used was CY3 conjugated donkey anti-mouse secondary antibody at 1/200 dilution. Blocking was done with 5% serum for 1 hour at 22°C. The sample was incubated with the primary antibody for 16 hours at 4°C. Antigen retrieval method was heat mediated, TE pH9.

This image is courtesy of an anonymous Abreview

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (AB8978)

Immunohistochemistry analysis of frozen section of pig colon labeling Vimentin with ab8978, showing positive staining in connective tissue cells and no reactivity in epithelial cells.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (AB8978)

Immunohistochemistry analysis of frozen section of zebrafish embryo labeling Vimentin with ab8978.

• WB

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Western blot - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (AB8978)

All lanes:

Western blot - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978)

Lane 1:

NIH3T3 cell lysate

Lane 2:

Normal human dermal fibroblasts cell extracts.

Predicted band size: 53 kDa

Observed band size: 57 kDa

false