AB188499

Anti-Vimentin 抗体

Anti-Vimentin antibody

Lab Essentials
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(5 Publications)

Rabbit Polyclonal Vimentin antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 5 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human VIM.

別名を表示する

Vimentin, VIM

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody (AB188499)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody (AB188499)

Immunofluorescent analysis of HeLa cells labeling Vimentin with ab188499 at 1/5000 dilution (red). Counter stained with a monoclonal antibody to Lamin A + C (green). DNA is stained blue.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody (AB188499)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Vimentin (green) using ab188499 at 1/5000 dilution in ICC/IF. Counterstained with mouse mAb to Actin at 1/500 dilution (red). Blue is DAPI staining of nuclear DNA.

Supplier Data

Western blot - Anti-Vimentin antibody (AB188499)

All lanes:

Western blot - Anti-Vimentin antibody (ab188499) at 1/5000 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lane 2:

SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate

Lane 3:

HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate

Lane 4:

NIH/3T3 (mouse embyro fibroblast cell line) whole cell lysate

Predicted band size: 53 kDa

false

Supplier Data

Western blot - Anti-Vimentin antibody (AB188499)

All lanes:

Western blot - Anti-Vimentin antibody (ab188499) at 1/10000 dilution

All lanes:

HeLa cell extract

Predicted band size: 53 kDa

true

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Human

アプリケーション

ICC/IF, WB

applications

免疫原

Recombinant Full Length Protein corresponding to Human VIM.

P08670

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/5000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mammals": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製度

Whole antiserum

バッファー組成

Preservative: 0.07% Sodium azide

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Plays a role in cell directional movement, orientation, cell sheet organization and Golgi complex polarization at the cell migration front (By similarity). Protects SCRIB from proteasomal degradation and facilitates its localization to intermediate filaments in a cell contact-mediated manner (By similarity). May promote axon outgrowth and motor fiber repair via DSP-mediated recruitment to outgrowth tips (By similarity).. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.

See full target information VIM

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文献 (5)

Recent publications for all applications. Explore the full list and refine your search

Journal of virology 97:e0087823 PubMed37905840

2023

Identification of host dependency factors involved in SARS-CoV-2 replication organelle formation through proteomics and ultrastructural analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Felix Pahmeier,Teresa-Maria Lavacca,Sarah Goellner,Christopher J Neufeldt,Vibhu Prasad,Berati Cerikan,Sreejith Rajasekharan,Giulia Mizzon,Uta Haselmann,Charlotta Funaya,Pietro Scaturro,Mirko Cortese,Ralf Bartenschlager

Cell host & microbe 28:853-866.e5 PubMed33245857

2020

Integrative Imaging Reveals SARS-CoV-2-Induced Reshaping of Subcellular Morphologies.

Applications

Unspecified application

Species

Unspecified reactive species

Mirko Cortese,Ji-Young Lee,Berati Cerikan,Christopher J Neufeldt,Viola M J Oorschot,Sebastian Köhrer,Julian Hennies,Nicole L Schieber,Paolo Ronchi,Giulia Mizzon,Inés Romero-Brey,Rachel Santarella-Mellwig,Martin Schorb,Mandy Boermel,Karel Mocaer,Marianne S Beckwith,Rachel M Templin,Viktoriia Gross,Constantin Pape,Christian Tischer,Jamie Frankish,Natalie K Horvat,Vibor Laketa,Megan Stanifer,Steeve Boulant,Alessia Ruggieri,Laurent Chatel-Chaix,Yannick Schwab,Ralf Bartenschlager

The Journal of pharmacology and experimental thera 370:416-426 PubMed31248980

2019

Platelet-Specific Deletion of Cyclooxygenase-1 Ameliorates Dextran Sulfate Sodium-Induced Colitis in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Angela Sacco,Annalisa Bruno,Annalisa Contursi,Melania Dovizio,Stefania Tacconelli,Emanuela Ricciotti,Paloma Guillem-Llobat,Tania Salvatore,Luigia Di Francesco,Rosa Fullone,Patrizia Ballerini,Vincenzo Arena,Sara Alberti,Guizhu Liu,Yanjun Gong,Alessandro Sgambato,Carlo Patrono,Garret A FitzGerald,Ying Yu,Paola Patrignani

Molecular medicine reports 18:2753-2761 PubMed30015869

2018

miR-577 suppresses cell proliferation and epithelial-mesenchymal transition by regulating the WNT2B mediated Wnt/β-catenin pathway in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Wang,Liwei Sun,Jinduo Li,Rong Jiang

Oncology research 26:529-536 PubMed28653599

2017

[ARTICLE WITHDRAWN] MicroRNA-539 Inhibits the Epithelial-Mesenchymal Transition of Esophageal Cancer Cells by Twist-Related Protein 1-Mediated Modulation of Melanoma-Associated Antigen A4.

Applications

Unspecified application

Species

Unspecified reactive species

Zhili Cao,Xiang Zheng,Lei Cao,Naixin Liang

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

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For licensing inquiries, please contact partnerships@abcam.com

Anti-Vimentin 抗体

Anti-Vimentin antibody

Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody (AB188499)

Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody (AB188499)

Western blot - Anti-Vimentin antibody (AB188499)

Western blot - Anti-Vimentin antibody (AB188499)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製度

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (5)

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Abcam product promise