Anti-Vimentin 抗体 (ab11256)
Key features and details
- Goat polyclonal to Vimentin
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Vimentin antibody
Vimentin 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to Vimentin -
由来種
Goat -
特異性
No reactivity was observed with the other families of intermediate filaments, including, desmin, keratin, neurofilaments and glial filaments.The antibody localizes vimentin in normal and pathological tissue of mesenchymal derivation. The antibody shows wide cross-reactivity among mammalian species. -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: a wide range of other species
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免疫原
Tissue, cells or virus corresponding to Human Vimentin. Vimentin from cultured human foreskin fibroblasts.
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特記事項
If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use. This goat antiserum was maintained at pH 5.0 for 40 minutes to meet U.S.D.A. requirements.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.097% Sodium azide
Constituent: Whole serum -
Concentration information loading... -
精製度
Whole antiserum -
特記事項（精製）
The antiserum has been treated to remove lipoproteins. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab11256の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | (1) |
1/20.
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| 特記事項 |
|---|
|
IHC-P
1/20. |
ターゲット情報
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機能
Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. -
組織特異性
Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines. -
関連疾患
Cataract 30 -
配列類似性
Belongs to the intermediate filament family. -
ドメイン
The central alpha-helical coiled-coil rod region mediates elementary homodimerization.
The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex. -
翻訳後修飾
Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 7431 Human
- Omim: 193060 Human
- SwissProt: P08670 Human
- Unigene: 455493 Human
- Unigene: 691131 Human
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製品の状態
Vimentin is found in connective tissue and in the cytoskeleton. -
別名
- CTRCT30 antibody
- Epididymis luminal protein 113 antibody
- FLJ36605 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody (ab11256)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of 0.1% trypsin treated human skin tissue labelling Vimentin with ab11256 at 1/20 dilution. FITC-conjugated rabbit anti-goat IgG (whole molecule) was used as the secondary antibody (1/20 dilution).
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (32)
ab11256 は 32 報の論文で使用されています。
- Citarella A et al. Hedgehog-GLI and Notch Pathways Sustain Chemoresistance and Invasiveness in Colorectal Cancer and Their Inhibition Restores Chemotherapy Efficacy. Cancers (Basel) 15:N/A (2023). PubMed: 36900263
- Zhang F et al. Identification of a Feed-Forward Loop Between 15(S)-HETE and PGE2 in Human Amnion at Parturition. J Lipid Res 63:100294 (2022). PubMed: 36206855
- Tian Y et al. Immunosuppressants Tacrolimus and Sirolimus revert the cardiac antifibrotic properties of p38-MAPK inhibition in 3D-multicellular human iPSC-heart organoids. Front Cell Dev Biol 10:1001453 (2022). PubMed: 36438566
- Lewis-Israeli YR et al. Generating Self-Assembling Human Heart Organoids Derived from Pluripotent Stem Cells. J Vis Exp N/A:N/A (2021). PubMed: 34605811
- Fan X et al. Hsa_circRNA_0045861 promotes renal injury in ureteropelvic junction obstruction via the microRNA-181d-5p/sirtuin 1 signaling axis. Ann Transl Med 9:1571 (2021). PubMed: 34790777