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AB109516

Anti-Villin 抗体 [EPR3490]

Anti-Villin antibody [EPR3490]

5

(1 Review)

|

(5 Publications)

Rabbit Recombinant Monoclonal Villin antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 5 publications.

別名を表示する

VIL, VIL1, Villin-1

2 Images
Western blot - Anti-Villin antibody [EPR3490] (AB109516)
  • WB

Unknown

Western blot - Anti-Villin antibody [EPR3490] (AB109516)

All lanes:

Western blot - Anti-Villin antibody [EPR3490] (ab109516) at 1/10000 dilution

Lane 1:

Human small intestine lysate at 10 µg

Lane 2:

HT-29 lysate at 10 µg

Lane 3:

Caco-2 lysate at 10 µg

Predicted band size: 93 kDa

Observed band size: 95 kDa

false

Flow Cytometry (Intracellular) - Anti-Villin antibody [EPR3490] (AB109516)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Villin antibody [EPR3490] (AB109516)

Intracellular flow cytometric analysis of Villin in permeabilized HT-29 cells, using ab109515 at a dilution of 1/10 (red) or a rabbit IgG (negative) (green).

関連する標識済み抗体及び組成の異なる製品 (1)

  • Carrier free

    Anti-Villin antibody [EPR3490] - BSA and Azide free

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR3490

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

Flow Cyt (Intra), WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<p><a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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製品の詳細

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
保管に関する情報
Stable for 12 months at -20°C
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Epithelial cell-specific Ca(2+)-regulated actin-modifying protein that modulates the reorganization of microvillar actin filaments. Plays a role in the actin nucleation, actin filament bundle assembly, actin filament capping and severing. Binds phosphatidylinositol 4,5-bisphosphate (PIP2) and lysophosphatidic acid (LPA); binds LPA with higher affinity than PIP2. Binding to LPA increases its phosphorylation by SRC and inhibits all actin-modifying activities. Binding to PIP2 inhibits actin-capping and -severing activities but enhances actin-bundling activity. Regulates the intestinal epithelial cell morphology, cell invasion, cell migration and apoptosis. Protects against apoptosis induced by dextran sodium sulfate (DSS) in the gastrointestinal epithelium. Appears to regulate cell death by maintaining mitochondrial integrity. Enhances hepatocyte growth factor (HGF)-induced epithelial cell motility, chemotaxis and wound repair. Upon S.flexneri cell infection, its actin-severing activity enhances actin-based motility of the bacteria and plays a role during the dissemination.
See full target information VIL1
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文献 (5)

Recent publications for all applications. Explore the full list and refine your search

Tissue engineering and regenerative medicine 19:403-415 PubMed35122584

2022

Application of Bone Marrow Mesenchymal Stem Cells Effectively Eliminates Endotoxemia to Protect Rat from Acute Liver Failure Induced by Thioacetamide.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Jiang,Geng Xia,Bo Yang,Hong-Wei Zhang,Yue-Shan Yin,Cheng-Wei Tang,Jin-Hui Yang

Molecular therapy. Methods & clinical development 17:49-57 PubMed31890740

2020

Establishment of SLC15A1/PEPT1-Knockout Human-Induced Pluripotent Stem Cell Line for Intestinal Drug Absorption Studies.

Applications

Unspecified application

Species

Unspecified reactive species

Kanae Kawai,Ryosuke Negoro,Moe Ichikawa,Tomoki Yamashita,Sayaka Deguchi,Kazuo Harada,Kazumasa Hirata,Kazuo Takayama,Hiroyuki Mizuguchi

Scientific reports 8:16393 PubMed30401824

2018

Aggregated SOD1 causes selective death of cultured human motor neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Benkler,Alison L O'Neil,Susannah Slepian,Fang Qian,Paul H Weinreb,Lee L Rubin

Biochemical and biophysical research communications 472:631-6 PubMed26966071

2016

Modeling of drug-mediated CYP3A4 induction by using human iPS cell-derived enterocyte-like cells.

Applications

Flow Cyt

Species

Human

Ryosuke Negoro,Kazuo Takayama,Yasuhito Nagamoto,Fuminori Sakurai,Masashi Tachibana,Hiroyuki Mizuguchi

Scientific reports 5:16479 PubMed26559489

2015

Generation of enterocyte-like cells from human induced pluripotent stem cells for drug absorption and metabolism studies in human small intestine.

Applications

Flow Cyt

Species

Human

Tatsuya Ozawa,Kazuo Takayama,Ryota Okamoto,Ryosuke Negoro,Fuminori Sakurai,Masashi Tachibana,Kenji Kawabata,Hiroyuki Mizuguchi
View all publications
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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。
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