AB180188

Anti-VGluT1 抗体 [EPR10953] - C-terminal

Name: Anti-VGluT1 抗体 [EPR10953] - C-terminal (ab180188) | Abcam
Brand: Abcam Limited
SKU: AB180188
Price: 40000 JPY
Availability: InStock
Rating: 5 (7 reviews)

Anti-VGluT1 antibody [EPR10953] - C-terminal

RabMAb
Recombinant
詳細を見る

(7 Reviews)

(13 Publications)

Rabbit Recombinant Monoclonal VGluT1 antibody. C-terminal. Suitable for ICC/IF, WB and reacts with Mouse, Human, Rat samples. Cited in 13 publications.

別名を表示する

BNPI, VGLUT1, SLC17A7, Vesicular glutamate transporter 1, VGluT1, Brain-specific Na(+)-dependent inorganic phosphate cotransporter, Solute carrier family 17 member 7

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-VGluT1 antibody [EPR10953] - C-terminal (AB180188)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-VGluT1 antibody [EPR10953] - C-terminal (AB180188)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling VGluT1 with ab180188 at 1/200 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2 μg/mL) (Green). Confocal image showing positive staining in mouse primary neuron cell. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 dilution (4 μg/mL) followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 dilution (2 μg/mL) (Red). The nuclear counterstain was DAPI (Blue).

Supplier Data

Western blot - Anti-VGluT1 antibody [EPR10953] - C-terminal (AB180188)

All lanes:

Western blot - Anti-VGluT1 antibody [EPR10953] - C-terminal (ab180188) at 1/1000 dilution

Lane 1:

Fetal brain lysate at 10 µg

Lane 2:

Neuro-2a (Mouse neuroblastoma cell line) lysate at 10 µg

Lane 3:

C6 (Rat glial tumor cell line) lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 61 kDa

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Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR10953

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Rat, Human

アプリケーション

ICC/IF, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.35% Sodium citrate, 0.17% Sodium chloride, 0.05% BSA, 0.03% EDTA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

VGluT1 or Vesicular Glutamate Transporter 1 is an important protein for transporting glutamate in the brain. It plays an important role in packaging glutamate into synaptic vesicles. This protein has an approximate mass of 61 kDa. Scientists find VGluT1 expression mainly in the central nervous system especially in areas involved in synaptic transmission and neural communication. VGluT1 localizes at the presynaptic terminals and enables glutamate to be stored in vesicles before release into the synaptic cleft.

Biological function summary

The function of VGluT1 centers on neurotransmitter regulation maintaining adequate synaptic glutamate levels. It does not work alone but works as part of a complex within the presynaptic vesicular organelles. This transport system enables efficient glutamate signaling essential for learning and memory. Through its action it supports important processes in brain development and synaptic plasticity indicating its role in cognitive functions.

Pathways

VGluT1 is essential in the glutamatergic signaling pathways which influence numerous neurotransmission activities. Its activity interlinks with proteins like synaptophysin and synapsins that help dock and release synaptic vesicles. Another associated pathway involves glutamate synthesis and recycling helping maintain excitatory neurotransmission balance. These pathways implicate VGluT1 in maintaining proper neural network connectivity and excitatory-inhibitory balance.

Altered VGluT1 function impacts conditions such as schizophrenia and amyotrophic lateral sclerosis (ALS). Researchers associate its dysregulation with impaired glutamate homeostasis leading to synaptic dysfunction. VGluT1 interacts with neuronal proteins such as EAAT2 involved in ALS where its malfunction results in motor neuron degeneration. In the context of schizophrenia altered VGluT1 expression disturbs glutamatergic neurotransmission contributing to symptoms due to synaptic imbalance.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Multifunctional transporter that transports L-glutamate as well as multiple ions such as chloride, proton, potassium, sodium and phosphate (PubMed : 10820226). At the synaptic vesicle membrane, mainly functions as an uniporter which transports preferentially L-glutamate but also phosphate from the cytoplasm into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells (By similarity). The L-glutamate or phosphate uniporter activity is electrogenic and is driven by the proton electrochemical gradient, mainly by the electrical gradient established by the vacuolar H(+)-ATPase across the synaptic vesicle membrane (By similarity). In addition, functions as a chloride channel that allows a chloride permeation through the synaptic vesicle membrane that affects the proton electrochemical gradient and promotes synaptic vesicles acidification (By similarity). Moreover, may function as a K(+)/H(+) antiport allowing to maintain the electrical gradient and to decrease chemical gradient and therefore sustain vesicular glutamate uptake (By similarity). The vesicular K(+)/H(+) antiport activity is electroneutral (By similarity). At the plasma membrane, following exocytosis, functions as a symporter of Na(+) and phosphate from the extracellular space to the cytoplasm allowing synaptic phosphate homeostasis regulation (PubMed : 10820226). The symporter activity is driven by an inside negative membrane potential and is electrogenic (By similarity). Is necessary for synaptic signaling of visual-evoked responses from photoreceptors (By similarity).

See full target information SLC17A7

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文献 (13)

Recent publications for all applications. Explore the full list and refine your search

Molecular biology of the cell 36:br4 PubMed39745872

2025

Parkinson disease-associated toxic exposures selectively up-regulate vesicular glutamate transporter vGlut2 in a model of human cortical neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Karis A Clark,Andrew J White,Wojciech Paslawski,Kellianne D Alexander,Shaoning Peng,Tracy L Young-Pearse,Per Svenningsson,Dennis J Selkoe,Gary P H Ho

International journal of molecular sciences 25: PubMed39684592

2024

A Comprehensive Functional Investigation of the Human Translocator Protein 18 kDa (TSPO) in a Novel Human Neuronal Cell Knockout Model.

Applications

Unspecified application

Species

Unspecified reactive species

Stefanie Bader,Tatjana Jahner,Anett Dörfelt,Doris Melchner,Iseline Cardon,Heiko I Siegmund,Christoph Brochhausen,Rainer Rupprecht,Vladimir M Milenkovic,Christian H Wetzel

Micromachines 15: PubMed38930702

2024

The Profile of Network Spontaneous Activity and Functional Organization Interplay in Hierarchically Connected Modular Neural Networks In Vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Yana Pigareva,Arseniy Gladkov,Vladimir Kolpakov,Victor B Kazantsev,Irina Mukhina,Alexey Pimashkin

Neural regeneration research 19:2240-2248 PubMed38488558

2024

In situ direct reprogramming of astrocytes to neurons via polypyrimidine tract-binding protein 1 knockdown in a mouse model of ischemic stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Meng Yuan,Yao Tang,Tianwen Huang,Lining Ke,En Huang

International journal of molecular sciences 25: PubMed38256041

2024

Mitochondrial and Cellular Function in Fibroblasts, Induced Neurons, and Astrocytes Derived from Case Study Patients: Insights into Major Depression as a Mitochondria-Associated Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Iseline Cardon,Sonja Grobecker,Selin Kücükoktay,Stefanie Bader,Tatjana Jahner,Caroline Nothdurfter,Kevin Koschitzki,Mark Berneburg,Bernhard H F Weber,Heidi Stöhr,Marcus Höring,Gerhard Liebisch,Frank Braun,Tanja Rothammer-Hampl,Markus J Riemenschneider,Rainer Rupprecht,Vladimir M Milenkovic,Christian H Wetzel

Molecular psychiatry 29:1217-1227 PubMed35732695

2022

Induced neural progenitor cells and iPS-neurons from major depressive disorder patients show altered bioenergetics and electrophysiological properties.

Applications

Unspecified application

Species

Unspecified reactive species

Julian Triebelhorn,Iseline Cardon,Kerstin Kuffner,Stefanie Bader,Tatjana Jahner,Katrin Meindl,Tanja Rothhammer-Hampl,Markus J Riemenschneider,Konstantin Drexler,Mark Berneburg,Caroline Nothdurfter,André Manook,Christoph Brochhausen,Thomas C Baghai,Sven Hilbert,Rainer Rupprecht,Vladimir M Milenkovic,Christian H Wetzel

Cell proliferation 54:e13085 PubMed34232542

2021

Infusion of hESC derived Immunity-and-matrix regulatory cells improves cognitive ability in early-stage AD mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Liu,Zongren Hou,Jun Wu,Kailun Liu,Da Li,Tingting Gao,Wenjing Liu,Bin An,Yun Sun,Fan Mo,Liu Wang,Yukai Wang,Jie Hao,Baoyang Hu

GeroScience 44:1047-1069 PubMed33983623

2021

Maternal stress programs a demasculinization of glutamatergic transmission in stress-related brain regions of aged rats.

Applications

Unspecified application

Species

Unspecified reactive species

Remy Verhaeghe,Vance Gao,Sara Morley-Fletcher,Hammou Bouwalerh,Gilles Van Camp,Francesca Cisani,Ferdinando Nicoletti,Stefania Maccari

Frontiers in neuroscience 12:985 PubMed30670942

2019

Neurochemical Modifications in the Hippocampus, Cortex and Hypothalamus of Mice Exposed to Long-Term High-Fat Diet.

Applications

Unspecified application

Species

Unspecified reactive species

Blanca Lizarbe,Ana Francisca Soares,Sara Larsson,João M N Duarte

Neuroscience 376:24-39 PubMed29452226

2018

Quantitative Proteomic Analysis Reveals Synaptic Dysfunction in the Amygdala of Rats Susceptible to Chronic Mild Stress.

Applications

Species

Unspecified reactive species

Mi Zhou,Zhao Liu,Jia Yu,Shuiming Li,Min Tang,Li Zeng,Haiyang Wang,Hong Xie,Li Peng,Haojun Huang,Chanjuan Zhou,Peng Xie,Jian Zhou

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-VGluT1 抗体 [EPR10953] - C-terminal

Anti-VGluT1 antibody [EPR10953] - C-terminal

Immunocytochemistry/ Immunofluorescence - Anti-VGluT1 antibody [EPR10953] - C-terminal (AB180188)

Western blot - Anti-VGluT1 antibody [EPR10953] - C-terminal (AB180188)

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Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (13)

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Abcam product promise