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    products/primary-antibodies/vglut1-antibody-ab77822.pdf

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Neuroscience Cell Adhesion Proteins Membrane Proteins
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Anti-VGluT1 抗体 (ab77822)

  • Datasheet
  • SDS
Reviews (2)Q&A (2)References (23)

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Western blot - Anti-VGluT1 antibody (ab77822)

    Key features and details

    • Rabbit polyclonal to VGluT1
    • Suitable for: WB
    • Reacts with: Rat
    • Isotype: IgG

    リコンビナント抗体で、ロット間での高い再現性を実現

    Product image
    Anti-VGluT1 antibody [EPR22269] (ab227805)
    • 異なるロット間での安定した再現性
    • 容易なスケールアップ
    • 評価試験による特異性の確認済み
    • 倫理基準に準拠 - アニマル・フリーの生産

    製品の概要

    • 製品名

      Anti-VGluT1 antibody
      VGluT1 一次抗体 製品一覧
    • 製品の詳細

      Rabbit polyclonal to VGluT1
    • 由来種

      Rabbit
    • 特異性

      Due to immunogen homology, the antibody may detect VGluT2 (100%) and VGluT3(95%).
    • アプリケーション

      適用あり: WBmore details
    • 種交差性

      交差種: Rat
      交差が予測される動物種: Mouse, Cow, Pigeon, Human, Zebrafish
    • 免疫原

      Synthetic peptide corresponding to Rat VGluT1 aa 150-250 conjugated to keyhole limpet haemocyanin.
      (Peptide available as ab93078)

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid
    • 保存方法

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • バッファー

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituents: 98.98% PBS, 1% BSA

      Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
    • Concentration information loading...
    • 精製度

      Immunogen affinity purified
    • ポリ/モノ

      ポリクローナル
    • アイソタイプ

      IgG
    • 研究分野

      • Neuroscience
      • Cell Adhesion Proteins
      • Membrane Proteins
      • Signal Transduction
      • Cytoskeleton / ECM
      • Cell Adhesion
      • Gap Junctions
      • Neuroscience
      • Development

    関連製品

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human VGluT1 protein (ab153436)

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab77822の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB (1)
    Use a concentration of 1 µg/ml. Detects a band of approximately 67 kDa (predicted molecular weight: 62 kDa).
    特記事項
    WB
    Use a concentration of 1 µg/ml. Detects a band of approximately 67 kDa (predicted molecular weight: 62 kDa).

    ターゲット情報

    • 機能

      Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate.
    • 組織特異性

      Expressed in several regions of the brain including amygdala, cerebellum, cerebral cortex, hippocampus, frontal lobe, medulla, occipital lobe, putamen and temporal lobe.
    • 配列類似性

      Belongs to the major facilitator superfamily. Sodium/anion cotransporter family. VGLUT subfamily.
    • 細胞内局在

      Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Membrane. Cell junction, synapse, synaptosome.
    • Target information above from: UniProt accession Q9P2U7 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 参照データベース

      • Entrez Gene: 518849 Cow
      • Entrez Gene: 57030 Human
      • Entrez Gene: 72961 Mouse
      • Entrez Gene: 116638 Rat
      • Omim: 605208 Human
      • SwissProt: A4FV52 Cow
      • SwissProt: Q9P2U7 Human
      • SwissProt: Q3TXX4 Mouse
      • SwissProt: Q62634 Rat
      • Unigene: 375616 Human
      • Unigene: 255631 Mouse
      • Unigene: 10267 Rat
      see all
    • 別名

      • BNPI antibody
      • Brain specific Na (+) dependent inorganic phosphate cotransporter antibody
      • Brain specific Na dependent inorganic phosphate cotransporter antibody
      • Brain-specific Na(+)-dependent inorganic phosphate cotransporter antibody
      • Slc17a7 antibody
      • Solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7 antibody
      • Solute carrier family 17 member 7 antibody
      • Vesicular glutamate transporter 1 antibody
      • VGLU1_HUMAN antibody
      • VGLUT 1 antibody
      • VGluT1 antibody
      see all

    画像

    • Western blot - Anti-VGluT1 antibody (ab77822)
      Western blot - Anti-VGluT1 antibody (ab77822)
      All lanes : Anti-VGluT1 antibody (ab77822) at 1 µg/ml

      Lane 1 : Rat Brain Tissue Lysate
      Lane 2 : Rat Cerebellum Tissue Lysate
      Lane 3 : Rat Cortex Tissue Lysate
      Lane 4 : Rat Forebrain

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 62 kDa
      Observed band size: 67 kDa why is the actual band size different from the predicted?
      Additional bands at: 41 kDa, 50 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 3 minutes


      Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

    プロトコール

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    データシートおよび資料

    • SDS download

    • Datasheet download

      Download

    参考文献 (23)

    ab77822 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab77822 は 23 報の論文で使用されています。

    • Aloi MS  et al. Microglia specific deletion of miR-155 in Alzheimer's disease mouse models reduces amyloid-β pathology but causes hyperexcitability and seizures. J Neuroinflammation 20:60 (2023). PubMed: 36879321
    • Albrecht NE  et al. Rapid 3D-STORM imaging of diverse molecular targets in tissue. Cell Rep Methods 2:100253 (2022). PubMed: 35880013
    • Peters JJ  et al. Molecular Characterization of AMPA-Receptor-Containing Vesicles. Front Mol Neurosci 14:754631 (2021). PubMed: 34720876
    • Garita-Hernandez M  et al. Control of Microbial Opsin Expression in Stem Cell Derived Cones for Improved Outcomes in Cell Therapy. Front Cell Neurosci 15:648210 (2021). PubMed: 33815066
    • DeMarco E  et al. Pyramidal Neurons of the Zebrafish Tectum Receive Highly Convergent Input From Torus Longitudinalis. Front Neuroanat 15:636683 (2021). PubMed: 33613200
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-4 of 4 Abreviews or Q&A

    Western blot abreview for Anti-VGluT1 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Lampreys Tissue lysate - whole (Spinal Cord)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    Spinal Cord
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Mar 06 2024

    Immunohistochemistry (Frozen sections) abreview for Anti-VGluT1 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Sample
    Zebrafish Tissue sections (Brain, axons)
    Specification
    Brain, axons
    Permeabilization
    Yes - Triton X
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Aug 26 2014

    Question

    Searching for a vGLUT1 and dopamine receptor antibody to work in hamster tissue.

    Read More

    Abcam community

    Verified customer

    Asked on Mar 08 2012

    Answer

    DISCOUNT CODES: ab5981 - ******
    ab104898 - ***********

    Expiration date: 9th July 2012 (for both discount codes)

    I am very pleased to hear you would like to accept our offer and test ab5981 and ab104898in hamster tissue]. This code will give you 1 free PRIMARY ANTIBODY per discount codebefore the expiration date. To redeem this offer, please submit an Abreview for how these antibodies perform when used on hamster tissue and include the appropriatecode in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

    Abcam Scientific Support

    Answered on Mar 08 2012

    Question

    Hello,   I am searching an antibody which recognizes BOTH VGluT1 and VGluT2 (preferentially equally well) on WBs. Could you please tell me if one of the following AB meets this criteria:   Anti-VGluT1 antibody (ab79774) Anti-VGLUT2 antibody (ab101760) Anti-VGLUT2 antibody (ab72310) Anti-VGLUT2 antibody (ab101756) Anti-VGLUT2 antibody [8G9.2] (ab79157) Anti-VGluT1 antibody (ab77822) Anti-VGluT1 antibody (ab72311) Anti-VGluT1 antibody (ab104899) Anti-VGluT1 antibody (ab104898)   Thank you!     Benjamin Wilhelm     Benjamin G. Wilhelm European Neuroscience Institute STED Microscopy of Synaptic Function Grisebachstr. 5 37077 Goettingen Germany Phone 0049-551-3913901 Fax   0049-551-3910653    

    Read More

    Abcam community

    Verified customer

    Asked on Nov 11 2011

    Answer

    Thank you for your inquiry. The above mentioned antibodies are all tested to be specific for either VGlut1 or VGlut2. The sequence homology is only 73% between the two VGlut proteins and the antibodies are all tested to be specific for either VGlut1 or VGlut2. I suggest to purchase two antibodies raised in the same host, one against VGlut1 and one against VGlut2 and then maybe mix them to simultaneously probe for both proteins. But I have to admit that using them separately would be much cleaner and also better laboratory practice. I am sorry I could not provide a product that suits your purposes.

    Read More

    Abcam Scientific Support

    Answered on Nov 11 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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