Anti-VGF 抗体 (ab115609)
Key features and details
- Rabbit polyclonal to VGF
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-VGF antibody
VGF 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to VGF -
由来種
Rabbit -
特異性
The immunogen is after amino acid 577 of O15240, so the antibody should only cross-react with full-length VGF and none of the processed peptides. -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
17 amino acid peptide near the carboxy terminus of the Human VGF.
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ポジティブ･コントロール
- Human Brain Cortex tissue; Human Brain tissue lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.4
Preservative: 0.02% Sodium azide
Constituent: 0.99% PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab115609の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 67 kDa.
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| IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 67 kDa. |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
May be involved in the regulation of cell-cell interactions or in synatogenesis during the maturation of the nervous system.
NERP peptides are involved in the control of body fluid homeostasis by regulating vasopressin release. -
組織特異性
Central and peripheral nervous systems, synthesized exclusively in neuronal and neuroendocrine cells. -
翻訳後修飾
Multiple peptides are derived from VGF, with activities in synaptic plasticity, antidepression, penile erection, autonomic activation, and increases in energy expenditure. -
細胞内局在
Secreted. Cytoplasmic vesicle > secretory vesicle. Stored in secretory vesicles and then secreted, NERP peptides colocalize with vasopressin in the storage granules of hypothalamus. - Information by UniProt
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参照データベース
- Entrez Gene: 7425 Human
- Entrez Gene: 381677 Mouse
- Entrez Gene: 29461 Rat
- Omim: 602186 Human
- SwissProt: O15240 Human
- SwissProt: P20156 Rat
- Unigene: 587325 Human
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別名
- NERP-1 antibody
- NERP-2 antibody
- Nerve growth factor inducible antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VGF antibody (ab115609)ab115609, at 5µg/ml, staining VGF in Formalin-fixed, Paraffin-embedded Human Brain cortex tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
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Western blot - Anti-VGF antibody (ab115609)Lane 1 : Anti-VGF antibody (ab115609) at 0.5 µg/ml
Lane 2 : Anti-VGF antibody (ab115609) at 1 µg/ml
All lanes : Human Brain tissue lysate
Predicted band size: 67 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab115609 は 1 報の論文で使用されています。
- Marwitz S et al. Epigenetic modifications of the VGF gene in human non-small cell lung cancer tissues pave the way towards enhanced expression. Clin Epigenetics 9:123 (2017). PubMed: 29209432