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AB316816

Anti-VEGFD 抗体 [EPR24690-17] - BSA and Azide free (Detector)

Anti-VEGFD antibody [EPR24690-17] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal VEGFD antibody. Carrier free. Suitable for sELISA and reacts with Rat samples.

別名を表示する

Figf, Vascular endothelial growth factor D, VEGF-D, c-Fos-induced growth factor, FIGF

1 Images
Sandwich ELISA - Anti-VEGFD antibody [EPR24690-17] - BSA and Azide free (Detector) (AB316816)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-VEGFD antibody [EPR24690-17] - BSA and Azide free (Detector) (AB316816)

Sandwich ELISA of ab316815 with the capture antibody ab244740 dilution at 2 ug/mL and detector antibody ab316816 dilution at 0.5 ug/mL.

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR24690-17

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Rat

アプリケーション

sELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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製品の詳細

ab316816 is a BSA and Azide free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Rat VEGFD. The recommended pair for sandwich ELISA is:

Capture: ab244740, Rat VEGFD Capture Antibody (unconjugated)

Detector: ab316816, Rat VEGFD Detector Antibody (unconjugated)

The reference range value is 3.91-1000 pg/mL.

The recommended antibody orientation is based on internal optimization for ELISAbased assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Constituents: PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

VEGFD also known as vascular endothelial growth factor D plays a role in angiogenesis and lymphangiogenesis by interacting with VEGF receptors. This protein has a molecular mass of approximately 32 kDa. It is typically expressed in the lungs heart skeletal muscle and small intestine. VEGFD undergoes several proteolytic processes to gain full activity highlighting its functional complexity in the body.
Biological function summary

Vascular endothelial growth factor D promotes the proliferation and migration of endothelial cells facilitating vascular formation and remodeling. It forms part of the VEGF protein family which is known for its involvement in various vascular processes. The protein's action is largely mediated through its binding to VEGFR-2 and VEGFR-3. This binding activates downstream signaling pathways that contribute to the growth and maintenance of blood and lymphatic vessels.

Pathways

The functioning of VEGFD is important in the vascular endothelial growth factor signaling pathway and the PI3K-Akt signaling pathway. These pathways are essential for the regulation of cellular processes such as survival growth and motility of endothelial cells. VEGFD is closely related to other members of the VEGF family including VEGFA and VEGFC which similarly interact with VEGF receptors to regulate vascular development and function.

Abnormal levels or activity of VEGFD can lead to pathological conditions such as cancer and lymphedema. In several cancers VEGFD is implicated in tumor angiogenesis aiding the tumor in acquiring nutrients and oxygen. Furthermore in lymphedema issues related to lymphatic vessel development can involve VEGFD alongside VEGFC contributing to fluid retention and tissue swelling. These associations highlight the importance of understanding VEGFD's functions in various pathological contexts.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Growth factor active in angiogenesis, lymphangiogenesis and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in the formation of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates VEGFR-3 (Flt4) receptor (By similarity).
See full target information Vegfd
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組合せ製品

Assay Kits

AB233625

Human VEGF-D ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human VEGF-D ELISA Kit (AB233625)

  • 0
  • 0
Primary Antibodies

AB303710

Anti-VEGFC antibody [EPR22854-144] - BSA and Azide free (Capture)

RabMAb|Recombinant

Sandwich ELISA - Anti-VEGFC antibody [EPR22854-144] - BSA and Azide free (Capture) (AB303710)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB32152

Anti-VEGF Receptor 1 antibody [Y103]

RabMAb|Recombinant|20ul selling size

Immunoprecipitation - Anti-VEGF Receptor 1 antibody [Y103] (AB32152)

  • 5
  • 21
Primary Antibodies

AB27278

Anti-VEGF Receptor 3 antibody

Western blot - Anti-VEGF Receptor 3 antibody (AB27278)

  • 4
  • 8

Abcam product promise

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