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Anti-VEGFA 抗体 (ab39250)

Reviews (1)Q&A (2)References (26)
Immunocytochemistry/ Immunofluorescence - Anti-VEGFA antibody (ab39250)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGFA antibody (ab39250)

Key features and details

  • Rabbit polyclonal to VEGFA
  • Suitable for: IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-VEGFA antibody [EP1176Y] - C-terminal (ab52917)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-VEGFA antibody
    VEGFA 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to VEGFA

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IHC-P, ICC/IFmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide within Human VEGFA aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: P15692

  • ポジティブ･コントロール

    • Tumor cells in hemangiosarcomas IF/ICC: HepG2 cell line

  • 特記事項

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab39250の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF
Use a concentration of 1 µg/ml.
特記事項
IHC-P
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF
Use a concentration of 1 µg/ml.

ターゲット情報

  • 機能

    Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.

  • 組織特異性

    Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed.

  • 関連疾患

    Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1) [MIM:603933]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.

  • 配列類似性

    Belongs to the PDGF/VEGF growth factor family.

  • 細胞内局在

    Secreted. VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • Folliculostellate cell-derived growth factor antibody
    • Glioma-derived endothelial cell mitogen antibody
    • MGC70609 antibody
    • MVCD1 antibody
    • Vascular endothelial growth factor A antibody
    • vascular endothelial growth factor A121 antibody
    • vascular endothelial growth factor A165 antibody
    • vascular endothelial growth factor antibody
    • Vascular permeability factor antibody
    • VEGF A antibody
    • Vegf antibody
    • VEGF-A antibody
    • VEGF120 antibody
    • Vegfa antibody
    • VEGFA_HUMAN antibody
    • VPF antibody
    see all

画像

  • Immunocytochemistry/ Immunofluorescence - Anti-VEGFA antibody (ab39250)
    Immunocytochemistry/ Immunofluorescence - Anti-VEGFA antibody (ab39250)

    ICC/IF image of ab39250 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39250, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGFA antibody (ab39250)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGFA antibody (ab39250)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human angiosarcoma stained with ab39250 at 1/100.

参考文献 (26)

ab39250 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab39250 は 26 報の論文で使用されています。

  • Klinsang T  et al. In Vitro Wound Healing Potential of a Fibroin Film Incorporating a Cannabidiol/2-Hydroxypropyl-β-cyclodextrin Complex. Pharmaceutics 15:N/A (2023). PubMed: 38140022
  • Dai Q  et al. miR-29c-5p knockdown reduces inflammation and blood-brain barrier disruption by upregulating LRP6. Open Med (Wars) 17:353-364 (2022). PubMed: 35799601
  • Ke B  et al. VEGFA promotes the occurrence of PLA2R-associated idiopathic membranous nephropathy by angiogenesis via the PI3K/AKT signalling pathway. BMC Nephrol 23:313 (2022). PubMed: 36114523
  • Kumthekar P  et al. A multi-institutional phase II trial of bevacizumab for recurrent and refractory meningioma. Neurooncol Adv 4:vdac123 (2022). PubMed: 36225651
  • Ma C  et al. Curcumol repressed cell proliferation and angiogenesis via SP1/mir-125b-5p/VEGFA axis in non-small cell lung cancer. Front Pharmacol 13:1044115 (2022). PubMed: 36467048
View all Publications for this product

レビューと Q&A

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1-3 of 3 Abreviews or Q&A

Immunohistochemistry - Free Floating abreview for Anti-VEGF antibody

 Good
Abreviews
abreview image
Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (brain)
Specification
brain
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Ms. Christina Whiteus

Verified customer

投稿 Oct 15 2010

Question

Phone call requesting testing discount for the anti-VEGF antibody (ab39250).

Read More
Answer

Thank you for contacting us and your interst in our products.
As discussed over the phone, the Anti-VEGF antibody (ab39250) has not as yet been used in western blotting as you are hoping to do. This does not mean that it would not work, simply that we have not had any reports supporting it yet and would therefore not be able to guarentee it.
I'd be happy to offer you the testing discount if you wanted to try this antibody. This offer means that if you test the antibody in Western blotting and share the results with us through an Abreview (no matter whether positive or negative) you would be eligible for a free primary antibody of your choice from our catalogue (or the equivalent price off any product in our catalogue). More information on this offer can be found here:
www.abcam.com/collaborationdiscount
The only constraint to this offer is that the antibody to be tested must be purchased, tested, the Abreview submitted and the free primary antibody claimed within a 4 month period.
As discussed over the phone, here is the discount code:
xxxxxxx

Please quote this code in the "Additional comments" section when submitting your Abreview. Once the Abreview has been submitted the code will become activated and you can quote the code when placing the order for your free product. This code expires on the 26th of November 2012.
I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

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Question

Hi, I was wondering if you could confirm if Abcam antibody AB39250 (VEGF) is targeted against VEGF-A, VEGF-B, VEGF-C, VEGF-D etc or does it react to all these variants?

Read More
Answer

ab39250 is against VEGFA (VEGF). We do not have any experimental data regarding the cross-reactivity of E2614 with VEGFB, VEGFC, or VEGFD. The percentage homology between the immunogen and the other variants of VEGF are as follows: VEGFB (P49765): 33% VEGFC (P49767): 60% VEGFD (O43915): 50%

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For licensing inquiries, please contact partnerships@abcam.com